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CIAS detection of Fasciola hepatica/F. gigantica intermediate forms in bovines from Bangladesh

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Abstract

Fascioliasis is an important food-borne parasitic zoonosis caused by two trematode species, Fasciola hepatica and Fasciola gigantica. The characterisation and differentiation of Fasciola populations is crucial to control the disease, given the different transmission, epidemiology and pathology characteristics of the two species. Lineal biometric features of adult liver flukes infecting livestock have been studied to characterise and discriminate fasciolids from Bangladesh. An accurate analysis was conducted to phenotypically discriminate between fasciolids from naturally infected bovines (cattle, buffaloes) throughout the country. Morphometric analyses were made with a computer image analysis system (CIAS) applied on the basis of standardised measurements and the logistic model of the body growth and development of fasciolids in the different host groups. Since it is the first ever comprehensive study of this kind undertaken in Bangladesh, the results are compared to pure fasciolid populations of F. hepatica from the European Mediterranean area and F. gigantica from Burkina Faso, geographical areas where both species do not co-exist. Principal component analysis showed that the biometric characteristics of fasciolids from Bangladesh are situated between F. hepatica and F. gigantica standard populations, indicating the presence of phenotypes of intermediate forms in Bangladesh. These results are analysed by considering the present emergence of animal fascioliasis, the local lymnaeid fauna, the impact of climate change, and the risk of human infection in the country.

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Authors and Affiliations

  1. Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    Syed Ali Ahasan, Emdadul Haque Chowdhury & Mohammad Rafiqul Islam

  2. Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100, Burjassot, Valencia, Spain

    Syed Ali Ahasan, M. Adela Valero, Raquel V. Peixoto, Lavinia Berinde, Miroslava Panova & Santiago Mas-Coma

  3. Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    Mohammad Taohidul Islam

  4. Department of Parasitology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    Mohammad Motahar Hussain Mondal

  5. Department of Microbiology, University of Medicine and Pharmacy “Iuliu Hatieganu”, Louis Pasteur street No. 6, Cluj-Napoca, 400394, Romania

    Lavinia Berinde

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  1. Syed Ali Ahasan
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  2. M. Adela Valero
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  3. Emdadul Haque Chowdhury
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Correspondence to M. Adela Valero.

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Ahasan, S.A., Valero, M.A., Chowdhury, E.H. et al. CIAS detection of Fasciola hepatica/F. gigantica intermediate forms in bovines from Bangladesh. Acta Parasit. 61, 267–277 (2016). https://doi.org/10.1515/ap-2016-0037

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