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Morphological, molecular and developmental characterization of the thelastomatid nematode Thelastoma bulhoesi (de Magalhães, 1900) (Oxyuridomorpha: Thelastomatidae) parasite of Periplaneta americana (Linnaeus, 1758) (Blattodea: Blattidae) in Japan

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Abstract

The American cockroach Periplaneta americana (Linnaeus, 1758) (Blattodea: Blattidae) has been spreading worldwide by commerce and has successfully adjusted to living with humans. There are many reports of thelastomatid parasitic nematode isolated from P. americana in many countries including USA, Canada, India, Argentina, Bulgaria, but not in Japan. We have investigated the parasitic nematodes in P. americana lab strain and field-captured individuals in Japan and found that Thelastoma bulhoesi (de Magalhães, 1900) (Oxyuridomorpha: Thelastomatidae) parasitizes with high infection rates. We described morphological, molecular, and developmental characters of the parasitic nematode because such information was missing despite it has been discovered more than one hundred years ago. We described morphometrics with DIC microscopy and fine structure of male and female adult with SEM observation. We also reveal the embryonic and postembryonic development of this nematode. This is the first report of a thelastomatid nematode isolated from American cockroach in Japan, and the data showed here is also very useful and fundamental for further analysis of the cockroach and parasite relations.

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Authors and Affiliations

  1. Department of Environmental Biology, College of Bioscience & Biotechnology, 1200 Matsumoto, Kasugai, Aichi, 487-8501, Japan

    Sota Ozawa, Cláudia S. L. Vicente, Kenji Ikeda & Koichi Hasegawa

  2. Instituto de Ecología y Sistemática, Carretera Varona 11835 e/ Oriente y Lindero, La Habana 19, CP 11900, Calabazar, Boyeros, La Habana, Cuba

    Jans Morffe

  3. ICAAM—Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Departamento de Biologia, Universidade de Évora, Núcleo da Mitra, Ap. 94, 7002-554, Évora, Portugal

    Cláudia S. L. Vicente

  4. HHMI and Division of Biology and Biological Engineering, California Institute of Technology, 1200 East California Blvd, Pasadena, CA, 91125, USA

    Ryoji Shinya

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  1. Sota Ozawa
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  2. Jans Morffe
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  3. Cláudia S. L. Vicente
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Correspondence to Koichi Hasegawa.

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Sota Ozawa and Jans Morffe contributed equally to this work and should be consider as first co-authors Author’s

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Ozawa, S., Morffe, J., Vicente, C.S.L. et al. Morphological, molecular and developmental characterization of the thelastomatid nematode Thelastoma bulhoesi (de Magalhães, 1900) (Oxyuridomorpha: Thelastomatidae) parasite of Periplaneta americana (Linnaeus, 1758) (Blattodea: Blattidae) in Japan. Acta Parasit. 61, 241–254 (2016). https://doi.org/10.1515/ap-2016-0034

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