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Molecular studies on larvae of Pseudoterranova parasite of Trichiurus lepturus Linnaeus, 1758 and Pomatomus saltatrix (Linnaeus, 1766) off Brazilian waters

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Abstract

Pseudoterranova larvae parasitizing cutlassfish Trichiurus lepturus and bluefish Pomatomus saltatrix from Southwest Atlantic coast of Brazil were studied in this work by morphological, ultrastructural and molecular approaches. The genetic analysis were performed for the ITS2 intergenic region specific for Pseudoterranova decipiens, the partial 28S (LSU) of ribosomal DNA and the mtDNA cox-1 region. We obtained results for the 28S region and mtDNA cox-1 that was amplified using the polymerase chain reaction and sequenced to evaluate the phylogenetic relationships between sequences of this study and sequences from the GenBank. The morphological profile indicated that all the nine specimens collected from both fish were L3 larvae of Pseudoterranova sp. The genetic profile confirmed the generic level but due to the absence of similar sequences for adult parasites on GenBank for the regions amplifyied, it was not possible to identify them to the species level. The sequences obtained presented 89% of similarity with Pseudoterranova decipiens (28S sequences) and Contracaecum osculatum B (mtDNA cox-1). The low similarity allied to the fact that the amplification with the specific primer for P. decipiens didn’t occur, lead us to conclude that our sequences don’t belong to P. decipiens complex.

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References

  • Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. 1990. Basic Local Alignment Search Tool. Journal of Molecular Biology, 215, 403–10. DOI: 10.1016/S0022-2836(05)80360-2

    Article  CAS  Google Scholar 

  • Barros G.C. Amato J.F.R. 1992. Larvas de Anisakídeos de Peixeespada, Trichiurus lepturus L., da costa do estado do Rio de Janeiro, Brasil. Revista Brasileira de Biologia, 53, 241–245

    Google Scholar 

  • Blouin M.S. 2002. Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer. International Journal for Parasitology, 32, 527–531

    Article  CAS  Google Scholar 

  • Borges J.N., Cunha L.F.G., Santos H.L.C., Monteiro-Neto C., Santos C.P. 2012. Morphological and molecular diagnosis of Anisakid Nematode larvae from Cutlassfish (Trichiurus lep-turus) off the coast of Rio de Janeiro, Brazil. PlosOne 7: e40447. DOI: 10.1371/journal.pone.0040447

    Google Scholar 

  • Bowles J., Blair D., McManus D.P. 1992. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology, 54, 165–173

    Article  CAS  Google Scholar 

  • Caldwell D.K., Caldwell M.C. 1989. Pygmy sperm whale Kogia breviceps (de Blainville, 1838); Dwarf sperm whale Kogia simus Owen, 1866. In: (Eds. S. H. Ridgway and S. R. Harrison) Handbook of Marine Mammals Vol. 4: River Dolphins and the Larger Toothed Whales. Academic Press, London, pp. 235–260

    Google Scholar 

  • Froese R., Pauly D. 2011. FishBase. World Wide Web electronic publication. http://www.fishbase.org - version (December 2011). Accessed 10 December 2011

    Google Scholar 

  • Felsenstein J. 1981. Evolutionary Trees from DNA Sequences: A Maximum Likelihood Approach. Journal of Molecular Biology Evolution, 17, 368–376

    Article  CAS  Google Scholar 

  • Haimovici M., Krug L.C. 1996. Fishery and biology of the enchova Pomatomus saltatrix in southern Brazil. Australian Journal of Marine & Freshwater Research, 47, 357–363. DOI:10. 1071/MF9960357

    Article  Google Scholar 

  • Kimura M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120. DOI: 10.1007/BF01731581

    Article  CAS  Google Scholar 

  • Luque J.L., Chaves N.D. 1999. Ecologia da comunidade de metazoários parasitos da anchova Pomatomus saltator (Linnaeus) (Osteichthyes, Pomatomidae) do litoral do estado do Rio de Janeiro, Brasil. Revista Brasileira de Zoologia, 16, 711–23. DOI: 10.1590/S0101-81751999000300010

    Article  Google Scholar 

  • Luque J.L., Aguiar J.C., Vieira F.M., Gibson D.I., Santos C.P. 2011. Checklist of Nematoda associated with fishes of Brazil. Zootaxa, 3082, 1–88

    Google Scholar 

  • Martins A.S., Haimovici M. 1997. Distribution, abundance and biological interactions of the cutlassfish Trichiurus lepturus in the southern Brasil subtropical convergence ecosystem. Fisheries Research, 30, 217–227. DOI: 10.1016/S0165-7836 (96)00566-8

    Article  Google Scholar 

  • Mattiucci S., Nascetti G. 2008. Advances and trends in molecular systematics of Anisakid Nematodes, with implications for their evolutionary, ecology and host-parasite co-evolutionary processes. In: (Eds. D. Rollinson and S. I. Hay) Advances in parasitology, vol. 66, Academic Press, London, 47–148

    Google Scholar 

  • McClelland G., Misra R.K., Martell D.J. 2000. Sapatial and temporal distributions of larval sealworm (Pseudoterranova decipiens, Nematoda: Anisakinae), in Hippoglossoides pla-tessoides (Pleuronectidae) in eastern Canada from 1980 to 1990. ICES Journal of Marine Science, 57, 69–88. DOI: 10.1006/jmsc.1999.0518

    Article  Google Scholar 

  • Muniz-Pereira L.C., Vieira F.M., Luque J. 2010. Checklist of helminth parasites of threatened vertebrate species from Brazil. Zootaxa, 2123, 1–45

    Article  Google Scholar 

  • Nadler S.A., D’Amelio S., Dailey M.D., Paggi L., Siu S., Sakanari J.A. 2005. Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern Pacific marine mammals. The Journal of Parasitology, 91, 1413–29. DOI: 10.1645/GE-522R.1

    Article  CAS  Google Scholar 

  • Paraguassú A.R., Luque J.L., Alves D.R. 2000. Aspectos quantitativos do parasitismo por larvas de anisakideos (Nematoda: Ascaridoidea: Anisakidae) no pargo, Pagrus pagrus (Linnaeus, 1758) (Osteichthyes: Sparidae) do litoral do estado do Rio de Janeiro, Brasil. Contribuições Avulsas sobre a Historia Natural do Brasil, 24, 1–8

    Google Scholar 

  • Pinto R.M., Muniz-Pereira L.C., Alves V.C., Siciliano S. 2004. First report of a helminth infection for Bryde’s whale, Balaenoptera edeni Anderson, 1878 (Cetacea, Balaenopteridae). Latin American Journal of Aquatic Mammals, 3, 167–170. DOI: 10.5597/lajam00062

    Article  Google Scholar 

  • Rêgo A.A., Vicente J.J., Santos C.P., Wekid, R.M. 1983. Parasitas de anchovas Pomatomus saltatrix (L.) do Rio de Janeiro. Ciência e Cultura, 35, 1329–1336

    Google Scholar 

  • Santos C.P., Lodi L. 1998. Occurrence of Anisakis physeteris Baylis, 1923 and Pseudoterranova sp. (Nematoda) in pygmy sperm whale Kogia breviceps (De Blainvillei, 1838) (Physeteridae) in northeastern coast of Brazil. Memórias do Instituto Oswaldo Cruz, 92, 187–188. DOI: 10.1590/S0074-0276199 8000200009

    Article  Google Scholar 

  • São Clemente S.C., Marques M.C., Serra-Freire N.M., Lucena F.P. 1995. Análise do parasitismo de peixe espada Trichiurus lepturus (L.) do litoral do Rio de Janeiro - Brasil. Parasitología al Día, 19, 146–149

    Google Scholar 

  • Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011. MEGA 5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731–2739. DOI: 10.1093/molbev/msr121

    Article  CAS  Google Scholar 

  • Thompson J.D., Higgins D.G., Gibson T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680. DOI: 10.1093/nar/22.22.4673

    Article  CAS  Google Scholar 

  • Umehara A., Kawakami Y., Araki J., Uchida A. 2008. Multiplex PCR for the identification of Anisakis simplex sensu stricto, Anisakis pegreffii and the other anisakid nematodes. Parasitology International, 57, 49–53. DOI: 10.1016/j.parint.2007.08.003

    Article  CAS  Google Scholar 

  • Zerbini A.N., Secchi E.R., Siciliano S., Simões-Lopes P.C. 1997. A review of the occurrence and distribution of whales of the genus Balaenoptera along the brazilian coast. Reports of the International Whaling Commission, 47, 407–417

    Google Scholar 

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Authors and Affiliations

  1. Laboratório de Avaliação e Promoção da Saúde Ambiental, Pós-graduação em Biodiversidade e Saúde, Instituto Oswaldo Cruz, Fiocruz, Av. Brasil, 4365, Rio de Janeiro, Brasil, 21040-360

    Juliana N. Borges, Luiz F. G. Cunha, Daniele F. Miranda & Cláudia P. Santos

  2. Laboratório ECOPESCA - Biologia do Nécton e Ecologia Pesqueira, Departamento e Pósgraduação em Biologia Marinha, UFF, Outeiro de São João Batista s /no, Niterói, Rio de Janeiro, Brasil, 24001-970

    Juliana N. Borges & Cassiano Monteiro-Neto

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  1. Juliana N. Borges
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  2. Luiz F. G. Cunha
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  3. Daniele F. Miranda
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  4. Cassiano Monteiro-Neto
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  5. Cláudia P. Santos
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Correspondence to Juliana N. Borges.

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Borges, J.N., Cunha, L.F.G., Miranda, D.F. et al. Molecular studies on larvae of Pseudoterranova parasite of Trichiurus lepturus Linnaeus, 1758 and Pomatomus saltatrix (Linnaeus, 1766) off Brazilian waters. Acta Parasit. 60, 649–653 (2015). https://doi.org/10.1515/ap-2015-0092

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  • DOI: https://doi.org/10.1515/ap-2015-0092

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