The lack of effectiveness of hyperbaric oxygenation as a treatment for Leishmania major in a mouse model
We aimed to study the effectiveness of hyperbaric oxygen therapy (HOT) (100% oxygen at 2 ATA for 70 minutes each session for 20 consecutive days) on BALB/c male mice infected with Leishmania major. Fifty-one mice were assigned to six groups. Group 1 was treated with HOT from 1 day after the inoculation. In Groups 2–5, treatment began when the cutaneous lesions appeared. Group 2 received HOT only, Group 3 received topical therapy with Leshcutan only, Groups 4 and 5 received a combination of HOT and Leshcutan for 5 and 10 days respectively, and Group 6 did not receive any treatment (control group). When comparing the control group with Group 1, treatment with HOT in Group 1 did not significantly affect the time of the appearance of the lesions. In contrast, mice treated with Leshcutan demonstrated a significant difference in lesion size and spleen dimensions as compared to the rest of the mice (p<0.001). The results show that HOT treatment has no positive effect on the course of Leishmaniasis in a BALB/c mice model infected with Leishmania major. Further studies are needed with a mouse model closer to humans and with different HOT protocols.
KeywordsLeishmania major hyperbaric oxygen therapy mouse model methylbenzethonium chloride paromomycin sulphate
Unable to display preview. Download preview PDF.
- Arana B.A., Mendoza C.E., Rizzo N.R., Kroeger A. 2001. Randomized, controlled, double-blind trial of topical treatment of cutaneous leishmaniasis with paromomycin plus methylbenzethonium chloride ointment in Guatemala. American Journal of Tropical Medicine and Hygiene, 65, 466–470CrossRefGoogle Scholar
- El-On J., Halevy S., Grunwald M.H., Weinrauch L. 1992. Topical treatment of Old World cutaneous leishmaniasis caused by Leishmania major: a double-blind control study. Journal of the American Academy of Dermotology, 27, 22–31Google Scholar
- Giorgio S., Linares E., Ischiropoulos H., Von Zuben F.J., Yamada A., Augusto O. 1998. In vivo formation of electron paramagnetic resonance-detectable nitric oxide and of nitrotyrosine is not impaired during murine leishmaniasis. Infectious Immunology, 66, 807–814Google Scholar
- Kranke P, Bennett M.H., Martyn-St James M., Schnabel A., Debus S.E. 2012. Hyperbaric oxygen therapy for chronic wounds. The Chocrane Datebase of Systematic Reviews, 4, CD004123Google Scholar
- Thom S.R. 2011. Hyperbaric oxygen: its mechanisms and efficacy. Plastic Reconstructive Surgery, 127 Suppl 1, 131S-141SGoogle Scholar