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Biologia

, Volume 72, Issue 2, pp 140–144 | Cite as

Yield improvement of the king oyster mushroom, Pleurotus eryngii, by transformation of its cellulase gene

  • Urarux Romruen
  • Eakaphun BangyeekhunEmail author
Section Cellular and Molecular Biology

Abstract

A plasmid pCAMBIA1301 containing Pleurotus eryngii cellulase gene (PEcbh), under the control of Lentinus edodes glyceraldehyde-3-phosphate dehydrogenase (LEgpd) promoter, was constructed and used as an expression vector. The vector was transformed into the tissue of P. eryngii using Agrobacterium tumefaciens-mediated transformation (ATMT) method and 4 transformants (PET1-4) were obtained. The positive transformants were confirmed by cultivation in media containing hygromycin and by PCR amplification of hygromycin B resistance-LEgpd promoter gene fragment. Unpredicted, cellulase specific activities of the transformants were not higher than those of the wild type. Mushroom cultivation was performed in the laboratory and the results revealed that the average biological efficiency of PET4 was significantly 1.52 times higher than those of the wild type.

Key words

Pleurotus eryngii cellulase Agrobacterium tumefaciens transformation 

Abbreviations

ATMT

Agrobacterium tumefaciens-mediated transformation

BE

biological efficiency

CMC

carboxymethylcellulose

gus

β-glucuronidase

hph

hygromycin B resistance

LEgpd

Lentinus edodes glyceraldehyde-3-phosphate dehydrogenase

PDA

potato dextrose agar

PEcbh

Pleurotus eryngii cellulase gene

PET1-4

Pleurotus eryngii transformant strains 1-4

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Copyright information

© Slovak Academy of Sciences 2017

Authors and Affiliations

  1. 1.Department of Microbiology, Faculty of ScienceSilpakorn UniversityNakhon PathomThailand

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