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Biologia

, Volume 70, Issue 8, pp 1026–1032 | Cite as

Transcriptome analysis for identification of indigo biosynthesis pathway genes in Polygonum tinctorium

  • Yoshiko Minami
  • Bijaya Ketan Sarangi
  • Sanjog Tarachand ThulEmail author
Article

Abstract

Indigo is the most important blue dye for textile dyeing and is biosynthesized in Polygonum tinctorium. Some biochemical studies related to biosynthesis are available. However, genomic and transcriptome studies have not received sufficient attention. Here, we report de novo assembly of transcriptome datasets and its comprehensive analysis. A total of 60,395 unigenes were annotated using BLAST search against the different databases. At least 23,721 unigenes mapped onto different pathways using KEGG database. We found that 3,323 genes are involved in biosynthesis of secondary metabolites, 117 phenylalanine, tyrosine and tryptophan biosynthesis and 147 tryptophan metabolisms. Apart from this, indigo biosynthesis pathway genes viz., dioxygenase, monooxygenase, and glucosyltransferase have also been identified. Fourteen genes encoding cytochrome P450 monooxygenase, 26 glucoside dioxygenase, 9 UDP-glucose D-glucosyltransferase and 52 were β-D-glucosidase. These findings provide a foundation for further analysis of this pathway with potential to enhance the synthesis of indican in P. tinctorium.

Key words

unigenes indican cytochrome P450 monooxygenase UDP-glucosyltransferase 

Abbreviations

COG

clusters of orthologous group

GO

gene ontology

KEGG

Kyoto Encyclopedia of Genes and Genomes

NR

non-redundant protein database

NT

non-redundant nucleotide database

UDP

uridine diphosphate glucose

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Supplementary material

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Transcriptome analysis for identification of indigo biosynthesis pathway genes in Polygonum tinctorium

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Copyright information

© Slovak Academy of Sciences 2015

Authors and Affiliations

  • Yoshiko Minami
    • 1
  • Bijaya Ketan Sarangi
    • 2
  • Sanjog Tarachand Thul
    • 2
    Email author
  1. 1.Department of BiochemistryOkayama University of ScienceJapan
  2. 2.Environmental Biotechnology DivisionCSIR - National Environmental Engineering Research InstituteNagpurIndia

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