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Biologia

, Volume 70, Issue 8, pp 1011–1018 | Cite as

Effects of low-temperature hardening on the biochemical response of winter oilseed rape seedlings inoculated with the spores of Leptosphaeria maculans

  • Katarzyna HuraEmail author
  • Tomasz Hura
  • Marcin Rapacz
  • Agnieszka Płażek
Article

Abstract

The aim of the study was to assess the effects of low-temperature hardening (2°C) on the biochemical compounds and processes that can increase resistance of winter rape to inoculation with Leptosphaeria maculans spores. The study involved an evaluation of the entire pool of phenolic compounds, L-phenylalanine ammonia lyase (PAL) activity, excitation intensity for blue and green fluorescence, catalase (CAT) activity, respiration intensity and heat emission from leaf tissues. All the measurements were performed 24 and 72 hours after the inoculation. Low-temperature hardening, which preceded the inoculation of rape seedlings with spores of L. maculans, caused a significant increase in CAT activity and the level of phenolic compounds. The observed changes in PAL activity reflected the changes in phenolics content. The hardened plants showed a significantly higher intensity of blue fluorescence excitation at 24 and 72 hours after the inoculation, as compared to the non-hardened seedlings. Increased content of phenolic compounds and PAL and catalase activity triggered by the temperature of 2 °C and maintained for 24 hours after the inoculation, may confirm the stimulating effect of the hardening temperature. Intensified emission of blue fluorescence indicating saturation of a cell wall with phenolic compounds makes the cell wall structure less stretchy, more tight and leakproof, and thereby hinders fungal growth through plant tissue.

Key words

cold respiration intensity heat emission catalase phenolics phenylalanine ammonia lyase blue-green fluorescence 

Abbreviations

CAT

catalase

F430

blue fluorescence

F520

green fluorescence

HE

heat emission

LWC

leaf water content

PAL

phenylalanine ammonia lyase

PPFD

photosynthetic photon flux density

Raw4

isolate of L. maculans

RI

respiration intensity

ROS

reactive oxygen species

SPh

soluble phenolics

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Copyright information

© Slovak Academy of Sciences 2015

Authors and Affiliations

  • Katarzyna Hura
    • 1
    Email author
  • Tomasz Hura
    • 2
  • Marcin Rapacz
    • 1
  • Agnieszka Płażek
    • 1
  1. 1.Department of Plant Physiology, Faculty of Agriculture and EconomicsAgricultural UniversityKrakówPoland
  2. 2.The Franciszek Górski Institute of Plant PhysiologyPolish Academy of SciencesKrakówPoland

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