, Volume 70, Issue 8, pp 1063–1069 | Cite as

cDNA cloning, heterologous expression and characterization of a cell wall invertase from copper tolerant population of Elsholtzia haichowensis

  • Chen LiuEmail author
  • Zhongrui Xu
  • Shenwen Cai
  • Luan Zhang
  • Zhiting Xiong


The main objective of the present study was to clone, heterologously express and characterize a novel cell wall invertase (FCWI) from a Cu tolerant population of Elsholtzia haichowensis. The full-length FCWI cDNA contained an open reading frame (ORF) of 1671 bp which encoded a 556-amino-acid protein. The theoretical molecular mass and pI of the deduced protein were 62.5 kDa and 9.29, respectively. Phylogenetic analysis showed that FCWI had a closer evolutionary relationship to cell wall invertase of dicot. FCWI was expressed in methylotrophic yeast Pichia pastoris and purified to near homogeneity. Recombinant FCWI enzyme had pH optima of 4.0 and temperature optima of 50 °C. Activity analyses in the presence of various metal cations indicated that FCWI was completely inhibited by Hg2+ (0%), while retained 77.4% activity when exposure to Cu2+. The Km and Kmax values of FCWI for hydrolyzing sucrose were 0.282 mM and 1.576 μkat/mg, respectively. This is the first report that the heterologous expression and characterization of a cell wall invertase from a Cu tolerant population of E. haichowensis. These results helped to understanding the characteristics of FCWI and its physiological role in the resistance mechanisms of Cu tolerant plants.

Key words

cell wall invertase copper enzyme kinetics Elsholtzia haichowensis heterologous expression Pichia pastoris 


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The work was supported by the National Nature Science Foundation of China (Projects 20677046, 30870365, 31270432, 21477093). The authors declare that there is no conflict of interest.


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Copyright information

© Slovak Academy of Sciences 2015

Authors and Affiliations

  • Chen Liu
    • 1
    Email author
  • Zhongrui Xu
    • 1
  • Shenwen Cai
    • 2
  • Luan Zhang
    • 3
  • Zhiting Xiong
    • 1
  1. 1.School of Resource and Environmental ScienceWuhan UniversityWuhan, HubeiPeople’s Republic of China
  2. 2.College of Resource and EnvironmentZunyi Normal CollegeZunyi, GuizhouPeople’s Republic of China
  3. 3.School of Resource and Environmental ScienceFujian Agriculture and Forestry UniversityFuzhou, FujianPeople’s Republic of China

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