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Biologia

, Volume 70, Issue 5, pp 574–580 | Cite as

Tyrosine phosphorylation mediates starch metabolism in guard cell of Vicia faba

  • Xiao-Mei Qin
  • Ming-Di Bian
  • Zhen-Ming Yang
  • Wu-Liang ShiEmail author
Section Botany

Abstract

Protein tyrosine phosphorylation plays a central role in many signaling pathways leading to cell growth and differentiation in animals. However, the cellular roles for tyrosine phosphorylation in plant remain unclear. Here we describe that tyrosine phosphorylation involved in stomata closing pathway. Genistein, an inhibitor of protein tyrosine kinases (PTKs), could prevent darkness and abscisic acid (ABA)-induced stomatal closure of Vicia faba L. Stomatal closure induced by high external Ca2+ was prevented by phenylarsine oxide (PAO), a specific inhibitor of protein tyrosine phosphatases (PTPs), which promoted closed stomata reopening. Using anti-phosphotyrosine antibody, we detected the distribution of phosphotyrosyl proteins (PYP) by the immuno-fluorescence and immuno-gold electron microscope techniques in guard cells of Vicia faba. It shows that PYP is localized to nucleus, cytoplasm, vacuole and chloroplast of guard cells. Interestingly, the distribution of PYP in chloroplast of guard cell mainly was localized on the surface of starch granule, indicating that there is some relationship between tyrosine phosphorylation and starch metabolism in guard cells. In addition, the starch content in leaves of Vicia faba can be enhanced by inhibiting the activity of PTPs with PAO. Overall, these studies and combined previous report suggest that tyrosine phosphorylation may modulate starch degradation in guard cells, and thus regulating the stomatal movement.

Key words

Vicia faba tyrosine phosphorylation starch metabolism stomatal movement 

Abbreviations

ABA

abscisic acid

BAK1

BRI1-ASSOCIATED KINASE1

BRI1

BRASSINOSTEROID INSENSITIVE 1

DSP

dual specificity protein phosphatase

NO

nitric oxide

PAO

Phenylarsine oxide

PTKs

protein tyrosine kinases

PTPKIS

protein tyrosine phosphatase with a kinase interaction sequence

PTPs

protein tyrosine phosphatases

pY

phospho-tyrosine

PYP

phosphotyrosyl protein

ROS

reactive oxygen species

SEX4

Starch Excess 4.

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Copyright information

© Slovak Academy of Sciences 2015

Authors and Affiliations

  • Xiao-Mei Qin
    • 1
  • Ming-Di Bian
    • 1
  • Zhen-Ming Yang
    • 1
  • Wu-Liang Shi
    • 1
    Email author
  1. 1.Laboratory of Soil and Plant Molecular GeneticsJilin UniversityChangchunPeople’s Republic of China

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