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Acta Parasitologica

, Volume 60, Issue 1, pp 105–111 | Cite as

The in vitro antimalarial interaction of 9-hydroxycalabaxanthone and α-mangostin with mefloquine/artesunate

  • Wanna Chaijaroenkul
  • Kesara Na-BangchangEmail author
Article

Abstract

Multidrug resistance Plasmodium falciparum is the major health problem in Thailand. Discovery and development of new antimalarial drugs with novel modes of action is urgently required. The aim of the present study was to investigate the antimalarial interaction of 9-hydroxycalabaxanthone and α-mangostin with the standard antimalarial drugs mefloquine and artesunate in chloroquine sensitive (3D7) and chloroquine resistant (K1) P. falciparum clones in vitro. Median (range) IC50 (drug concentration which produces 50% parasite growth inhibition) values of the 9-hydroxycalabaxanthone, α-mangostin, artesunate and mefloquine for 3D7 vs K1 clones were 1.5 (0.9–2.1) vs 1.2 (1.1–1.6) μM, 17.9 (15.7.0–20.0) vs 9.7 (6.0–14.0) μM, 1.0 (0.4–3.0) vs 1.7 (1.0–2.5) nM, and 13.3 (11.1–13.3) vs 7.1 (6.7–12.2) nM, respectively. Analysis of isobologram and combination index (CI) of 9-hydroxycalabaxanthone with artesunate or mefloquine showed synergistic and indifference antimalarial interaction, respectively. α-mangostin-artesunate combination exhibited a slight antagonistic effect of antimalarial interaction, whereas α-mangostin and mefloquine combination showed indifference interaction in both clones. The combination of 9-hydroxycalabaxanthone with α-mangostin showed the synergistic antimalarial interaction in both clones.

Keywords

Multidrug resistance Plasmodium falciparum 9-hydroxycalabaxanthone α-mangostin drug combination 

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Copyright information

© W. Stefański Institute of Parasitology, PAS 2015

Authors and Affiliations

  1. 1.Center of Excellence in Molecular Biology and Pharmacology of Malaria and Cholangiocarcinoma, Chulabhorn International College of MedicineThammasat UniversityPatumthaniThailand

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