Acta Parasitologica

, Volume 60, Issue 1, pp 21–25 | Cite as

Molecular detection of Dirofilaria immitis, Hepatozoon canis, Babesia spp., Anaplasma platys, and Ehrlichia canis in dogs on Costa Rica

  • Lanjing Wei
  • Patrick Kelly
  • Kate Ackerson
  • Heba S. El-Mahallawy
  • Bernhard Kaltenboeck
  • Chengming WangEmail author


Although vector-borne diseases are important causes of morbidity and mortality in dogs in tropical areas, there is little information on these conditions in Costa Rica. In PCRs of blood from dogs in Costa Rica, we did not detect DNAs of Rickettsia (R.) felis and Coxiella (C.) burnetii but we did find evidence of infection with Dirofilaria (D.) immitis (9/40, 22.5%), Hepatozoon (H.) canis (15/40, 37.5%), Babesia spp. (10/40, 25%; 2 with B. gibsoni and 8 with B. vogeli), Anaplasma (A.) platys (3/40, 7.5%) and Ehrlichia (E.) canis (20/40, 50%). Nine dogs (22.5%) were free of any vector-borne pathogens while 14 (35%) were infected with a single pathogen, 11 (27.5%) with two, 4 (10%) with three, 1 (2.5%) with four, and 1 (2.5%) with five pathogens. Dogs in Costa Rica are commonly infected with vector-borne agents.


Costa Rica Dirofilaria immitis Babesia gibsoni/vogeli Anaplasma platys Ehrlichia canis Hepatozoon canis 


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  1. Abdad M., Stenos J., Graves S. 2011. Rickettsia felis, an emerging flea-transmitted human pathogen. Emerging Health Threats Journal, 4, 7168. DOI: 10.3402/ehtj.v4i0.7168CrossRefGoogle Scholar
  2. Alleman A.R., Wamsley H.L. 2008. An update on anaplasmosis in dogs. Veterinary Medicine, 103, 212–220Google Scholar
  3. Ayoob A.L., Hackner S.G., Prittie J. 2010. Clinical management of canine babesiosis. Journal of Veterinary Emerging Critical Care (San Antonio), 20, 77–89. DOI: 10.1111/j.1476-4431.2009.00489.xCrossRefGoogle Scholar
  4. Baneth G., Samish M., Shkap V. 2007. Life cycle of Hepatozoon canis (Apicomplexa: Adeleorina: Hepatozoidae) in the tick Rhipicephalus sanguineus and domestic dog (Canis familiaris). Journal of Parasitology, 93, 283–299. DOI: 10.1645/GE-494R.1CrossRefGoogle Scholar
  5. Baneth G. 2011. Perspectives on canine and feline hepatozoonosis. Veterinary Parasitology. 181, 3–11. DOI: 10.1016/j.vetpar.2011.04.015CrossRefGoogle Scholar
  6. Bermudez C.S., Zaldívar A.Y., Spolidorio M.G., Moraes-Filho J., Miranda R.J., Caballero C.M., Mendoza Y., Labruna M.B. 2011. Rickettsial infection in domestic mammals and their ectoparasites in El Valle de Antón, Coclé, Panamá. Veterinary Parasitology, 177, 134–138. DOI: 10.1016/j.vetpar.2010.11.020CrossRefGoogle Scholar
  7. Berri M., Rekiki A., Boumedine K.S., Rodolakis A. 2009. Simultaneous differential detection of Chlamydophila abortus, Chlamydophila pecorum and Coxiella burnetii from aborted ruminant’s clinical samples using multiplex PCR. BMC Microbiology, 9, 130. DOI: 10.1186/1471-2180-9-130CrossRefGoogle Scholar
  8. Brown G.K., Canfield P.J., Dunstan R.H., Roberts T.K., Martin A.R., Brown C.S., Irving R. 2006. Detection of Anaplasma platys and Babesia vogeli and their impact on platelet numbers in free-roaming dogs associated with remote Aboriginal communities in Australia. Australian Veterinary Journal, 84, 321–325. DOI: 10.1111/j.1751-0813.2006.00029.x ai]Buhariwalla F., Cann B., Marrie T.J. 1996. A dog-related outbreak of Q fever. Clinical Infectious Diseases, 23, 753–755. DOI: 10.1093/clinids/23.4.753CrossRefGoogle Scholar
  9. Cuervo P.F., Mera Y., Sierra R., Waisman V., Gerbeno L., Sidoti L., Albonico F., Mariconti M., Mortarino M., Pepe P., Cringoli G., Genchi C., Rinaldi L. 2013. Detection of Dirofilaria immitis in mid-western arid Argentina. Acta Parasitologica, 58, 612–614. DOI: 10.2478/s11686-013-0177-zCrossRefGoogle Scholar
  10. Cutler S.J., Bouzid M., Cutler R.R. 2007. Q fever. Journal of Infection, 54, 313–318. DOI: 10.1016/j.jinf.2006.10.048CrossRefGoogle Scholar
  11. Dantas-Torres F. 2008. The brown dog tick, Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae): from taxonomy to control. Veterinary Parasitology, 152, 173–185. DOI: 10.1016/j.vetpar.2007.12.030CrossRefGoogle Scholar
  12. Esch K.J., Petersen C.A. 2013. Transmission and epidemiology of zoonotic protozoal diseases of companion animals. Clinical Microbiology Reviews, 26, 58–85. DOI: 10.1128/CMR.00067-12CrossRefGoogle Scholar
  13. Herrer A., Christensen H.A. 1976. Natural cutaneous leishmaniasis among dogs in Panama. American Journal of Tropical Medicine and Hygiene, 25, 59–63. DOI: 10.1590/S0074-02762011000800021CrossRefGoogle Scholar
  14. Hii S.F., Abdad M.Y., Kopp S.R., Stenos J., Rees R.L., Traub R.J. 2013. Seroprevalence and risk factors for Rickettsia felis exposure in dogs from Southeast Queensland and the Northern Territory, Australia. Parasites & Vectors, 6, 159. DOI: 10.1186/1756-3305-6-159CrossRefGoogle Scholar
  15. Hun L., Troyo A., Taylor L., Barbieri A.M., Labruna M.B. 2011. First report of the isolation and molecular characterization of Rickettsia amblyommii and Rickettsia felis in Central America. Vector-Borne Zoonotic Diseases, 11, 1395–1397. DOI: 10.1089/vbz.2011.0641CrossRefGoogle Scholar
  16. Iguchi A., Matsuu A., Fujii Y., Ikadai H., Hikasa Y. 2013. The in vitro interactions and in vivo efficacy of atovaquone and proguanil against Babesia gibsoni infection in dogs. Veterinary Parasitology. 197, 527–533. DOI: 10.1016/j.vetpar.2013.06.006CrossRefGoogle Scholar
  17. Kelly P.J., Xu C., Lucas H., Loftis A., Abete J., Zeoli F., Stevens A., Jaegersen K., Ackerson K., Gessner A., Kaltenboeck B., Wang C. 2013. Ehrlichiosis, babesiosis, anaplasmosis and hepatozoonosis in dogs from St. Kitts, West Indies. PLoS One, 8, e53450. DOI: 10.1371/journal.pone.0053450CrossRefGoogle Scholar
  18. Li Y., Wang C., Allen K.E., Little S.E., Ahluwalia S.K., Gao D., Macintire D.K., Blagburn B.L., Kaltenboeck B. 2008. Diagnosis of canine Hepatozoon spp. infection by quantitative PCR. Veterinary Parasitology, 157, 50–58. DOI: 10.1016/j.vetpar.2008.06.027CrossRefGoogle Scholar
  19. Maggi R.G., Mascarelli P.E., Havenga L.N., Naidoo V., Breitschwerdt E.B. 2013. Co-infection with Anaplasma platys, Bartonella henselae and Candidatus Mycoplasma haematoparvum in a veterinarian. Parasites & Vectors, 6, 103. DOI: 10.1186/1756-3305-6-103CrossRefGoogle Scholar
  20. Matsuu A., Kawabe A., Koshida Y., Ikadai H., Okano S., Higuchi S. 2004. Incidence of canine Babesia gibsoni infection and subclinical infection among Tosa dogs in Aomori Prefecture, Japan. Journal of Veterinary Medical Science, 66, 893–897. DOI: 10.1292/jvms.66.893CrossRefGoogle Scholar
  21. Matthewman L.A., Kelly P.J., Bobade P.A., Tagwira M., Mason P.R., Majok A., Brouqui P., Raoult D. 1993. Infections with Babesia canis and Ehrlichia canis in dogs in Zimbabwe. Veterinary Record, 133, 344–346. DOI: 10.1136/vr.133.14.344CrossRefGoogle Scholar
  22. McCall J.W., Genchi C., Kramer L.H., Guerrero J., Venco L. 2008 Heartworm disease in animals and humans. Advances in Parasitology, 66, 193–285. DOI: 10.1016/S0065-308X(08)00204-2CrossRefGoogle Scholar
  23. Perez M., Bodor M., Zhang C., Xiong Q., Rikihisa Y. 2006. Human infection with Ehrlichia canis accompanied by clinical signs in Venezuela. Annals of the New York Academy of Sciences, 1078, 110–117. DOI: 10.1196/annals.1374.016CrossRefGoogle Scholar
  24. Pineda V., Saldaña A., Monfante I., Santamaría A., Gottdenker N.L., Yabsley M.J., Rapoport G., Calzada J.E. 2011. Prevalence of trypanosome infections in dogs from Chagas disease endemic regions in Panama, Central America. Veterinary Parasitology, 178, 360–363. DOI: 10.1016/j.vetpar.2010.12.043CrossRefGoogle Scholar
  25. Rojas A., Rojas D., Montenegro V., Gutiérrez R., Yasur-Landau D., Banet,h G. 2014. Vector-borne pathogens in dogs from Costa Rica: First molecular description of Babesia vogeli and Hepatozoon canis infections with a high prevalence of monocytic ehrlichiosis and the manifestations of co-infection. Veterinary Parasitology, 199, 121–128. DOI: 10.1016/j.vetpar.2013.10.027CrossRefGoogle Scholar
  26. Romero L.E., Meneses A.I., Salazar L., Jiménez M., Romero J.J., Aguiar D.M., Labruna M.B., Dolz G. 2011. First isolation and molecular char-acterization of Ehrlichia canis in Costa Rica, Central America. Research in Veterinary Science, 91, 95–97. DOI: 10.1016/j.rvsc.2010.07.021CrossRefGoogle Scholar
  27. Ryan P.R., Arana B.A., Ryan J.R., Wirtz R.A., Wortmann G.W., Rizzo N.R. 2003. The domestic dog, a potential reservoir for Leishmania in the Peten region of Guatemala. Veterinary Parasitology, 115, 1–7. DOI: 10.1016/S0304-4017(03)00158-4CrossRefGoogle Scholar
  28. Scorza A.V., Duncan C., Miles L., Lappin M.R. 2011. Prevalence of selected zoonotic and vector-borne agents in dogs and cats in Costa Rica. Veterinary Parasitology, 183, 178–183. DOI: 10.1016/j.vetpar.2011.06.025CrossRefGoogle Scholar
  29. Shaw S.E., Day M.J., Birtles R.J., Breitschwerdt E.B. 2001. Tickborne infectious diseases of dogs. Trends in Parasitology, 17, 74–80. DOI: 10.1016/S1471-4922(00)01856-0CrossRefGoogle Scholar
  30. Simón F., López-Belmonte J., Marcos-Atxutegi C., Morchón R., Martín-Pacho J.R., What is happening outside North America regarding human dirofilariasis? Veterinary Parasitology, 133, 181–189. DOI: 10.1016/j.vetpar.2005.03.033Google Scholar
  31. Solano-Gallego L., Baneth G. 2011. Babesiosis in dogs and catsexpanding parasitological and clinical spectra. Veterinary Parasitology, 181, 48–60. DOI: 10.1016/j.vetpar.2011.04.023CrossRefGoogle Scholar
  32. Taboada J., Lobetti R. 2006. Babesiosis. In: Greene CE, editor. Infectious Diseases of the Dog and Cat. Saunders Elsevier. Missouri. 722–736Google Scholar
  33. Troyo A., Álvarez D., Taylor L., Abdalla G., Calderón-Arguedas Ó., Zambrano M.L., Dasch G.A., Lindblade K., Hun L., Eremeeva M.E., Estévez A. 2012. Rickettsia felis in Ctenocephalides felis from Guatemala and Costa Rica. American Journal of Tropical Medicine and Hygiene, 86, 1054–1056. DOI: 10.4269/ajtmh.2012.11-0742CrossRefGoogle Scholar
  34. Wang C., Ahluwalia S.K., Li Y., Gao D., Poudel A., Chowdhury E., Boudreaux M.K., Kaltenboeck B. 2010. Frequency and therapy monitoring of canine Babesia spp. infection by high-resolution melting curve quantitative FRET-PCR. Veterinary Parasitology, 168, 11–18. DOI: 10.1016/j.vetpar.2009.10.015CrossRefGoogle Scholar
  35. Wang C., Mount J., Butler J., Gao D., Jung E., Blagburn B.L., Kaltenboeck B. 2012. Real-time PCR of the mammalian hydroxymethylbilane synthase (HMBS) gene for analysis of flea (Ctenocephalides felis) feeding patterns on dogs. Parasites & Vectors, 5, 4. DOI: 10.1186/1756-3305-5-4CrossRefGoogle Scholar
  36. Wei L., Kelly P., Ackerson K., Zhang J., El-Mahallawy HS., Kaltenboeck B., Wang C. 2014. First report of Babesia gibsoni in Central America and survey for vector-borne infections in dogs from Nicaragua. Parasites & Vectors, 7, 126. DOI: 10.1186/1756-3305-7-126CrossRefGoogle Scholar
  37. Zhang J., Wei L., Kelly P., Freeman M., Jaegerson K., Gong J., Xu B., Pan Z., Xu C., Wang C. 2013. Detection of Salmonella spp. using a generic and differential FRET-PCR. PLoS One, 8, e76053. DOI: 10.1371/journal.pone.0076053CrossRefGoogle Scholar

Copyright information

© W. Stefański Institute of Parasitology, PAS 2015

Authors and Affiliations

  • Lanjing Wei
    • 1
  • Patrick Kelly
    • 2
  • Kate Ackerson
    • 2
  • Heba S. El-Mahallawy
    • 1
    • 3
  • Bernhard Kaltenboeck
    • 4
  • Chengming Wang
    • 1
    Email author
  1. 1.Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and ZoonosesYangzhou University College of Veterinary MedicineYangzhou, JiangsuP.R. China
  2. 2.Ross University School of Veterinary MedicineBasseterre, St.KittsIndia
  3. 3.Faculty of Veterinary MedicineSuez Canal UniversityIsmailiaEgypt
  4. 4.Auburn University School of Veterinary MedicineAuburnUSA

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