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Hormones

, Volume 13, Issue 4, pp 532–542 | Cite as

Short-chain fatty acids increase expression and secretion of stromal cell-derived factor-1 in mouse and human pre-adipocytes

  • Venkat N. Vangaveti
  • Catherine Rush
  • Linda Thomas
  • Roy R. Rasalam
  • Usman H. Malabu
  • Scott G. McCoombe
  • Richard L. Kennedy
Research paper

Abstract

OBJECTIVE

Stromal cell-derived factor-1 (SDF-1) is expressed in pre-adipocytes but its role is unknown. We investigated butyrate (a histone deacetylase inhibitor — HDACi) and other short-chain fatty acids (SCFA) in the regulation of SDF-1. We further investigated whether effects of SCFA were signalled through G protein-coupled receptors FFA2 and FFA3.

DESIGN AND RESULTS

SDF-1 mRNA expression and protein secretion were studied in 3T3-L1 cells and human pre-adipocytes. SDF-1 was abundant, with mRNA and protein levels increased by butyrate. This was replicated with acetate and propionate, but not with trichostatin or valproate. Trichostatin inhibited SDF-1 secretion. Pertussis toxin blocked stimulation by butyrate. The order of potency of SCFA in stimulating SDF-1 (C3 > C4 > C2) is consistent with action through FFA3. Silencing the FFA3 gene abolished butyrate-stimulated SDF-1 expression and secretion. FFA3 was expressed in both pre-adipocytes and adipocytes, while FFA2 was expressed in adipocytes only. SDF-1 expression was low in murine macrophage J774.2 cells, while the SDF-1 receptor CXCR4 was absent from 3T3-L1 cells but abundant in J774.2 macrophages. In human pre-adipocytes, FFA3 was also expressed and SCFA increased SDF-1 secretion.

CONCLUSIONS

SDF-1 and CXCR4 may mediate the interaction between adipose stromal cells and macrophages. Effects of SCFA are mediated through FFA3, but not histone deacetylase inhibition.

Key Words

Butyrate Fatty acids FFA2 FFA3 Pre-adipocytes Propionate 

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Copyright information

© Hellenic Endocrine Society 2014

Authors and Affiliations

  • Venkat N. Vangaveti
    • 1
  • Catherine Rush
    • 2
  • Linda Thomas
    • 1
  • Roy R. Rasalam
    • 1
  • Usman H. Malabu
    • 1
  • Scott G. McCoombe
    • 3
  • Richard L. Kennedy
    • 3
  1. 1.School of Medicine and DentistryJames Cook UniversityDouglasAustralia
  2. 2.School of Veterinary and Biomedical SciencesJames Cook UniversityDouglasAustralia
  3. 3.School of MedicineDeakin UniversityGeelongAustralia

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