Abstract
In cancer, spontaneous apoptosis of circulating peripheral blood lymphocytes (PBLs) is a general phenomenon. In this study we aimed to determine whether spontaneous apoptosis of circulating PBLs of patients with malignant melanoma occurs. Pathologically proven 45 patients with malignant melanoma and 19 healthy controls were included in this study. Samples were obtained both on first admission before treatment, either adjuvant or metastatic, and follow-up period of patients. Human active caspase-3 immunoassay (R&D Systems, Inc. MN, USA) employs the quantitative sandwich enzyme immunoassay technique. A monoclonal specific for caspase-3 has been used. The spontaneously apoptotic PBLs in melanoma patients were not significantly different from those obtained for normal controls (p=0.25). None of the clinical characteristics were significantly correlated with spontaneous apoptosis (p>0.05). Likewise, we found that apoptosis in PBLs was not a prognostic factor in melanoma patients (p=0.79). In conclusion, we did not observe accelerated apoptosis of PBLs in patients with melanoma. Further studies are necessary to determine the potential prognostic importance of this observation.
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Saito T, Dworacki G, Gooding W, Lotze MT, Whiteside TL. Spontaneous apoptosis of CD8+ T lymphocytes in peripheral blood of patients with advanced melanoma. Clin Cancer Res 2000;6:1351–1364.
Miescher S, Whiteside T. L, Von Carrell S, Fliedner V. Functional properties of tumor-infiltrating and blood lymphocytes in patients with solid tumors: effects of tumor cells and their supernatants on proliferative response of lymphocytes. J Immunol 1986;136:1899–1907.
Takahashi A, et al. Elevated caspase-3 activity in peripheral blood T cells coexists with increased degree of T-cell apoptosis and down-regulation of TCR zeta molecules in patients with gastric cancer. Clin Cancer Res 2001;7:74–80.
Rabinowich H, et al. Lymphocyte apoptosis induced by Fas ligand-expressing ovarian carcinoma cells. J Clin Invest 1998;101:2579–2788.
Gastman BR, Johnson DE, Whiteside TL, Rabinowich H. Caspase-mediated degradation of T-cell receptor ζ-chain. Cancer Res 1999;59:1422–1427.
Saito T, et al. Spontaneous ex vivo apoptosis of peripheral blood mononuclear cells in patients with bead and neck cancer. Clin Cancer Res 1999;5:1263–1273.
Mese H, Sasaki A, Nakayama S, Alcalde RE, Matsumura T. The role of caspase family protease, caspase-3 on cisplatin-induced apoptosis in cisplatin-resistant A431 cell line. Cancer Chemother Pharmacol 2000;46:241–245.
Elstrom RL, Thompson CB, Cell life and cell death: In Abeloff MD, Armitage JO, Niederhuber JE, Kastan MB, and McKenna WG (eds). Clinical Oncology, 3rd ed. Elsevier Churchill Livingstone, Philadelphia, PA, 2004, pp. 101–112.
Hoffmann TK, et al. Spontaneous apoptosis of circulating T lymphocytes in patients with head and neck cancer and its clinical importance. Clin Cancer Res 2002;8:2553–2562.
Reichert TE, Strauss L, Wagner EM, Gooding W, Whiteside TL. Signaling abnormalities, apoptosis, and reduced proliferation of circulating and tumor-infiltrating lymphocytes in patients with oral carcinoma. Clin Cancer Res 2002;8:3137–3145.
Bengtsson AA, Sturfelt G, Gullstrand B, Truedsson L. Induction of apoptosis in monocytes and lymphocytes by serum from patients with systemic lupus erythematosus-an additional mechanism to increased autoantigen load? Clin Exp Immunol 2004;135:535–543.
Pasqui AL, et al. Lymphocyte apoptosis in non-ST segment elevation acute myocardial infarction. Ann Ital Med Int 2003;18:154–161.
Le Tulzo Y, et al. Early circulating lymphocyte apoptosis in human sepctic shock is associated with poor outcome. Shock 2002;18:487–494.
Delogu G, et al. Apoptosis and surgical trauma: dysregulated expression of death and survival factors on peripheral lymphocytes. Arch Surg 2000;135:1141–1147.
Roe MF, et al. Lymphcyte apoptosis in acute respiratory syncytial virus bronchiolitis. Clin Exp Immunol 2004;137:139–145.
Zachwieja J, et al. Increased apoptosis of peripheral blood lymphocytes in children with nephrotic syndrome. Pediatr Nephrol 2002;17:197–200.
Fan YM, Zeng WJ, Wu ZH, Li SF. Immunophenotypes, apoptosis, and expression of Fas and Bcl-2 from peripheral blood lymphocytes in patients with secondary early syphilis. Sex Transm Dis 2004;31:221–224.
Simao TA, et al. Detection and analysis of apoptosis in peripheral blood cells from breast cancer patients. Braz J Med Biol Res 1999;32:403–406.
Gastman BR, Johnson DE, Whiteside TL, Rabinowich H. Tumor-induced apoptosis of T lymphocytes: elucidation of intracellular apoptotic events. Blood 2000;95:2015–2023.
Sun B, Zhang J, Wang B. Expression of caspase3 gene does not correlate with tumor cell apoptosis in primary hepatocellular carcinoma. Zhonghua Zhong Liu Za Zhi 2000;22:39–41.
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Tas, F., Duranyildiz, D., Argon, A. et al. Apoptosis of lymphocytes in peripheral blood of patients with melanoma. Med Oncol 22, 177–181 (2005). https://doi.org/10.1385/MO:22:2:177
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DOI: https://doi.org/10.1385/MO:22:2:177