Medical Oncology

, Volume 20, Issue 2, pp 147–155 | Cite as

Laminin-5 γ2-chain expression and DNA ploidy as predictors of prognosis in endometrial carcinoma

  • C. Lundgren
  • B. Frankendal
  • C. Silfverswärd
  • B. Nilsson
  • K. Tryggvason
  • G. Auer
  • B. Nordström
Original Article

Abstract

Expression of the laminin-5 γ2-chain in carcinoma cells has been implicated in tumor invasion. The aim was to investigate the expression and prognostic significance of the In-5 γ2-chain compared with clinicopathological factors and tumor cell DNA ploidy in endometrial carcinoma. Histological specimens from 80 endometrial carcinomas were examined with respect to immunohistochemical In-5 γ2-chain expression and correlated to the clinicopathological characteristics, DNA ploidy, and survival. Sixty-eight of 80 investigated cases were judged to be positive for the In-5 γ2-chain. Ln-5 γ2-chain did not show any correlation to stage, histopathological subtype, grade, and DNA ploidy. In univariate analyses, advanced stage (p<0.001), nonendometrioid carcinoma (p=0.030), low grade (p<0.001), aneuploid tumors (p<0.001), and In-5 γ2-chain expression (p=0.017) were highly associated with poor survival. Aneuploid tumors in combination with strong In-5 γ2-chain expression were significant predictors (p<0.001) of poor prognosis. In multivariate analyses including stage, histopathological subgroup, grade, DNA ploidy, and In-5 γ2-chain expression, all lost their significant prognostic information except for stage (p<0.001) and grade (p<0.05). Ln-5 γ2-chain expression and DNA ploidy both as a single parameter and in combination were demonstrated to be significant prognostic factors in univariate analysis. However, stage and grade provided more useful clinical information beyond histopathological subgroup, DNA ploidy, and In-5 γ2-chain expression. The results also indicate that In-5 γ2-chain expression is upregulated during the progression of endometrial carcinoma.

Key Words

Endometrial carcinoma laminin-5 γ2 chain DNA ploidy prognosis basement membranes 

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Copyright information

© Humana Press Inc 2003

Authors and Affiliations

  • C. Lundgren
    • 1
  • B. Frankendal
    • 1
  • C. Silfverswärd
    • 2
  • B. Nilsson
    • 3
  • K. Tryggvason
    • 4
  • G. Auer
    • 2
  • B. Nordström
    • 1
  1. 1.Department of Gynaecologic OncologyRadiumhemmet, Karolinska HospitalStockholmSweden
  2. 2.Department of Pathology and Cell AnalysisMedical Biochemistry and Biophysics, Karolinska Hospital and InstituteStockholmSweden
  3. 3.Department of Cancer EpidemiologyMedical Biochemistry and Biophysics, Karolinska Hospital and InstituteStockholmSweden
  4. 4.Department of Matrix BiologyMedical Biochemistry and Biophysics, Karolinska Hospital and InstituteStockholmSweden

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