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Evidence of DNA rearrangements in the 128-kilobase pBtoxis plasmid of Bacillus thuringiensis israelensis

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Abstract

BUPM97 is a novel Tunisian isolate of Bacillus thuringiensis israelensis presenting insecticidal activity against Culex pipiens larvae. The δ-endotoxins pattern of this strain was different from that of the reference strain B. thuringiensis israelensis H14. Therefore, the study of its cry genes content was carried out by restriction-fragment-length-polymorphism (RFLP) using specific cry genes probes and by DNA sequencing. It was clearly demonstrated that in the strain BUPM97 the cry4A and cry10A genes were deleted from the B. thuringiensis israelensis 128-kb pBtoxis plasmid. In addition, a strong DNA sequence polymorphism was evidenced in the same plasmid downstream from the cry4B gene. This very particular DNA dynamic evidenced in this new strain of B. thuringiensis israelensis should be taken into consideration, regarding the strain stability during the industrial production of B. thuringiensis bioinsecticides.

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Authors and Affiliations

  1. Laboratory of Biopesticides, Centre of Biotechnology of Sfax, P.O. Box ‘K’, 3038, Sfax, Tunisia

    Raida Zribi Zghal & Samir Jaoua

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  1. Raida Zribi Zghal
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  2. Samir Jaoua
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Correspondence to Samir Jaoua.

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Zghal, R.Z., Jaoua, S. Evidence of DNA rearrangements in the 128-kilobase pBtoxis plasmid of Bacillus thuringiensis israelensis . Mol Biotechnol 33, 191–198 (2006). https://doi.org/10.1385/MB:33:3:191

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