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The use of hydrolysis and hairpin probes in real-time PCR

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Abstract

In this review we discuss the limitations of traditional PCR methods and introduce real-time PCR, a fluorescence-based detection system, as a method that overcomes these limitations. We describe the technologies involved in real-time PCR including the design of hydrolysis and hairpin probes and the practical applications of such systems.

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Authors and Affiliations

  1. Department of Microbiology, House on the Hill, Stobhill Hospital, North Glasgow University Hospital NHS Trust, Balornock Road, G21 3UW, Glasgow

    Susan M. McChlery & Stuart C. Clarke

  2. Faculty of Biomedical and Life Sciences, University of Glasgow, G12 8QQ, UK

    Stuart C. Clarke

Authors
  1. Susan M. McChlery
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  2. Stuart C. Clarke
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Correspondence to Stuart C. Clarke.

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McChlery, S.M., Clarke, S.C. The use of hydrolysis and hairpin probes in real-time PCR. Mol Biotechnol 25, 267–273 (2003). https://doi.org/10.1385/MB:25:3:267

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