Skip to main content
SpringerLink
Log in

Differential gene expression during somatic embryogenesis in Coffea arabica L., revealed by RT-PCR differential display

  • Research
  • Published:
Molecular Biotechnology Aims and scope Submit manuscript

Abstract

Molecular and biochemical studies of somatic embryogenesis may help to shed light on the mechanisms governing this phenomenon. In this article, a differential display analysis approach was employed to investigate the changes taking place during the induction of somatic embryogenesis in leaf explants and suspension cultures of coffee. Cloned fragments show homologies to several proteins reported in databases, but only one has previously been described as regulated during somatic embryogenesis. By a reverse dot blot modification, the expression pattern of such fragments was evaluated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Zimmerman, J. L. (1993) Somatic embryogenesis: a model for early development in higher plants. The Plant Cell 5, 1411–1423.

    Article  PubMed  Google Scholar 

  2. Mordhorst, A. P., Toonen, M. A. J. and De Vries, S. C., (1997) Plant embryogenesis. Crit. Rev. Plant Sci. 16, 535–576.

    Article  Google Scholar 

  3. Sung, Z. R. and Okimoto, R., (1981) Embryonic proteins in somatic embryos of carrot. Proc. Natl. Acad. Sci USA 78, 3683–3687.

    Article  PubMed  CAS  Google Scholar 

  4. Dudits, D., Györgyey, J., Bögre, L. and Bakó, L. (1995) Molecular biology of somatic embryogenesis, in: In vitro embryogenesis in plants (Thorpe, T. A., ed.), Kluwer Academic Publishers, Dordrecht, pp. 267–308.

    Google Scholar 

  5. Choi, J. H., Liu, L.-S., Borkird, C. and Sung, Z. R., (1987) Cloning of genes developmentally regulated during plant embryogenesis. Proc. Natl. Acad. Sci. USA 84, 1906–1910.

    Article  PubMed  CAS  Google Scholar 

  6. Wilde, H. D., Nelson, W. S., Booij, H., De Vries, S. C. and Thomas, T. L. (1988) Gene-expression programs in embryogenic and non-embryogenic carrot cultures. Planta 176, 205–211.

    Article  CAS  Google Scholar 

  7. Kawahara, R. and Komamine, A. (1995) Molecular basis of somatic embryogenesis, in: Biotechnology in Agriculture and Forestry. Vol. 30. Somatic embryogenesis and synthetic seed I (Bajaj, Y. P. S., ed.), Springer-Verlag, Berlin, pp. 30–40.

    Google Scholar 

  8. Aleith, F. and Richter, G., (1990) Gene expression during induction of somatic embryogenesis in carrot cell suspensions. Planta 183, 17–24.

    Google Scholar 

  9. Thomas, T. L. (1993) Gene expression during plant embryogenesis and germination: An overview. Plant Cell 5, 1401–1410.

    Article  PubMed  CAS  Google Scholar 

  10. Wilde, H. D., Seffens, W. S. and Thomas, T. L. (1995) Gene expression in somatic embryos, in: Biotechnology in agriculture and forestry. Vol. 30. Somatic embryogenesis and synthetic seed I (Bajaj, Y. P. S., ed.), Springer-Verlag, Berlin, pp. 41–52.

    Google Scholar 

  11. Dodeman, V. L., Ducreux, G. and Kreis, M. (1997) Zygotic embryogenesis versus somatic embryogenesis. J. Exp. Bot. 48, 1493–1509.

    Google Scholar 

  12. Lin, X. Y., Hwang, G. J. H. and Zimmerman, J. L., (1996) Isolation and characterization of a diverse set of genes from carrot somatic embryos. Plant Physiol. 112, 1365–1374.

    Article  PubMed  CAS  Google Scholar 

  13. Dong, J. Z. and Dunstan, D. I., (1996) Expression of abundant mRNAs during somatic embryogenesis of white spruce [Picea glauca (Moench) Voss]. Planta 199, 459–466.

    Article  PubMed  CAS  Google Scholar 

  14. Giroux, R. W. and Pauls, K. P., (1997) Characterization of somatic embryogenesis-related cDNAs from alfalfa (Medicago sativa L). Plant Mol. Biol. 33, 393–404.

    Article  PubMed  CAS  Google Scholar 

  15. Fowler, M. R., Ong, L. M., Russinova, E., Atanassov, A. I., Scott, N. W., Slater, A. and Elliott, M. C., (1998) Early changes in gene expression during direct somatic embryogenesis in alfalfa revealed by RAP-PCR. J. Exp. Bot. 49, 249–253.

    Article  CAS  Google Scholar 

  16. Staritsky, G., (1970) Embryoid formation in callus tissues of coffee. Acta Bot. Need. 19, 509–514.

    Google Scholar 

  17. Söndahl, M. R. and Sharp, W. R., (1977) High frequency induction of somatic embryos in cultured leaf explants of Coffea arabica L. Z. Pflanzenphysiol. 81, 395–408.

    Google Scholar 

  18. Yasuda, T., Fujii, Y. and Yamaguchi, T., (1985) Embryogenic callus induction from Coffea arabica leaf explants by benzyladenine. Plant Cell Physiol. 26, 595–597.

    CAS  Google Scholar 

  19. Quiroz-Figueroa, F. R., Fuentes-Cerda, C. F. J., Rojas-Herrera, R., Loyola-Vargas, V. M. (2002): Histological studies on ontogenesis, development stages and differentiation of two different somatic embryogenesis systems of Coffea arabica. To be published.

  20. Murashige, T. and Skoog, F., (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.

    Article  CAS  Google Scholar 

  21. Chen, W., Hardy, P. and Wilce, P. A., (1997) Differential expression of mitochondrial NADH dehydrogenase in ethanol-treated rat brain: Revealed by differential display. Alcohol. Clin. Exp. Res. 21, 1053–1056.

    PubMed  CAS  Google Scholar 

  22. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1980) Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

    Google Scholar 

  23. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. and Lipman, D. J., (1990) Basic local alignment search tool. J. Mol. Biol. 215, 403–410.

    PubMed  CAS  Google Scholar 

  24. Jeanmougin, F., Thompson, J. D., Gouy, M., Higgins, D. G. and Gibson, T. J., (1998) Multiple sequence alignment with Clustal X. Trends Biochem. Sci. 23, 403–405.

    Article  PubMed  CAS  Google Scholar 

  25. Liang, P. and Pardee, A. B., (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257, 967–971.

    Article  PubMed  CAS  Google Scholar 

  26. Appel, M., Bellstedt, D. U. and Gresshoff, P. M., (1999) Differential display of eukaryotic mRNA: Meeting the demands of the new millennium? J. Plant Physiol. 154, 561–570.

    CAS  Google Scholar 

  27. Stals, H., Casteels, M. P., Van Montagu, M. and Inzé, D., (2000) Regulation of cyclin-dependent kinases in Arabidopsis thaliana. Plant Mol. Biol. 43, 583–593.

    Article  PubMed  CAS  Google Scholar 

  28. Waters, E. R., Lee, G. J. and Vierling, E., (1996) Evolution, structure and function of the small heat shock proteins in plants. J. Exp. Bot. 47, 325–338.

    Article  CAS  Google Scholar 

  29. Zimmerman, J. L., Apuya, N., Darwish, K. and O’Carroll, C., (1989) Novel regulation of Heat Shock Genes during Carrot Somatic Embryo Development. The Plant Cell 1, 1137–1146.

    Article  PubMed  CAS  Google Scholar 

  30. Györgyey, J., Gartner, A., Németh, K., et al. (1991) Alfalfa heat shock genes are differentially expressed during Somatic Embryogenesis. Plant Mol. Biol. 16, 999–1007.

    Article  PubMed  Google Scholar 

  31. Dong, J. Z. and Dunstan, D. I., (1996) Characterization of three heat-shock-protein genes and their developmental regulation during somatic embryogenesis in white spruce [Picea glauca (Moench) Voss]. Planta 200, 85–91.

    Article  PubMed  CAS  Google Scholar 

  32. Almoguera, C. and Jordano, J. (1992) Developmental and environmental concurrent expression of sunflower dry-seed-stored low-molecular-weigh heat-shock protein and Lea mRNA. Plant Mol. Biol. 19, 781–792.

    Article  PubMed  CAS  Google Scholar 

  33. Coca, M. A., Almoguera, C., Thomas, T. L. and Jordano, J. (1996) Differential regulation of small heat-shock genes in plants: Analysis of a water-stress-inducible and developmentally activated sunflower promoter. Plant Mol. Biol. 31, 863–876.

    Article  PubMed  CAS  Google Scholar 

  34. Zarsky, V., Garrido, D., Eller, N., et al. (1995) The expression of a small heat shock gene is activated during induction of tobacco pollen embryogenesis by starvation 1.- Plant, Cell and Environment 18: 139–147. Plant Cell Environ. 18, 139–147.

    Article  CAS  Google Scholar 

  35. Coca, M. A., Almoguera, C., and Jordano, J. (1994) Expression of sunflower low-molecular-weight heat-shock proteins during embryogenesis and persistence after germination: localization and possible functional implications. Plant Mol. Biol. 25, 479–492.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Apartado Postal 87, Cordemex, Mérida, Yucatán, México

    R. Rojas-Herrera, F. Quiroz-Figueroa, M. Monforte-González, L. Sánchez-Teyer & V. M. Loyola-Vargas

  2. Departamento de Genética y Mejoramiento, Instituto Nacional de Ciencias Agrícolas. Gaveta Postal 1. San José de las Lajas, La Habana, Cuba

    R. Rojas-Herrera

Authors
  1. R. Rojas-Herrera
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Quiroz-Figueroa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Monforte-González
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Sánchez-Teyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. V. M. Loyola-Vargas
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. M. Loyola-Vargas.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rojas-Herrera, R., Quiroz-Figueroa, F., Monforte-González, M. et al. Differential gene expression during somatic embryogenesis in Coffea arabica L., revealed by RT-PCR differential display. Mol Biotechnol 21, 43–50 (2002). https://doi.org/10.1385/MB:21:1:043

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1385/MB:21:1:043

Index Entries

Search

Navigation