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production of transgenic rodents by the microinjection of cloned DNA into fertilized one-cell eggs

Molecular Biotechnology volume 17, pages 151–182 (2001)Cite this article

Abstract

Transgenic technologies that enable rapid movement between genotype and phenotype through specific loss-of-function, overexpression, or misexpression phenotypes will be crucial in the elucidation of gene sequences emerging from genome projects. This article describes detailed procedures for the generation of transgenic mice and rats by the injection of cloned DNA into the pronuclei of fertilized one-cell eggs.

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  1. Molecular Neuroendocrinology Research Group, Univ. Res. Centre for Neuroendocrinology, Univ. Bristol, Bristol Royal Infirmary, Marlborough Street, BS2 8HW, Bristol, UK

    David Murphy

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  1. San Ling Si-Hoe
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  2. Sara Wells
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  3. David Murphy
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Si-Hoe, S.L., Wells, S. & Murphy, D. production of transgenic rodents by the microinjection of cloned DNA into fertilized one-cell eggs. Mol Biotechnol 17, 151–182 (2001). https://doi.org/10.1385/MB:17:2:151

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Index Entries

  • Transgenic rats
  • transgenic mice
  • microinjection
  • superovulation
  • oviduct transfer
  • in vitro fertilization
  • cryopreservation