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Semiquantitative immunoblots of membrane protein-epidermal growth factor

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Abstract

Membrane proteins or cytokines are sometimes difficult to isolate and purify. Our group recently concentrated on epidermal growth factor (EGF) protein expression studies. Mature EGF was initially identified from mouse submaxillary gland extract as a stimulator of eyelid opening and incisor eruption when injected into newborn mice and rats. The EGF precursor is a transmembrane protein with eight additional EGF-like repeats. Our previous study has shown that the EGF precursor without these eight EGF-like repeats (hEGF) was biologically active. Here, we introduce a modified method for rapid detection of hEGF. The membranous protein was directly extracted from various organs of transgenic mice (including the submandibular gland, kidney, liver, heart, and testis) with two different buffers and easily detected by semiquantitative immunoblotting.

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Authors and Affiliations

  1. Department of Paediatrics, the University of Hong Kong, Queen Mary Hospital, Hong Kong

    Siu-Yuen Chan

Authors
  1. Richard Wing-Chuen Wong
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  2. Siu-Yuen Chan
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Correspondence to Siu-Yuen Chan.

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Wong, R.WC., Chan, SY. Semiquantitative immunoblots of membrane protein-epidermal growth factor. Mol Biotechnol 15, 65–67 (2000). https://doi.org/10.1385/MB:15:1:65

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  • DOI: https://doi.org/10.1385/MB:15:1:65

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