Abstract
This article outlines a number of methods for the determination of inhibitory and bactericidal activity against H. pylori. Direct methods rely on the ability of bacteria to divide and multiply and ultimately form visible colonies after subjection to antibiotic treatment. Indirect methods rely on the measurement of metabolic activity as a viability marker and are much more rapid, especially taking into account the slow growth and fastidious nature of the organism. Inhibitory concentration measurement does not indicate the bactericidal ability of a drug; inhibition of growth does not necessarily correlate with cell death. Theoretical generation of viable but nonculturable bacteria could bring in to question the validity of direct measurements based on the colony forming ability of an organism posttreatment.
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References
McNulty, C. A. and Dent, J. C. (1988) Susceptibility of clinical isolates of Campylobacter pylori to twenty one Antimicrobial agents. Eur. J. Clin. Microbiol. Infect. Dis. 7, 566–569.
Lambert, T., Megraud, F., Gerbaud, G., and Courvalin., P. (1986) Susceptibility of Campylobacter pyloridis to 20 antimicrobial agents. Antimicrob. Agents & Chemother. 30, 510–511.
Goodwin, C. S., Blake, P., and Blincow, E. (1986) The minimum inhibitory and bactericidal concentrations of antibiotics and anti-ulcer agents against Campylobacter pyloridis. J. Antimicrob. Chemother. 17, 309–314.
Suerbaum, S., Leying, H., Klemm, K., and Opferkuch, W. (1991) Antibacterial activity of pantoprazole and omeprazole against Helicobacter pylori. Eur. J. Clin. Microbiol. Infect. Dis. 10, 92–93.
Millar, R. M., and Pike, J. (1992) Bactericidal activity of antimicrobial agents against slowly growing Helicobacter pylori. Antimicrob. Agents and Chemother. 36, (N1), 185–187.
Moellering, R. C. (1979) Antimicrobial synergism—An elusive concept. J. Infect. Disease. 140, (N4), 639–641.
McLaren A. and Donnelly., (1997) ATP measurement via bioluminescence to detect bactericidal activity and non-culturable but viable H. pylori. Gut Vol 41, Suppl 1; pA7.
Campbell, A. K. (1988) Chemiluminescence. Principles and applications in Biology and Medicine. Ellis Horwood Ltd, Chichester & VCH Weinheim.
Szalay A. A., Kricka L. J., and Stanley P., E., (1993) Bioluminescence and chemiluminescence: Status Report. Johon Wiley and Sons, Chicester.
Goodwin, C. S., Marshall, B. J., Blincow, E. D., Wilson, D. H., Blackbourn, S., and Philips, M. (1988) Prevention of nitroimidazole resistance in Campylobacter pylori by coadministration of colloidal bismuth subcitrate: clinical and in vitro studies. J. Clin. Pathol. 41, 207–210.
McLaren, A., Donnelly, C., and McDowell, S. R. (1997) The role of Ranitidine bismuth citrate in significantly reducing the emergence of Helicobacter pylori strains resistant to antibiotics. Helicobacter 1997, 1, p21–26.
McLaren, A., McDowell, S. R., Bagshaw, J. A., and McColm, A. A. (1994) The synergistic interaction between GR 122311X and clarithromycin against Helicobacter. Am J. Gastro 898, 1382.
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McLaren, A. Direct and indirect methods of measuring Helicobacter pylori drug susceptibility in vitro. Mol Biotechnol 13, 247–255 (1999). https://doi.org/10.1385/MB:13:3:247
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DOI: https://doi.org/10.1385/MB:13:3:247