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Human identity testing with PCR-based systems

Molecular Biotechnology volume 13, pages 123–136 (1999)Cite this article

Abstract

Large numbers of repetitive stretches of DNA are present within the human genome that are associated with human individuality due to their polymorphic character. Approximately one-third of these repeat sequences is arranged as microsatellites or short tandem repeats (STRs) whose valuable application as state-of-the-art technique in human identity testing will be briefly summarized in this review. Prerequisties for successful DNA typing using STRs amplified by polymerase chain reaction (PCR) are outlined and particular attention is paid to the molecular structure of STRs from autosomes as well as from the Y chromosome. A comprehensive overview about current and emerging methods of STR analysis is given as well.

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Affiliations

  1. Institut für Rechtsmedizin, Universität Münster, Von-Esmarch-Straβe 62, D-48149, Münster, Germany

    Bernd Brinkmann

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  1. Carsten Hohoff
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  2. Bernd Brinkmann
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Correspondence to Bernd Brinkmann.

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Hohoff, C., Brinkmann, B. Human identity testing with PCR-based systems. Mol Biotechnol 13, 123–136 (1999). https://doi.org/10.1385/MB:13:2:123

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Index Entries

  • Short tandem repeats (STRs)
  • PCR
  • allelic ladders
  • forensic validation
  • individual identification
  • DNA typing
  • capillary electrophoresis
  • automated genetic analysis