Abstract
A simple method has been developed that enables reextraction of RNA from an RNA-cDNA mixture. The reextracted RNA was converted to cDNA followed by polymerase chain reaction (PCR). Thus, cDNA synthesis (followed by PCR) was carried out two times on the same source of RNA. The method has been applied to 40 RNA samples of diverse tissue origin with a success rate of 100%. Thus, the method offers more versatile use of small but valuable RNA sources than currently possible.
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References
Franzen, P., ten Dijke, P., Ichijo, H., Yamashita, H., Schulz, P., Heldin, C-H., and Miyazono, K. (1993) Cloning of a TGFβ type I receptor that forms a heteromeric complex with the TGFβ type II receptor. Cell 75, 681–692.
Weber, K., Bolander, M. E., and Sarkar, G. (1998) PIG-B: A homemade monophasic cocktail for the extraction of RNA. Molecular Biotechnology 9, 73–77.
Sarkar, G. and Bolander, M. E. (1994). The “Looped Oligo” method for generating reference molecules for quantitative PCR. Biotechniques, 864–866.
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Fuchs, B., Zhang, K., Rock, M.G. et al. Repeat cDNA synthesis and RT-PCR with the same source of RNA. Mol Biotechnol 12, 231–235 (1999). https://doi.org/10.1385/MB:12:3:231
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DOI: https://doi.org/10.1385/MB:12:3:231