Molecular Biotechnology

, Volume 24, Issue 2, pp 203–220 | Cite as

Multilocus enzyme electrophoresis

A practical guide


Multilocus enzyme electrophoresis (MEE) uses the relative electrophoretic mobilities of intracellular enzymes to characterize and differentiate organisms by generating an electromorph type (ET). This article presents the chemical conditions that may be useful, a guide to the successful practice of the electrophoretic technique, and analysis of the results.

Index Entries

Multilocus enzyme electrophoresis (MEE) epidemiology typing electrophoresis starch gel buffer system Campylobacter 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Lewontin, R. C. and Hubby, J. L. (1966) A molecular approach to the study of the hetrozygosity in natural populations. II. Amount of variation and degree of heterozygosity in natural populations of Drosophila pseudoobscura. Genetics 54, 595–609.PubMedGoogle Scholar
  2. 2.
    Harris, H. and Hopkinson, D. A. (1976) Handbook of Enzyme Electrophoresis in Human Genetics, North-Holland Publishing, Amsterdam.Google Scholar
  3. 3.
    Selander, R. K., Caugant, D. A., Ochman, H., et al. (1986) Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl. Environ. Microbiol. 51, 873–884.PubMedGoogle Scholar
  4. 4.
    Morizot, D. C. and Schmidt, M. E. (1990) Starch gel electrophoresis and histochemical visualisation of proteins, in Electrophoretic and IsoelectricFocusing Techniques in Fisheries Management (Whitmore, D. H., ed.) CRC Press, Boston, pp. 23–80.Google Scholar
  5. 5.
    Rothe, G. (1994) Electrophoresis of Enzymes: Laboratory Methods, Springer-Verlag, Berlin, New York.Google Scholar
  6. 6.
    Sneath, P. H. A. and Sokal, R. R. (1073) Numerical Taxonomy, W. H. Freeman, San Francisco.Google Scholar
  7. 7.
    Graves, L. M. et al. (1994) Comparison of ribotyping and multilocus enzyme electrophoresis for subtyping of Listeria monocytogenes isolates. J. Clin. Microbiol. 32, 2936–2943.PubMedGoogle Scholar
  8. 8.
    Rodriguez, E., De Meeus, T., Mallie, M., et al. (1996) Multicentric epidemiological study of Aspergillus fumigatus isolates by multilocus enzyme electrophoresis J. Clin. Microbiol. 34, 2559–2568.PubMedGoogle Scholar
  9. 9.
    Musser, J. M. and Kapur, V. (1992) Clonal analysis of methicillin-resistant Staphylococcus aureus strains from intercontinental sources: association of the mec gene with divergent phylogenetic lineages implies dissemination by horizontal transfer and recombination. J. Clin. Microbiol. 30, 2058–2063.PubMedGoogle Scholar
  10. 10.
    Pujol, C., Joly S., Lockhard, S. R., et al. (1997) Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and southern blot hybridization with the moderately repetitive DNA probe Ca3 for fingerprinting Candida albicans. J.Clin. Microbiol. 35, 2348–2358.PubMedGoogle Scholar
  11. 11.
    Hall, L. M. C., Whiley, R. A., Duke, B., et al. (1996) Genetic relatedness within and between serotypes of Streptococcus pneumoniae from the United Kingdom: analysis of multilocus enzyme electrophoresis, pulsed-field gel electrophoresis, and antimicrobial resistance patterns. J. Clin. Microbiol. 34, 853–859.PubMedGoogle Scholar
  12. 12.
    Richardson, B. J., Baverstock, P. R., and Adams, M. (1986) Allozyme Electrophoresis, Academic Press, London, New York.Google Scholar
  13. 13.
    Hames, B. D. (1998) Gel Electrophoresis of Proteins, 3rd ed., Oxford University Press, London.Google Scholar

Copyright information

© Humana Press Inc 2003

Authors and Affiliations

  1. 1.Department of BiotechnologyBelfast City HospitalBelfastIreland

Personalised recommendations