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Inherited and experimentally induced changes in gating kinetics of muscle nicotinic acetylcholine receptor

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Abstract

Ligand-gated ion channels (LGIC) allow rapid responses in the nervous system. The nicotonic acetylcholine receptor (AChR) has been the model for structure-function relationship studies on this superfamily. The AChR undergoes the following functional events:

  1. 1.

    Binding of the neurotransmitter.

  2. 2.

    Opening of the ion channel.

  3. 3.

    Conduction of ions across the pore.

  4. 4.

    Desensitization.

The equilibrium among these processes can be perturbed by alteration in the primary structure of the AChR or by the presence of pharmacological agents.

Changes in the primary sequence leading to modifications in gating kinetics may occur in association with physiological or pathological processes. Such changes can also be genetically engineered to gain insights into structure-function relationships.

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Abbreviations

AChR:

nicotinic cholinergic receptor

LGIC:

ligand-gated ion channels

NCI:

noncompetitive inhibitor

PCP:

phencyclidine

HEK:

cells, human embryonic kidney cells

CMS:

congenital myasthenic syndrome

SCCMS:

slow-channel congenital myasthenic syndrome

TID:

3-(trifluoromethyl)3-m-(iodophenyl)diazirine

HC:

hydrocortisone

BSA:

bovine serum albumin

τon :

mean open time

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  1. Instituto Investigaciones Bioquímicas, Universidad Nacional del Sur-CONICET, C.C. 857, 8000, Bahía Blanca, Argentina

    Cecilia Bouzat & Francisco J. Barrantes

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  1. Cecilia Bouzat
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  2. Francisco J. Barrantes
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Bouzat, C., Barrantes, F.J. Inherited and experimentally induced changes in gating kinetics of muscle nicotinic acetylcholine receptor. J Mol Neurosci 13, 1–16 (1999). https://doi.org/10.1385/JMN:13:1-2:1

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