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CD161B: ClrB interactions mediate activation of enhanced lysis of tumor target cells following NK cell:DC co-culture

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Abstract

Co-culture of natural killer (NK) cells and dendritic cells (DCs) results in their reciprocal co-activation, and an enhancement of lysis of tumor target cells. The receptor:ligand pairings mediating this enhancement are unknown. Therefore, we investigated whether interactions of CD161, on NK cells, with Clrs, on DCs, might have a role in this effect. Blocking expression of CD 161B using siRNA resulted in a reduction in enhanced lytic activity following NK:DC co-culture. Conversely, blocking expression of CD161F with siRNA had no effect on enhanced lytic function following NK:DC co-culture. Blocking expression of ClrB/Ocil, a ligand for CD161B, resulted in a reduced level of enhanced lytic function following NK:DC co-culture. This is the first report of NK receptors responsible for interaction with DCs having a role in mediating enhanced lytic function following NK:DC interactions.

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Author notes

  1. William H. Chambers

    Present address: G. 17e Hillman Cancer Center, 5117 Centre Avenue, 15213-1863, Pittsburgh, PA

Authors and Affiliations

  1. G. 17e Hillman Cancer Center, 5117 Centre Avenue, 15213-1863, Pittsburgh, PA

    Tianbing Yang, Melanie S. Flint & Katie M. Webb

  2. Department of Immunology, University of Pittsburgh, 15213, Pittsburgh, PA

    William H. Chambers

  3. Department of Psychology, University of Pittsburgh, 15213, Pittsburgh, PA

    Melanie S. Flint

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  1. Tianbing Yang
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  2. Melanie S. Flint
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  4. William H. Chambers
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Yang, T., Flint, M.S., Webb, K.M. et al. CD161B: ClrB interactions mediate activation of enhanced lysis of tumor target cells following NK cell:DC co-culture. Immunol Res 36, 43–50 (2006). https://doi.org/10.1385/IR:36:1:43

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