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Genome-Wide search for loss of heterozygosity in Chinese patients with sporadic colorectal cancer

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Abstract

In an attempt to integrally investigate the loss of tumor suppressor genes and search for putative suppressor loci associated with tumor occurrence and progression, we conducted a genome-wide loss of heterozygosity (LOH) study of 83 tumor samples obtained from Chinese patients with sporadic colorectal cancer. We employed 400 fluorescence-labeled microsatellite marker primers to amplify the corresponding loci of the genomic DNA and then electrophoresed the polymerase chain reaction products and analyzed the fluorescent signals. The LOH frequencies were high (>35%) but were not associated with the tumor stage and progression in 20 loci, including the regions where TP53, E-cadherin, deleted in colorectal carcinoma (DCC), phosphatase and tensin homolog deleted on chromosome 10 (PTEN), mothers against decapentaplegic, Drosophila, homolog of 2 (MADH2) and mothers against decapentaplegic, Drosophila, homolog of 4 (MADH4) reside. Loss of other loci, including two narrow regions on chromosome 2, was found to relate to the tumor stage, suggesting that this genomic instability may contribute to tumor progression.

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Authors and Affiliations

  1. Shanghai Institute of Physiology, Chinese Academy of Sciences, Shanghai, The People’s Republic of China

    Shaochun Bai, Wanqing Liu & Lin He

  2. Department of Gastrointestinal Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX

    Liwei Wang, Daoyan Wei, Edward Lin & Keping Xie

  3. Department of General Surgery, Shanghai Jiaotong University Affiliated First People’s Hospital, 85 Wujin Road, 200080, Shanghai

    Zhihai Peng, Fang Zhang, Chongzhi Zhou, Yun Ling & Guoqiang Qiu

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  1. Zhihai Peng
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  2. Fang Zhang
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Correspondence to Zhihai Peng.

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Peng, Z., Zhang, F., Zhou, C. et al. Genome-Wide search for loss of heterozygosity in Chinese patients with sporadic colorectal cancer. Int J Gastrointest Canc 34, 39–47 (2003). https://doi.org/10.1385/IJGC:34:1:39

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