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K-ras mutations and benign pancreatic disease

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Summary

This review addresses the history of the ras oncogene, the techniques used to detect molecular alterations in the ras oncogene, and the application of polymerase chain reaction (PCR)-based methods to determine point mutations in clinical samples of patients with pancreatic diseases, namely pancreatic carcinoma and chronic pancreatitis. The frequency of ras mutations in pancreatic carcinoma is high, ranging from 70 to almost 100%. The frequence of ras mutations in chronic pancreatitis, either in pancreatic tissue or pancreatic secretions, vary between 0 and 100%. This wide range in part may be owing to differences in sampling, DNA extraction, or PCR method. The meaning of a k-ras mutation is under debate. Taking into account the positivity of ductal hyperplasias in normal pancreas and ras mutations in normal appearing duct cells, this molecular finding may not mean anything. In contrast, ras mutations are associated with smoking, one acknowledged risk factor for pancreatic carcinoma. The need for large prospective cohort studies is emphasized.

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Authors and Affiliations

  1. Department of Medicine, University of Rostock, Rostock, Germany

    Matthias Löhr

  2. Division of Epidemiology, European Institute of Oncology, Milano, Italy

    Patrick Maisonneuve

  3. Dept. of Surgery, New York Medical College, 10595, Valhalla, NY, USA

    Albert B. Lowenfels (Professor of Surgery)

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  1. Matthias Löhr
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  2. Patrick Maisonneuve
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  3. Albert B. Lowenfels
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Correspondence to Albert B. Lowenfels.

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Löhr, M., Maisonneuve, P. & Lowenfels, A.B. K-ras mutations and benign pancreatic disease. International Journal of Pancreatology 27, 93–103 (2000). https://doi.org/10.1385/IJGC:27:2:093

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  • DOI: https://doi.org/10.1385/IJGC:27:2:093

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