Summary
Methods. Three-month-old female Wistar rats were fed with 20% alcohol in their drinking fluid over 6–17 mo using an interrupted feeding regimen. At different times, pancreatic acini were isolated by mild collagenase digestion. The concentrations of inositol-1,4,5-trisphosphate (1,4,5-IP3) were determined by a specific radioreceptor assay, before and at different times after stimulation with varying concentrations of CCK-8. CCK-induced dynamics of cytoplasmic calcium ([Ca2+]c) was investigated in acinar cells by confocal laser raster microscopy. Acinar α-amylase (Aml) secretion was measured as enzyme activity in the medium compared to the total activity in the suspension.
Results. In 12–13-mo-old rats, the CCK-stimulated 1,4,5-IP3 formation in acini was found to be decreased compared to young rats (age 4 mo). In rats of the same age fed with ethanol from the age of 3 mo on, 1,4,5-IP3 concentrations in acini were higher and reached values comparable to those in young rats. Corre-spondingly, the CCK-induced [Ca2+]c dynamics in acini isolated from 9-mo-old rats was impaired compared to that of young rats but normal in aged, chronically alcohol-fed rats. Aml secretion under CCK stimulation, however, which was decreased in aged rats, was additionally impaired after alcohol feeding.
Conclusion. Chronic alcohol feeding modifies 1,4,5-IP 3 formation, the [Ca2+]c dynamics of, and the Aml secretion of rat pancreatic acini in response to CCK stimulation. Obviously, the age-related impairment of 1,4,5-IP3 formation and [Ca2+]c dynamics is improved. In contrast, the decrease in Aml secretion of acini isolated from aged rats is more pronounced after long-term alcohol-feeding.
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Siegmund, E., Pommerenke, H., Jonas, L. et al. Inositol 1,4,5-trisphosphate formation, cytoplasmic calcium dynamics, and α-amylase secretion of pancreatic acini isolated from aged and chronically alcohol-fed rats. International Journal of Pancreatology 27, 39–50 (2000). https://doi.org/10.1385/IJGC:27:1:39
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DOI: https://doi.org/10.1385/IJGC:27:1:39