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Prolactin-regulated apoptosis of Nb2 lymphoma cells

pim-1, bcl-2, and bax Expression

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Abstract

Lactogen-dependent Nb2 lymphoma cells, widely employed for studying prolactin (PRL) mitogenic mechanisms, are also useful for investigations of apoptosis in T-lineage lymphocytes. Utilizing PRL-dependent Nb2-11 cultures, apoptosis-regulatory genes were evaluated for participation in dexamethasone-(DEX) provoked cell death or its inhibition by PRL. Treatment of lactogen-starved, G1-arrested Nb2-11 cells with DEX (100 nM) activated apoptosis within 12 h evaluated by flow cytometric analysis of fragmented DNA. This effect was not associated with altered expression of bcl-2, bax, or pim-1. PRL (10 ng/mL), coincubated with DEX-treated cells, completely blocked DEX-induced apoptosis. This inhibition was associated with increased expression of bcl-2 and pim-1 mRNAs, genes reported to suppress apoptosis, within 2–6 h after addition of the hormone. Moreover, the increased transcription of bcl-2 and pim-1 was coupled to increases in their protein levels. The results suggest that bcl-2, bax, and pim-1 do not play a critical role in DEX-induced apoptosis in Nb2 cells. However, expression of bcl-2, together with pim-1, may have a role in mediating the antiapoptotic actions of PRL.

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References

  1. Gout P. W., Beer, C. T., and Noble R. L. (1980). Cancer Res. 40, 2433–2436.

    PubMed  CAS  Google Scholar 

  2. Fleming, W. H., Pettigrew, N. M., Matusik, R. J., and Friesen, H. G. (1982). Cancer Res. 42, 3138–3141.

    PubMed  CAS  Google Scholar 

  3. Gout P. W., Horsman, D. E., Fox, K., de Jong, G., Ma, S., and Bruchovsky, N. (1994). Anticancer Res. 14, 2485–2492.

    PubMed  CAS  Google Scholar 

  4. Richards J. F., Beer C. T., Bourgeault C., Chen K., and Gout P. W. (1982). Mol. Cell. Endocrinol. 26, 41–49.

    Article  PubMed  CAS  Google Scholar 

  5. Horseman N. D. (1995). Endocrinology 136, 5249–5251.

    Article  PubMed  CAS  Google Scholar 

  6. Lebron, J. J., Ali, S., Sofer, L., Ullrich, A., and Kelly, P. A. (1994). J. Biol. Chem. 269, 14,021–14,026.

    Google Scholar 

  7. Clevenger, C. V. and Medaglia, M. V. (1994). Mol. Endocrinol. 8, 674–681.

    Article  PubMed  CAS  Google Scholar 

  8. Rao, Y-P., Buckley, D. J., and Buckley, A. R. (1995). Cell Growth Differ. 6, 1235–1244.

    PubMed  CAS  Google Scholar 

  9. Stevens, A. M., Wang, Y-F., Sieger, K. A., Lu, H-F., and Yu-Lee, L-Y. (1995). Mol. Endocrinol. 9, 513–525.

    Article  PubMed  CAS  Google Scholar 

  10. Erwin, R. A., Kirken, R. A., Malabarba, M. G., Farrar, W. L., and Rui, H. (1995). Endocrinology 136, 3512–3518.

    Article  PubMed  CAS  Google Scholar 

  11. Buckley, A. R., Buckley, D. J., Leff, M. A., Hoover, D. S., and Magnuson, N. S. (1995). Endocrinology 136, 5252–5259.

    Article  PubMed  CAS  Google Scholar 

  12. Fletcher-Chiappini, S. E., Compton, M. M., La Voie, H. A., Day, E. B., and Witorsch, R. J. (1993). Proc. Soc. Exp. Biol. Med. 202, 345–352.

    PubMed  CAS  Google Scholar 

  13. Witorsch, R. J., Day, E. B., LaVoie, H. A., Hashemi, N., and Taylor, J. K. (1993). Proc. Soc. Exp. Biol. Med. 203, 454–460.

    PubMed  CAS  Google Scholar 

  14. LaVoie, H. A. and Witorsch, R. J. (1995). Proc. Soc. Exp. Biol. Med. 209, 257–269.

    PubMed  CAS  Google Scholar 

  15. Kerr, J. F., Wyllie, A. H., and Currie, A. R. (1972). Br. J. Cancer 26, 239–257.

    PubMed  CAS  Google Scholar 

  16. Raff, M. C. (1992). Nature 356, 397–400.

    Article  PubMed  CAS  Google Scholar 

  17. Peitsch, M. C., Muller, C., and Tschopp, J. (1993). Nucleic Acids Res. 21, 4206–4209.

    Article  PubMed  CAS  Google Scholar 

  18. Enari, M., Sakahira, H., Yokoyama, H., Okawa, K., Iwamatsu, A., and Nagata, S. (1998) Nature 391, 43–50.

    Article  PubMed  CAS  Google Scholar 

  19. Bakhshi, A., Jensen, J. P., Goldman, P., Wright, J. J., McBride, O. W., Epstein, A. L., et al. (1985). Cell 41, 899–906.

    Article  PubMed  CAS  Google Scholar 

  20. Cleary, M. L. and Sklar, J. (1985). Proc. Natl. Acad. Sci. USA 82, 7439–7443.

    Article  PubMed  CAS  Google Scholar 

  21. Tsujimoto, Y. and Croce, C. M. (1986). Proc. Natl. Acad. Sci. USA 83, 5214–5218.

    Article  PubMed  CAS  Google Scholar 

  22. Oltvai, Z. N., Milliman, C. L., and Korsmeyer, S. J. (1993). Cell 74, 609–619.

    Article  PubMed  CAS  Google Scholar 

  23. Ohta, K., Iwai, K., Kasahara, Y., Taniguchi, N., Krajewski, S., Reed, J. C., et al. (1995). Int. Immunol. 7, 1817–1825.

    Article  PubMed  CAS  Google Scholar 

  24. McDonnell, T. J., Deane, N., Platt, F. M., Nunez, G., Jaeger, U., McKearn, J. P., et al. (1989). Cell 57, 79–88.

    Article  PubMed  CAS  Google Scholar 

  25. McDonnell, T. J., Nunez, G., Platt, F. M., Hockenberry, D., London, L., McKearn, J. P., et al. (1990). Mol. Cell Biol. 10, 1901–1907.

    PubMed  CAS  Google Scholar 

  26. McDonnell, T. J. and Korsmeyer, S. J. (1991). Nature 349, 254–256.

    Article  PubMed  CAS  Google Scholar 

  27. Leff, M. A., Buckley, D. J., Krumenacker, J. S., Reed, J. C., Miyashita, T., and Buckley, A. R. (1996). Endocrinology 137, 5456–5462.

    Article  PubMed  CAS  Google Scholar 

  28. Yin, X. M., Oltval, Z. N., and Korsmeyer, S. J. (1994). Nature 369, 321–323.

    Article  PubMed  CAS  Google Scholar 

  29. Zha, H., Aime-Sempe, C., Sato, T., and Reed, J. C. (1996). J. Biol. Chem. 271, 7440–7444.

    Article  PubMed  CAS  Google Scholar 

  30. Amson, R., Sigaux, F., Przedborski, S., Flandrin, G., Givol, D., and Telerman, A. (1989). Proc. Natl. Acad. Sci. USA 86, 8857–8861.

    Article  PubMed  CAS  Google Scholar 

  31. Meeker, T. C., Nagarajan, L., ar-Rushdi, A., and Croce, C. M. (1987). J. Cell. Biochem. 35, 105–112.

    Article  PubMed  CAS  Google Scholar 

  32. Dautry, F., Weil, D., Yu J., and Dautry-Varsat, A. (1988). J. Biol. Chem. 263, 17,615–17,620.

    CAS  Google Scholar 

  33. Liang, H., Hittelman, W., and Nagarajan, L. (1996). Arch. Biochem. Biophys. 330, 259–265.

    Article  PubMed  CAS  Google Scholar 

  34. Moroy, T., Grzeschiczek, A., Petzold, S., and Hartmann, K. U. (1993). Proc. Natl. Acad. Sci. USA 90, 10,734–10,738.

    Article  CAS  Google Scholar 

  35. Takahashi, C., Harada, Y., Ariga, H., and Iguchi-Ariga, S. M. (1995). Biochem. Biophys. Res. Commun. 215, 538–546.

    Article  PubMed  CAS  Google Scholar 

  36. Nicoletti, I., Migliorati, G., Pagliacci, M. C., Grignani, F., and Riccardi, C. (1991). J. Immunol. Methods 139, 271–279.

    Article  PubMed  CAS  Google Scholar 

  37. Feinberg, A. P. and Vogelstein, B. (1983). Anal. Biochem. 132, 6–13.

    Article  PubMed  CAS  Google Scholar 

  38. Church, G. M. and Gilbert, W. (1984). Proc. Natl. Acad. Sci. USA 81, 1991–1995.

    Article  PubMed  CAS  Google Scholar 

  39. Broome, H. E., Dargan, C. M., Krajewski, S., and Reed, J. C. (1995). J. Immunol. 155, 2311–2317.

    PubMed  CAS  Google Scholar 

  40. Buckley, A. R., Buckley, D. J., Gout, P. W., Liang, H-Q., Rao, Y-P., and Blake, M. J. (1993). Mol. Cell. Endocrinol. 98, 17–25.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology and Toxicology, University of North Dakota School of Medicine and Health Sciences, 501 North Columbia Road, Grand Forks, ND

    Joshua S. Krumenacker, Donna J. Buckley, Matthew A. Leff &  Arthur R. Buckley

  2. Department of Anatomy and Cell Biology, University of North Dakota School of Medicine and Health Sciences, 501 North Columbia Road, Grand Forks, ND

    John T. McCormack

  3. Department of Medical Oncology, British Columbia Cancer Agency, V5Z 4E6, Vancouver, BC, Canada

    Gary de Jong

  4. Department of Cancer Endocrinology, British Columbia Cancer Agency, V5Z 4E6, Vancouver, BC, Canada

    Peter W. Gout

  5. The Burnham Institute, La Jolla, CA

    John C. Reed & Toshiyuki Miyashita

  6. Department of Microbiology, Washington State University, Pullman, WA

    Nancy S. Magnuson

Authors
  1. Joshua S. Krumenacker
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  2. Donna J. Buckley
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Krumenacker, J.S., Buckley, D.J., Leff, M.A. et al. Prolactin-regulated apoptosis of Nb2 lymphoma cells. Endocr 9, 163–170 (1998). https://doi.org/10.1385/ENDO:9:2:163

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  • DOI: https://doi.org/10.1385/ENDO:9:2:163

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