Skip to main content
SpringerLink
Log in

Influence of cortisol on insulin- and insulin-like growth factor 1 (IGF-1)-induced steroid production and on IGF-1 receptors in cultured bovine granulosa cells and thecal cells

  • Published:
Endocrine Aims and scope Submit manuscript

Abstract

During stress, hyperactivity of the adrenal gland can directly and indirectly inhibit ovarian function. However, little evidence existed to support the notion that glucocorticoids could influence insulin-like growth factor 1 (IGF-1) action within the ovary. Therefore, the effect of cortisol on IGF-1-induced granulosa and thecal cell function was evaluated. Granulosa and thecal cells from bovine ovarian follicles were cultured for 2 d in the presence of 10% fetal calf serum and then cultured for an additional 2 d in serum-free medium with added hormones. Cortisol had little or no effect (p>0.05) on IGF-1-induced progesterone production by granulosa cells from both small (1–5 mm) or large (≥8 mm) follicles. Also, cortisol had little or no effect (p>0.05) on basal, insulin-, or IGF-1-induced estradiol production by granulosa cells from small or large follicles, or on the number of IGF-1 receptors in granulosa cells from small follicles. Cortisol had no effect (p>0.10) on insulin-induced granulosa cell numbers, but increased IGF-1-induced granulosa cell numbers. In thecal cells, doses of 1–100 ng/mL of cortisol increased (p<0.05) insulin- and IGF-1-induced thecal cell numbers by 10–20%, progesterone production by 18–36%, and androstenedione production by two- to fourfold. The estimated dose of cortisol necessary to stimulate 50% of the maximum androstenedione production in the presence of IGF-1 was 7 ng/mL. In contrast, cortisol decreased (p<0.05) the number of IGF-1 receptors in thecal cells by 45%. In conclusion, cortisol at physiological levels can directly influence ovarian follicular function in cattle, especially thecal androstenedione production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kanchev, L. N., Dobson, H., Ward, W. R., and Fitzpatrick, R. J. (1976). J. Reprod. Fertil. 48, 341–345.

    Article  PubMed  CAS  Google Scholar 

  2. Stoebel, D. P. and Moberg, G. P. (1982). J. Dairy Sci. 65, 1016–1024.

    PubMed  CAS  Google Scholar 

  3. Smith, E. R., Johnson, J., Weick, R. F., Levine, S., and Davidson, J. M. (1971). Neuroendocrinology 8, 94–106.

    PubMed  CAS  Google Scholar 

  4. Ramaley, J. A. (1976). Proc. Soc. Exp. Biol. Med. 153, 514–517.

    PubMed  CAS  Google Scholar 

  5. Liptrap, R. M. and McNally, P. J. (1976). Am. J. Vet. Res. 37, 369–375.

    PubMed  CAS  Google Scholar 

  6. Refsal, K. R., Jarrin-Maldonado, J. H., and Nachreiner, R. F. (1987). Theriogenology 28, 871–889.

    Article  CAS  Google Scholar 

  7. Kawate, N., Inaba, T., and Mori, J. (1996). J. Vet. Med. Sci. 58, 141–144.

    PubMed  CAS  Google Scholar 

  8. Li, P. S. and Wagner, W. C. (1983). Biol. Reprod. 29, 25–37.

    Article  PubMed  CAS  Google Scholar 

  9. Padmanabhan, V., Keech, C., and Convey, E. M. (1983). Endocrinology 112, 1782–1787.

    PubMed  CAS  Google Scholar 

  10. Suter, D. E. and Schwartz, N. B. (1985). Endocrinology 117, 849–854.

    PubMed  CAS  Google Scholar 

  11. Baldwin, D. M., Srivastava, P. S., and Krummen, L. A. (1991). Biol. Reprod. 44, 1040–1050.

    Article  PubMed  CAS  Google Scholar 

  12. Hsueh, A. J. W. and Erickson, G. F. (1978). Steroids 32, 639–648.

    Article  PubMed  CAS  Google Scholar 

  13. Schoonmaker, J. N. and Erickson, G. F. (1983). Endocrinology 113, 1356–1363.

    Article  PubMed  CAS  Google Scholar 

  14. Ginther, O. J., Wiltbank, M. C., Fricke, P. M., Gibbons, J. R., and Kot, K. (1996). Biol. Reprod. 55, 1187–1194.

    Article  PubMed  CAS  Google Scholar 

  15. Spicer, L. J. and Echternkamp, S. E. (1986). J. Anim. Sci. 62, 428–451.

    PubMed  CAS  Google Scholar 

  16. Schreiber, J. R., Nakamura, K., and Erickson, G. F. (1982). Steroids 39, 569–584.

    Article  PubMed  CAS  Google Scholar 

  17. Spicer, L. J. and Echternkamp, S. E. (1995). Domestic Anim. Endocrinology 12, 223–245.

    Article  CAS  Google Scholar 

  18. Kaplowitz, P. B. (1987). J. Clin. Endocrinology Metab. 64, 563–571.

    CAS  Google Scholar 

  19. Bird, J. L. E. and Tyler, J. A. (1994). J. Endocrinology 142, 571–579.

    CAS  Google Scholar 

  20. Giorgino, F. and Smith, R. J. (1995). J. Clin. Invest. 96, 1473–1483.

    Article  PubMed  CAS  Google Scholar 

  21. Stewart, R. E., Spicer, L. J., Hamilton, T. D., and Keefer, B. E. (1995). J. Anim. Sci. 73, 3719–3731.

    PubMed  CAS  Google Scholar 

  22. Spicer, L. J., Alpizar, E., and Vernon, R. K. (1994). Mol. Cell. Endocrinology 102, 69–76.

    Article  CAS  Google Scholar 

  23. Scatchard, G. (1949). Ann. NY Acad. Sci. 51, 600–672.

    Article  Google Scholar 

  24. da Rosa, G. O. and Wagner, W. C. (1981). J. Anim. Sci. 52, 1098–1105.

    PubMed  Google Scholar 

  25. Echternkamp, S. E. (1984). Theriogenology 22, 305–311.

    Article  PubMed  CAS  Google Scholar 

  26. Dobson, H., Alam, M. G. S., and Kanchev, L. N. (1987). J. Reprod. Fertil. 80, 25–30.

    Article  PubMed  CAS  Google Scholar 

  27. Hein, K. G. and Allrich, R. D. (1992). J. Anim. Sci. 70, 243–247.

    PubMed  CAS  Google Scholar 

  28. Garverick, H. A., Erb, R. E., Niswender, G. D., and Callahan, C. J. (1971). J. Anim. Sci. 32, 946–956.

    PubMed  CAS  Google Scholar 

  29. Swanson, L. V., Hafs, H. D., and Morrow, D. A. (1972). J. Anim. Sci. 34, 284–293.

    PubMed  CAS  Google Scholar 

  30. Roussel, J. D., Clement, T. J., Aranas, T. J., and Seybt, S. H. (1983). Theriogenology 19, 535–539.

    Article  PubMed  CAS  Google Scholar 

  31. Zinn, S. A., Purchas, R. W., Chapin, L. T., Petitclerc, D., Merkel, R. A., Bergen, W. G., et al. (1986). J. Anim. Sci. 63, 1804–1815.

    PubMed  CAS  Google Scholar 

  32. Spicer, L. J. and Zinn, S. A. (1987). J. Reprod. Fertil. 81, 221–226.

    Article  PubMed  CAS  Google Scholar 

  33. Boandl, K. E., Wohlt, J. E., and Carsia, R. V. (1989). J. Dairy Sci. 72, 2193–2197.

    PubMed  CAS  Google Scholar 

  34. Dehennin, L., Nahoul, L. K., and Scholler, R. (1987). J. Steroid Biochem. 26, 337–343.

    Article  PubMed  CAS  Google Scholar 

  35. Fateh, M., Ben-Rafael, Z., Benadiva, C. A., Mastroianni, L., Jr., and Flickinger, G. L. (1989). Fertil. Steril. 51, 538–541.

    PubMed  CAS  Google Scholar 

  36. Jimena, P., Castilla, J. A., Peran, F., Ramirez, J. P., Vergara, F., Jr., Molina, R., et al. (1992). Acta Endocrinol. 127, 403–406.

    PubMed  CAS  Google Scholar 

  37. Spicer, L. J., Convey, E. M., Leung, K., Short, R. E., and Tucker, H. A. (1986). J. Anim. Sci. 62, 742–750.

    PubMed  CAS  Google Scholar 

  38. Kawate, N., Inaba, T., and Mori, J. (1993). Anim. Reprod. Sci. 32, 15–25.

    Article  CAS  Google Scholar 

  39. Ball, E. H. and Sanwal, B. D. (1980). J. Cell Physiol. 102, 27–36.

    Article  PubMed  CAS  Google Scholar 

  40. Guerriero, V., Jr. and Florini, J. R. (1980). Endocrinology 106, 1198–1202.

    PubMed  CAS  Google Scholar 

  41. Amin, W., Karlan, B. Y., and Littlefield, B. A. (1987). Cancer Res. 47, 6040–6045.

    PubMed  CAS  Google Scholar 

  42. Kream, B. E., Petersen, D. N., and Raisz, L. G. (1990). Endocrinology 126, 1576–1583.

    PubMed  CAS  Google Scholar 

  43. Stewart, A. G., Fernandes, D., and Tomlinson, P. R. (1995). Br. J. Pharmacol. 116, 3219–3226.

    PubMed  CAS  Google Scholar 

  44. de Greef, W. J. and van der Schoot, P. (1987). Acta Endocrinol. 116, 350–356.

    PubMed  Google Scholar 

  45. Daly, D. C., Walters, C. A., Soto-Albors, C. E., Tohan, N., and Riddick, D. H. (1984). Fertil. Steril. 41, 844–848.

    PubMed  CAS  Google Scholar 

  46. Danisova, A., Sebokova, E., and Kolena, J. (1987). Endocrinol. Experimentia 21, 13–22.

    CAS  Google Scholar 

  47. Adashi, E. Y., Jones, P. B. C., and Hsueh, A. J. W. (1981). Endocrinology 109, 1888–1894.

    Article  PubMed  CAS  Google Scholar 

  48. Luck, M. R. (1988). J. Reprod. Fertil. 83, 901–907.

    Article  PubMed  CAS  Google Scholar 

  49. Ben-Rafael, Z., Benadiva, C. A., Garcia, C. J., and Flickinger, G. L. (1988). Fertil. Steril. 49, 813–816.

    PubMed  CAS  Google Scholar 

  50. Fitzpatrick, S. L. and Richards, J. S. (1991). Endocrinology 129, 1452–1462.

    PubMed  CAS  Google Scholar 

  51. Spicer, L. J. and Stewart, R. E. (1996). J. Dairy Sci. 79, 813–821.

    Article  PubMed  CAS  Google Scholar 

  52. Spicer, L. J., Alpizar, E., and Echternkamp, S. E. (1993). J. Anim. Sci. 71, 1232–1241.

    PubMed  CAS  Google Scholar 

  53. Spicer, L. J., Alpizar, E., and Stewart, R. E. (1994). Endocrine 2, 735–739.

    CAS  Google Scholar 

  54. Danisova, A., Sebokova, E., and Kolena, J. (1987). Exp. Clin. Endocrinology 89, 165–173.

    CAS  Google Scholar 

  55. Botero, L. F., Roberts, C. T., Jr., LeRoith, D., Adashi, E. Y., and Hernandez, E. R. (1993). Endocrinology 132, 2703–2708.

    Article  PubMed  CAS  Google Scholar 

  56. Papa, V., Hartmann, K. K., Rosenthal, S. M., Madux, B. A., Siiteri, P. K., and Goldfine, I. D. (1991). Mol. Endocrinology 5, 709–717.

    Article  CAS  Google Scholar 

  57. Langhout, D. J., Spicer, L. J., and Geisert, R. D. (1991). J. Anim. Sci. 69, 3321–3334.

    PubMed  CAS  Google Scholar 

  58. Roberts, A. J. and Skinner, M. K. (1990). Mol. Cell. Endocrinology 72, R1-R5.

    Article  CAS  Google Scholar 

  59. SAS/STAT (1988). User’s Guide. SAS Institute, Inc., Cary, NC.

    Google Scholar 

  60. Ott, L. (1977). In: An Introduction to Statistical Methods and Data Analysis. Duxbury Press, North Scituate, MA, p. 384.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Animal Science, Oklahoma State University, 74078, Stillwater, OK

    Leon J. Spicer & Connie S. Chamberlain

Authors
  1. Leon J. Spicer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Connie S. Chamberlain
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Leon J. Spicer.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Spicer, L.J., Chamberlain, C.S. Influence of cortisol on insulin- and insulin-like growth factor 1 (IGF-1)-induced steroid production and on IGF-1 receptors in cultured bovine granulosa cells and thecal cells. Endocr 9, 153–161 (1998). https://doi.org/10.1385/ENDO:9:2:153

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/ENDO:9:2:153

Key Words

Search

Navigation