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Induction of an intronic enhancer of the human ciliary neurotrophic factor receptor (CNTFRα) gene by the TR3 orphan receptor

Endocrine volume 9, pages 27–32 (1998)Cite this article

Abstract

A hormone response element, CNTFRα-NBRE (5′-AAAGGTCA-3′) has been identified in the fifth intron of the α component of ciliary neurotrophic factor receptor gene (CNTFR-I5) for the human TR3 orphan receptor (TR3). A specific binding between in vitro expressed TR3 and CNTFRα-NBRE was demonstrated by using electrophoretic mobility shift assay. A reporter gene assay using chloramphenicol acetyltransferase (CAT) showed that CNTFR-I5 has an enhancer activity that could be induced by TR3 in a dose-dependent manner. This induction was significantly reduced in the absence of CNTFRα-NBRE. Together, these results indicate CNTFRα-NBRE is sufficient to mediate TR3 action in inducing the enhancer activity of CNTFR-I5. Our finding may, therefore, suggest CNTFRα is a target gene regulated by TR3 and expand the role of TR3 in the nervous system.

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Abbreviations

CNTFRα:

alpha component of the ciliary neurotrophic factor

CNTFR-I5:

the fifth intron of the CNTFRα gene

TR3:

TR3 orphan receptor

NBRE:

TR3/NGFI-B/nur77 response element

DR1:

direct repeat with 1-bp spacing

SDS:

sodium dodecyl sulfate

TNT:

coupled in vitro transcription and translation

CHO:

Chinese hamster ovary cell

CAT:

chloramphenicol acetyltransferase

EMSA:

electrophoretic mobility shift assay

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Affiliations

  1. George Whipple Lab for Cancer Research, Departments of Pathology, Urology, and Radiation Oncology, University of Rochester, Rochester, NY

    Xiao-min Mu, Win-Jing Young, Hiroji Uemura &  Chawnshang Chang

  2. Institute of Zoology, Chinese Academy of Sciences, China

    Yi-xun Liu

Authors
  1. Xiao-min Mu
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  2. Win-Jing Young
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  3. Yi-xun Liu
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  4. Hiroji Uemura
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  5. Chawnshang Chang
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Correspondence to Chawnshang Chang.

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Mu, Xm., Young, WJ., Liu, Yx. et al. Induction of an intronic enhancer of the human ciliary neurotrophic factor receptor (CNTFRα) gene by the TR3 orphan receptor. Endocr 9, 27–32 (1998). https://doi.org/10.1385/ENDO:9:1:27

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  • DOI: https://doi.org/10.1385/ENDO:9:1:27

Key Words

  • TR3
  • CNTFRα
  • NBRE
  • intronic enhancer