Abstract
Streptozotocin-(STZ) treated diabetic male rats have significant reproductive endocrinopathy. To determine the functional responsiveness of luteinizing hormone-releasing hormone (LHRH) neurons in STZ-treated diabetic male rats, stimulated LHRH release was assessed using hypothalami from short-term STZ-treated, STZ-treated insulin-replaced, and control male rats. LHRH release from control, STZ-treated, and STZ-treated, insulin-replaced explants in response to an initial and second 30-min pulse of phenylephrine were not different. A terminal pulse, containing 45 mM KCI, a general secretogogue, also revealed no differences between groups in stimulated LHRH release. Glucose and testosterone levels in the controls and the diabetic rats were significantly different. Cell counts on serial brain sections processed for LHRH immunohistochemistry suggested that the number of LHRH neurons in the preoptic arear (POA) and septal areas were not different between control and STZ-treated rats. Thus, the short-term STZ-treated rats of this study were diabetic, and they displayed associated endocrinopathy; however, explants obtained from control and STZ-treated rats exhibited a typical LHRH responsiveness to both phenylephrine and KCI, and appeared similar in LHRH neuron number. Therefore, these findings suggest that reproductive endocrinopathy accompanying short-term STZ-induced diabetes in male rats does not result from deficiency in LHRH neurons per se.
Similar content being viewed by others
References
Cameron, D. F., Murray, F. T. and Drylie, D. M. (1985). Anat. Rec. 213, 53–62.
Faerman, I., Vilar, O., Rivarola, M. A., Rosner, J. M., Jadzinsky, M. N. and Fox, D. (1972). Diabetes 21, 23–30.
Kolodny, R. C., Kahn, C. B., Goldstein, H. H. and Barnett, D. M. (1974). Diabetes 23, 306–309.
Murray, F. T., Wyss, H. U., Thomas, R. G., Spevack, M. and Glaros, A. G. (1987). J. Clin. Endocrinol. Metab. 65, 127–135.
Yamauchi, S. (1965). Jpn. J. Urol. 56, 715–720.
Fairburn, C. G. (1981). Br. J. Hosp. Med. 25, 484–491.
Ficher, M., Zukerman, M., Fishkin, R. E., Goldman, A., Neeb, M., and Fink, P. J. (1984). J. Androl. 5, 8–16.
Steger, R. W., Amador, A., Lam, E., Rathert, J., Weis, J. and Smith, M. S. (1989). Endocrinology 124, 1737–1743.
Calvo, J. C., Baranao, J. L., Tesone, M. and Charreau, E. H. (1984). J. Steroid Biochem. 20, 769–772.
Howland, B. E. and Zebrowski, E. J. (1976). Horm. Metab. Res. 8, 465–472.
Jackson, F. L. and Hutson, J. C. (1984). Diabetes 33, 819–824.
Steger, R. W. (1990). Pharmacol. Biochem. Behav. 35, 577–582.
Howland, B. E. and Zebrowski, E. J. (1980). Experientia 36, 610–613.
King, T. S. and Kang, I. S. (1989). J. Biomed. Res. 10(5), 1–8.
Bestetti, G. E., Boujon, C. E., Reymond, M. J. and Rossi, G. L. (1989). Diabetes 38, 471–476.
Chandrashekar, V., Steger, R. W., Bartke, A., Fadden, C. T. and Kienast S. G. (1991). Neuroendocrinol. 54, 30–45.
Barraclough, C. A. and Wise, P. M. (1982). Endocr. Rev. 3, 91–119.
Clough, R. W., Hoffman, G. E. and Sladek, C. D. (1988). Brain Res. 446, 121–132.
Clough, R. W., Hoffman, G. E. and Sladek, C. D. (1990). Neuroendocrinol. 51, 131–138.
Kalra, S. P. and Kalra, P. S. (1983). Endocr. Rev. 4, 311–351.
Negro-Vilar, A., Ojeda, S. R. and McCann, S. M. (1979). Endocrinology 104, 1749–1757.
Steger, R. W. and Kienast, S. G. (1990). Diabetes 39(8), 942–948.
Simpkins, J. W., Kalra, P. S. and Kalra, S. P. (1980). Neuroendocrinology 30, 94–100.
Leadem, C. A., Crowley, J. W., Simpkins, J. W. and Kalra, S. P. (1985). Neuroendocrinology 29, 288–295.
Wilkes, M. M. and Yen, S. S. C. (1981). Life Sci. 28, 2355–2359.
Lamberts, R., Vijayan, E., Graf, M., Mansky, T. and Wuttke, W. (1983). Exp. Brain Res. 52, 356–362.
McKelvy, J. F., LeBlanc, P., Laudes, C., Perrie, S., Grimm-Jorgensen, Y. and Kordon, C. (1976). Biochem. Biophys. Res. Commun. 73, 507–575.
Hartter, D. E. and Ramirez, V. D. (1985). Neuroendocrinol. 40, 476–482.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Clough, R.W., Kienast, S.G. & Steger, R.W. Reproductive endocrinopathy in acute streptozotocin-induced diabetic male rats. Endocr 8, 37–43 (1998). https://doi.org/10.1385/ENDO:8:1:37
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/ENDO:8:1:37