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CRH-stimulation of cyclic adenosine 5′-monophosphate pathway is partially inhibited by the coexpression of CRH-R1 and CRH-R2α

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An Erratum to this article was published on 01 August 2005

Abstract

Corticotropin-releasing hormone (CRH) is one of the major proteins responsible for brain stress regulation. Two well-known receptors have been described: type 1 and type 2α, both members of the receptor superfamily of G protein-coupled receptors (GPCR). We investigated receptor regulation when both CRH receptor subtypes are coexpressed in the same mammalian cell line. When both types of receptors are coexpressed, cAMP second messenger production is partially inhibited compared to when receptors are expressed separately. However, neither binding kinetics nor internalization rates are modified by coexpression of these receptors. To our knowledge this is the first demonstration of receptor interaction that results in the modification of CRH-mediated signal transduction pathway. Because CRH-R1 and CRH-R2α have overlapping mRNA expression patterns in the brain, these receptors may be coexpressed in neurons, suggesting that receptor interaction may play an important role in the effect evoked by CRH, contributing to the complexity of differential coupling of the CRH receptors in different endocrine and stress behavior responses.

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Authors and Affiliations

  1. Research Unit in Developmental Biology, Hospital de Pediatría, Centro Médico Nacional Siglo SXXI, Instituto Mexicano del Seguro Social, Mexico

    G. Maya-Núñez, C. Castro-Fernández & P. Méndez

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  1. G. Maya-Núñez
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  2. C. Castro-Fernández
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  3. P. Méndez
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Correspondence to P. Méndez.

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An erratum to this article is available at http://dx.doi.org/10.1007/s12020-005-0001-0.

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Maya-Núñez, G., Castro-Fernández, C. & Méndez, P. CRH-stimulation of cyclic adenosine 5′-monophosphate pathway is partially inhibited by the coexpression of CRH-R1 and CRH-R2α. Endocr 27, 67–73 (2005). https://doi.org/10.1385/ENDO:27:1:067

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  • DOI: https://doi.org/10.1385/ENDO:27:1:067

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