Abstract
We recently isolated 3-methyl-4-nitrophenol (4-nitro-m-cresol; PNMC) and 4-nitro-3-phenylphenol (PNMPP) from diesel exhaust particles (DEP) and identified them as vasodilators and xenoestrogens. The estrogenic activity of PNMC and PNMPP was further examined by using immunohistochemical staining of proliferating cell nuclear antigen (PCNA) in uterine luminal epithelium of ovariectomized 25-d-old immature female rats injected with PNMC and PNMPP subcutaneously for 2 d. Significant increases were observed in uterine luminal epithelium in PCNA positive cells of animals receiving 10 and 100 mg/kg PNMC and 0.1 mg/kg PNMPP compared with controls. These results clearly show the estrogenic activity of PNMC and PNMPP by cell proliferation on the uterine luminal epithelium.
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References
McClellan, R. O. (1987). Annu. Rev. Pharmacol. Toxicol. 27, 279–300.
Ichinose, T., Yajima, Y., Nagashima, M., Takenoshita, S., Nagamachi, Y., and Sagai, M. (1997). Carcinogenesis 18, 185–192.
Muranaka, M., Suzuki, S., Koizumi, K., et al. (1986). J. Allergy Clin. Immunol. 77, 616–623.
Takafuji, S., Suzuki, S., Koizumi, K., et al. (1987). J. Allergy Clin. Immunol. 79, 639–645.
Sagai, M., Saito, H., Ichinose, T., Kodama, M., and Mori, Y. (1993). Free Radic. Biol. Med. 14, 37–47.
Miyabara, Y., Ichinose, T., Takano, H., and Sagai, M. (1998). Int. Arch. Allergy Immunol. 116, 124–131.
Yoshida, S., Sagai, M., Oshio, S., et al. (1999). Int. J. Androl. 22, 307–315.
Tsukue, N., Toda, N., Tsubone, H., et al. (2001). J. Toxicol. Environ. Health. A 63, 115–126.
Tsukue, N., Toda, N., Sagai, M., et al. (2002). Envirn. Sci. 9, 355–367.
Tsukue, N., Tsubone, H., and Suzuki, A. K. (2002). Inhal. Toxicol. 14, 635–651.
Taneda, S., Hayashi, H., Sakushima, A., et al. (2002). Toxicology 170, 153–161.
Taneda, S., Hayashi, H., Sakushima, A., et al. (2002). Environ. Sci. 9, 301–308.
Mori, Y., Taneda, S., Hayashi, H., et al. (2002). Biol. Pharm. Bull. 25, 145–146.
Mori, Y., Kamata, K., Toda, N., et al. (2003). Biol. Pharm. Bull. 26, 394–395.
Taneda, S., Kamata, K., Hayashi, H., et al. (2003). J. Health Sci. 50, 133–141.
Furuta, C., Suzuki, A. K., Taneda, S., et al. (2004). Biol. Reprod. 70, 1527–1533.
Wata, T., Shimabukuro, T., Kurisu, H., et al. (1993). Hinyokika Kiyo 39, 313–319.
Casasco, A., Giordano, M., Danova, M., et al. (1993). Histochemistry 99, 191–199.
Retha, R. N., Wendy, N. J., Elizabeth, P. B., and Joseph, H. (2004). Reprod. Toxicol. 18, 399–406.
Morita, T., Usuda, N., Hanai, T., and Nagata, T. (1994). Histochemistry 101, 13–20.
Ekena, K., Katzenellenbogen, J. A., and Katzenellenbogen, B. S. (1998). J. Biol. Chem. 273, 693–699.
Ekena, K., Weis, K. E., Katzenellenbogen, J. A., and Katzenellenbogen, B. S. (1996). J. Biol. Chem. 271, 20053–20059.
Ekena, K., Weis, K. E., Katzenellenbogen, J. A., and Katzenellenbogen, B. S. (1997). J. Biol. Chem. 272, 5069–5075.
Steinmetz, R., Brown, N. G., Allen, D. L., Bigsby, R. M., and Ben-Jonathan, N. (1997). Endocrinology 138, 1780–1786.
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Furuta, C., Li, C., Taneda, S. et al. Immunohistological study for estrogenic activities of nitrophenols in diesel exhaust particles. Endocr 27, 33–36 (2005). https://doi.org/10.1385/ENDO:27:1:033
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DOI: https://doi.org/10.1385/ENDO:27:1:033