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Plasma concentrations of ir-inhibin, inhibin A, inhibin pro-αC, FSH, and estradiol-17β during estrous cycle in mares and their relationship with follicular growth

Endocrine volume 25, pages 7–14 (2004)Cite this article

Abstract

The relationship among plasma levels of immunoreactive (ir)-inhibin, inhibin A, inhibin pro-αC, FSH, estradiol-17β and follicular growth were investigated during the normal estrous cycle in mares. Seven mares were used for two successive normal estrous cycles. Follicular diameters and ovulation were obtained by transrectal ultrasonography, and blood samples were collected daily by jugular venipuncture for hormonal assay. The results showed that inhibin A was inversely correlated (r=−0.59, p<0.0001) with FSH indicating its contribution to negative feedback control of FSH secretions from the pituitary gland. Estradiol-17β increased during the follicular phase reaching a peak (37.9±3.8 pg/mL) 2 d before ovulation. Estradiol-17β was positively correlated (r=0.78, p<0.0001) with inhibin A. The high levels of inhibin A and estradiol-17β were associated with the growth of the preovulatory dominant follicle and inversely correlated with FSH suggesting that both hormones are products of the large dominant follicles and were responsible for the decline in FSH secretion during the follicular phase of estrous cycle. In conclusion, an inverse relationship between inhibin A and FSH was clearly demonstrated indicating that inhibin A has a key role in the negative feedback control of FSH from the pituitary gland. In addition, inhibin A and estradiol-17β secretions were associated with the growth of the preovulatory dominant follicle and were positively correlated.

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Affiliations

  1. Labortory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, 183-8509, Tokyo, Japan

    Mohamed S. Medan, Gen Watanabe & Kazuyoshi Taya DVM PhD

  2. Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt

    Mohamed S. Medan

  3. Hidaka Training Research Center, Japan Racing Association, 057-171, Hokkaido, Japan

    Yasuo Nambo

  4. Northern Farm, 059-4132, Hokkaido, Japan

    Natsuko Nagamine

  5. Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan

    Hiromi Shinbo, Gen Watanabe & Kazuyoshi Taya DVM PhD

  6. School of Biological and Molecular Sciences, Oxford Brookes University, OX3 OBP, Oxford, UK

    Nigel Groome

Authors
  1. Mohamed S. Medan
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  2. Yasuo Nambo
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  3. Natsuko Nagamine
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  4. Hiromi Shinbo
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  5. Gen Watanabe
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  6. Nigel Groome
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  7. Kazuyoshi Taya DVM PhD
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Correspondence to Kazuyoshi Taya DVM PhD.

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Medan, M.S., Nambo, Y., Nagamine, N. et al. Plasma concentrations of ir-inhibin, inhibin A, inhibin pro-αC, FSH, and estradiol-17β during estrous cycle in mares and their relationship with follicular growth. Endocr 25, 7–14 (2004). https://doi.org/10.1385/ENDO:25:1:07

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  • DOI: https://doi.org/10.1385/ENDO:25:1:07

Key Words

  • Inhibin
  • FSH
  • estradiol
  • follicle
  • mare