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Heparin increases prolactin and modifies the effects of FGF-2 upon prolactin accumulation in pituitary primary cultures

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Abstract

We have studied the effects of heparin on prolactin accumulation in the medium from primary pituitary cultures, and whether heparin interferes with the effects of fibroblast growth factor-2 (FGF-2) on PRL regulation in vitro. In the absence of exogenous FGF-2, heparin increased prolactin accumulation in the culture medium in a dose-dependent manner. FGF-2 also increased the prolactin levels of primary cells in a time-and dose-dependent manner. However, low doses of heparin reduced the effects of FGF-2, but higher doses of heparin increased the maximal FGF-2-induced prolactin secretion and ED50. In vivo estrogenization of rats resulted in the abolition of FGF-2 capability to promote prolactin release in vitro. However, heparin restored cell responsiveness to FGF-2.

Our results suggest that heparin, when present in the medium, binds FGF-2, therefore reducing its ability to interact with FGF receptors in a dose-dependent manner up to a critical molar concentration, at which heparin itself starts to activate the FGF receptor, and strengthens the activation induced by its proper ligand, FGF-2. Prolactin responses to FGF-2 are blocked by estrogen pretreatment, and it is probable that this introduces lactotroph cells in the proliferative stage. In conclusion, heparin modulates PRL secretion and PRL responses to FGF-2 in vitro.

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Authors and Affiliations

  1. Cajal Institute, CSIC, Avd. Dr. Arce, 37, 28.002, Madrid, Spain

    Carlos Spuch

  2. Laboratoire Homéosthasie, Allostasie, Pathologie, Université Victor Ségalen, Bourdeaux 2, Rué Léo Saignat, 146, 33.076, Bourdeaux, France

    Yolanda Diz-Chaves

  3. Department of Functional Biology and Health Sciences, Laboratory of Endocrinology, Faculty of Biology, Campus of Vigo, University of Vigo, 36.200, Vigo, Spain

    Diego Pérez-Tilve & Federico Mallo

Authors
  1. Carlos Spuch
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  2. Yolanda Diz-Chaves
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  3. Diego Pérez-Tilve
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  4. Federico Mallo
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Correspondence to Carlos Spuch.

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Spuch, C., Diz-Chaves, Y., Pérez-Tilve, D. et al. Heparin increases prolactin and modifies the effects of FGF-2 upon prolactin accumulation in pituitary primary cultures. Endocr 24, 131–136 (2004). https://doi.org/10.1385/ENDO:24:2:131

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  • DOI: https://doi.org/10.1385/ENDO:24:2:131

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