Abstract
Activity of the magnocellular neurons that synthesize vasopressin and oxytocin in the paraventricular and supraoptic nuclei of the hypothalamus can be modulated by local release of neuromediators within the nuclei. Among the bioactive peptides that may play autocrine or paracrine roles in this system is prolactin (PRL). Paraventricular and supraoptic neurons express PRL mRNA and contain and secrete PRL-like proteins of 23 and 14 kDa. We investigated the localization of PRL receptors in vasopressinergic and oxytocinergic magnocellular neurons using dual-label immunofluorescence. The results demonstrate that both vasopressin- and oxytocin-immunoreactive cells of the paraventricular and supraoptic nuclei contain the PRL receptor. In addition, we investigated the possible regulation of vasopressin secretion by PRL using hypothalamo-neurohypophyseal explants in culture. The results show that PRL and a 16 kDa N-terminal fragment of the hormone that is analogous to the neurohypophyseal 14-kDa PRL fragment stimulate the release of vasopressin. Together, these findings support the hypothesis that vasopressinergic and oxytocinergic neurons of the magnocellular secretory system are regulated directly by various isoforms of PRL via autocrine/paracrine mechanisms.
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Brownstein, M. J., Russell, J. T., and Gainer, H. (1980). Science 207, 373–378.
Robertson, G. L. (1976). Recent. Prog. Horm. Res. 33, 333–385.
Wakerley, J. B., Clarke, G., and Summerlee, A. J. S. (1988). In: The physiology of reproduction. Knobil, E. and Neill, J. (eds.). Raven: New York.
Moos, F., Freund-Mercier, Y., Guerné, Y., Guerné, J. M., Stoecke, M. E., and Richard, P. (1984). J. Endocrinol. 102, 63–72.
Brownstein, M. J. and Mezey, E. (1986). In: Progress in brain research. Hokfelt, T., Fuxe, K., and Pernow, B. (eds.). Elsevier: Amsterdam.
Sawchenko, P. E., Imaki, T., and Vale, W. (1992). In: Functional anatomy of the neuroendocrine hypothalamus. Ciba Foundation Symposium No. 168. John Wiley: London.
Larsen, P. J., Jessop, D. S., Chowdrey, H. S., Lightman, S. L., and Chowdrey, H. S. (1992). Neurosci. Lett. 138, 23–26.
Sladek, C. D. and Joynt, R. J. (1980). Endocrinology 106, 173–178.
Larsen, P. J., Jukes, K. E., Chowdrey, H. S., Lightman, S. L., and Jessop, D. S. (1994). Endocrinology 134, 1635–1639.
Pow, D. V. and Morris, J. F. (1989). Neuroscience 32, 435–439.
Ludwig, M. and Leng, G. (1997). Eur. J. Neurosci. 9, 2532–2540.
Tobeau, G., Desclin, J., Tier, M., and Pasteels, J. L. (1979). J. Endocrinol. 83, 261–266.
Paut-Pagano, L., Roky, R., Valatx, J.-L., Kitahama, K., and Jouvet, M. (1993). Neuroendocrinology 58, 682–695.
Clapp, C., Torner, L., Gutiérrez-Ospina, G., et al. (1994). Proc. Natl. Acad. Sci. USA 91, 10384–10388.
Torner, L., Nava, G., Dueñas, Z., et al. (1999). J. Endocrinol. 161, 423–432.
López-Gómez, F. J., Torner, L., Mejía, S., Martínez de la Escalera, G., and Clapp, C. (1995). Endocrine 3, 573–578.
Mejía, S., Morales, M. A., Zetina, M. E., Martínez de la Escalera, G., and Clapp, C. (1997). Neuroendocrinology 66, 151–159.
Torner, L., Mejía, S., López-Gómez, F. J., Quintanar, A., Martínez de la Escalera, G., and Clapp, C. (1995). Endocrinology 136, 5454–5460.
Roky, R., Paut-Pagano, L., Goffin, V., et al. (1996). Neuroendocrinology 63, 422–429.
Barowska, J. C. and Morrell, J. I. (1997). J. Comp. Endocrinol. 386, 161–177.
Crumeyrolle-Arias, M., Latouche, J., Jammes, H., et al. (1993). Neuroendocrinology 57, 457–466.
Gosh, R. and Sladek, C. D. (1995). Brain Res. 672, 24–28.
Parker, S. L., Armstrong, W. E., Sladek, C. D., Grosvenor, C. E., and Crowley, W. R. (1991). Neuroendocrinology 53, 503–510.
Sarkar, D. K. (1989). Neuroendocrinology 49, 520–524.
Ben-Jonathan, N., Mershon, J. L., Allen, D. L., and Steinmetz, R. W. (1996). Endocr. Rev. 17, 639–669.
Grattan, D. R., Pi, X. J., Andrews, Z. B., et al. (2001). Horm. Behav. 40, 115–124.
Milenkovic, L., D’Angelo, G., Kelly, P. A., and Weiner, R. I. (1994). Proc. Natl. Acad. Sci. USA 91, 1244–1247.
Weber, R. F. A. and Calogero, A. E. (1991). Neuroendocrinology 54, 248–253.
Pi, X. J. and Grattan, D. R. (1999). J. Mol. Endocrinol. 23, 13–22.
Pi, X. J. and Grattan, D. R. (1999). J. Neuroendocrinol. 11, 693–705.
Gosh, R. and Sladek, C. D. (1995). Am. J. Physiol. 269, E76-E84.
Pi, X.-J. and Grattan, D. R. (1998). J. Comp. Neurol. 394, 462–474.
Crowley, W. R. and Armstrong, W. E. (1992). Endocr. Rev. 13, 33–65.
Chiu, S., Koos, R. D., and Wise, P. M. (1992). Endocrinology 130, 1747–1749.
Rossi, N. F. (1998). Am. J. Physiol. 275, E687-E693.
Torner, L., Toschi, N., Nava, G., Clapp, C., and Neumann, I. D. (2002). Eur. J. Neurosci. 15, 1–11.
Clapp, C., Sears, P. S., and Nicoll, C. S. (1989). Endocrinology 125, 1054–1059.
Clapp, C., Sears, P. S., Russell, D. H., Richards, J., Levay-Young, B. K., and Nicoll, C. S. (1988). Endocrinology 122, 2892–2898.
Clapp, C., Martial, J. A., Guzman, R. C., Rentier-Delrue, F., and Weiner, R. I. (1993). Endocrinology 133, 1292–1299.
Corbacho, A. M., Nava, G., Eiserich, J. P., et al. (2000). J. Biol. Chem. 275, 13183–13186.
Clapp, C. and Weiner, R. I. (1992). Endocrinology 130, 1380–1386.
Bole-Feysot, C., Goffin, V., Edery, M., Binart, N., and Kelly, P. A. (1998). Endocr. Rev. 19, 225–268.
Clapp, C. (1987). Endocrinology 121, 2055–2064.
Royster, M., Driscoll, P., Kelly, P. A., and Freemark, M. (1995). Endocrinology 136, 3892–3900.
Sladek, C. D. and Knigge, K. M. (1977). Endocrinology 101, 411–420.
Samson W. K. (1985). Neuroendocrinology 40, 277–279.
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Mejía, S., Torner, L.M., Jeziorski, M.C. et al. Prolactin and 16K prolactin stimulate release of vasopressin by a direct effect on hypothalamo-neurohypophyseal system. Endocr 20, 155–161 (2003). https://doi.org/10.1385/ENDO:20:1-2:155
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DOI: https://doi.org/10.1385/ENDO:20:1-2:155