Skip to main content
SpringerLink
Log in

Biochemical evidence of the functional recovery and regeneration of adrenal autotransplants in the rat spleen

  • Published:
Endocrine Aims and scope Submit manuscript

Abstract

Adrenal transplantation may restore adrenal function after bilateral adrenalectomy or when adrenal function is lost. Thus, animal experiments could provide useful information before clinical application of adrenal transplantation. Using an experimental model of autotransplantion of a complete adrenal gland in the spleen of adrenalectomized rats, several biochemical and hormonal parameters were studied to evaluate the function of transplanted adrenal tissue compared to control and adrenalectomized animals. Three weeks after surgery, the animals were sacrificed and plasma and tissue samples were obtained for biochemical studies. In the autotransplanted rats, plasma glucose, hepatic glycogen, plasma, and hepatic proteins, which were decreased in adrenalectomized rats, increased to values close to those of the control group; whereas muscle and thymus proteins, which were increased in adrenalectomized animals, decreased and reached normal levels. Corticosterone plasma levels in autotransplanted rats showed a 50% recovery compared to control animals, whereas plasma aldosterone concentrations were low, with similar values to those of the adrenalectomized group. These results provide evidence that the adrenal grafts secrete corticosterone in quantities enough to overcome hepatic inactivation. On the other hand, aldosterone plasma concentrations remain very low, plasma potassium levels are increased, and plasma sodium levels are decreased in animals with intrasplenic adrenal grafts, indicating that aldosterone production is insufficient to avoid hypoaldosteronism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Belloni, A., Vassanelli, P., Robba, C., Rebuffat, P., Mazzocchi, G., and Nussdorfer, G. (1982). J. Anat. 135, 245–253.

    PubMed  CAS  Google Scholar 

  2. Belloni, A., Neri, G., Musajo, F., et al. (1990). Endocrinology 126, 3251–3262.

    PubMed  CAS  Google Scholar 

  3. Matsumoto, T. (1990). Jpn. J. Surg. 20, 307–315.

    Article  PubMed  CAS  Google Scholar 

  4. Nabishah, B., Khalid, B., Morat, P., and Zanariyah, A. (1998). Exp. Clin. Endocrinol. Diabetes 106, 419–424.

    PubMed  CAS  Google Scholar 

  5. Belloni, A., Neri, G., Andreis, P., Musajo, F., Boscaro, M., Mazzocchi, G., and Nussdorfer, G. (1991). Cell Tissue Res. 265, 35–41.

    Article  PubMed  CAS  Google Scholar 

  6. Rebuffat, P., Neri, G., Andreis, P., Belloni, A., Mazzocchi, G., Musajo, F., and Nussdorfer, G. (1991). Cytobios 65, 101–113.

    PubMed  CAS  Google Scholar 

  7. Ho, M. and Vinson, G. (1993). J. Endocrinol. 139, 301–306.

    Article  PubMed  CAS  Google Scholar 

  8. Ho, M., Barker, S., and Vinson, G. (1994). J. Endocrinol. 141, 459–466.

    PubMed  CAS  Google Scholar 

  9. Vendeira, P., Pignatelli, D., Neves, D., Magalhães, M. M., Magalhães, M. C., Ho, M., and Vinson, G. (1996). J. Endocrinol. 149, 497–502.

    Article  PubMed  CAS  Google Scholar 

  10. Vinson, G., Ho, M., and Puddefoot, J. (1998). Endocr. Res. 24, 677–686.

    Article  PubMed  CAS  Google Scholar 

  11. Teebken, O. and Scheumann, G. (2000). Transplantation 70, 836–843.

    Article  PubMed  CAS  Google Scholar 

  12. Ricordi, C., Santiago, J., and Lacy, P. (1987). Endocrinology 121, 745–748.

    Article  PubMed  CAS  Google Scholar 

  13. Erdogan, G., Kologlu, S., Kamel, N., Baskal, N., Cesur, V., and Eraslan, S. (1994). Endocr. J. 41, 45–48.

    PubMed  CAS  Google Scholar 

  14. Murthy, A. and Franklin, A. (1973). Horm. Res. 4, 219–223.

    PubMed  CAS  Google Scholar 

  15. Robba, C., Vassanelli, P., Mazzocchi, G., and Nussdorfer, G. (1984). Exp. Pathol. 25, 115–119.

    PubMed  CAS  Google Scholar 

  16. Ulrich-Lai, Y. and Engeland, W. (2000). J. Neuroendocrinol. 12, 881–893.

    Article  PubMed  CAS  Google Scholar 

  17. Vendeira, P., Magalhães, M. M., and Magalhães, M. C. (1992). Anat. Rec. 232, 262–272.

    Article  PubMed  CAS  Google Scholar 

  18. Vendeira, P., Neves, D., Magalhães, M. M., and Magalhães, M. C. (1996). Anat. Rec. 246, 98–106.

    Article  PubMed  CAS  Google Scholar 

  19. Wilkinson, C., Shinsako, J., and Dallman, M. (1981). Endocrinology 109, 162–169.

    Article  PubMed  CAS  Google Scholar 

  20. Engeland, C. (1984). Endocrinology 114, 2160–2166.

    PubMed  CAS  Google Scholar 

  21. Saxe, A. and Connors, M. (1985). Surgery 98, 995–999.

    PubMed  CAS  Google Scholar 

  22. Penney, D., Patt, D., and Dixon, W. (1963). Anat. Rec. 146, 319–335.

    Article  PubMed  CAS  Google Scholar 

  23. Bertholet, J. (1980). J. Endocrinol. 87, 1–9.

    PubMed  CAS  Google Scholar 

  24. Pudney, J., Price, G., Whitehouse, B., and Vinson, G. (1984). Anat. Rec. 210, 603–615.

    Article  PubMed  CAS  Google Scholar 

  25. Bradford, M. (1976). Anal. Biochem. 72, 248–254.

    Article  PubMed  CAS  Google Scholar 

  26. Murat, J. and Serfaty, A. (1974). Clin. Chem. 20, 1576–1577.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Biología de la Reproducción, Instituto Nacional de la Nutrición “Salvador Zubirán”, Vasco de Quiroga 15, Delegación Tlalpan, 14000, México, DF, México

    Roberto Chavira

  2. Depto. de Fisiología y Farmacología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, C. P. 20100, Aguascalientes, Ags., México

    Gonzalo Allende & Andrés Quintanar-Stephano

Authors
  1. Gonzalo Allende
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Roberto Chavira
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andrés Quintanar-Stephano
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gonzalo Allende.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Allende, G., Chavira, R. & Quintanar-Stephano, A. Biochemical evidence of the functional recovery and regeneration of adrenal autotransplants in the rat spleen. Endocr 16, 173–179 (2001). https://doi.org/10.1385/ENDO:16:3:173

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/ENDO:16:3:173

Key Words

Search

Navigation