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Differential effects of glutamate agonists and d-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats

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Abstract

In order to determine whether ionotropic (iGluRs) and metabotropic (mGluRs) glutamate receptor activation modulates oxytocin release in male rats, we investigated the effect of agonists of both types of glutamate receptors on oxytocin release from hypothalamus and posterior pituitary. Kainate and quisqualate (1 mM) increased hypothalamic oxytocin release. Their effects were prevented by selective AMPA/kainate receptor antagonists. NMDA (0.01–1 mM) did not modify hypothalamic oxytocin release. Group I mGluR agonists, such as quisqualate and 3-HPG, significantly increased hypothalamic oxytocin release. These effects were blocked by AIDA (a selective antagonist of group I mGluRs). In the posterior pituitary, oxytocin release was not modified by kainate, quisqualate, trans-ACPD (a broad-spectrum mGluR agonist) and l-SOP (a group III mGluR agonist). However, NMDA (0.1 mM) significantly decreased oxytocin release from posterior pituitary. d-Aspartate significantly increased oxytocin release from the hypothalamus, while it decreased oxytocin release from posterior pituitary. AP-5 (a specific NMDA receptor antagonist) reduced the d-Aspartate effect in the hypothalamus, but not in the posterior pituitary. Our data indicate that the activation of non-NMDA receptors and group I mGluRs stimulates oxytocin release from hypothalamic nuclei, whereas NMDA inhibits oxytocinergic terminals in the posterior pituitary. d-Aspartate also has a dual effect on oxytocin release: stimulatory at the hypothalamus and inhibitory at the posterior pituitary. These results suggest that excitatory amino acids differentially modulate the secretion of oxytocin at the hypothalamic and posterior pituitary levels.

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References

  1. Silverman, A. J. and Zimmerman, E. A. (1983). Annu. Rev. Neurosci. 6, 251–262.

    Article  Google Scholar 

  2. Ben Jonathan, N., Laudon, M., and Garris, P. (1991). Front. Neuroendocrinol. 12, 231–277.

    Google Scholar 

  3. Morris, J. F., Christian, H., Ma, D., and Wang, H. (2000). Exp. Physiol. 85S, 131S-138S.

    Article  Google Scholar 

  4. Page, W. K., King, W. M., Merigan, W., and Maunsell, J. (1994). Vision Res. 34, 223–239.

    Article  PubMed  CAS  Google Scholar 

  5. De Wied, D., Diamant, M., and Fodor, M. (1993). Front. Neuroendocrinol. 14, 251–302.

    Article  PubMed  Google Scholar 

  6. Engelmann, M., Wotjak, C. T., Neumann, I., Ludwig, M., and Landgraf, R. (1996). Neurosci. Biobehav. Rev. 20, 341–358.

    Article  PubMed  CAS  Google Scholar 

  7. Chalmers, J. and Pilowsky, P. (1991). J. Hypertens. 9, 675–694.

    Article  PubMed  CAS  Google Scholar 

  8. Huang, W., Lee, S. L., and Sjoquist, M. (1995). Am. J. Physiol. 268, R634-R640.

    PubMed  CAS  Google Scholar 

  9. Van del Pol, A. N., Wuarin, J. P., and Dudek, F. (1990). Science 250, 1276–1278.

    Article  PubMed  Google Scholar 

  10. Brann, D. W. and Mahesh, V. B. (1997). Endocrine Rev. 18, 678–700.

    Article  CAS  Google Scholar 

  11. Schoepp, D. D. and Conn, P. J. (1993). TIPS 14, 13–20.

    PubMed  CAS  Google Scholar 

  12. Meeker, R. B., Swanson, D. J., Greenwood, R. S., and Hayward, J. N. (1993). Brain Res. 600, 112–122.

    Article  PubMed  CAS  Google Scholar 

  13. El Majdoubi, M., Poulain, D. A., and Theodosis, D. T. (1996). Eur. J. Neurosci. 8, 1377–1398.

    Article  PubMed  Google Scholar 

  14. Morris, J. F., Pow, D. V., Sokol, H. W., and Ward, A. (1993). In: Vasopressin. Gross, P., Richter, D., and Robertson, G.L. (eds.). John Libbey Eurotext: Paris.

    Google Scholar 

  15. Ginsberg, S. D., Price, D. L., Blackstone, C. D., Huganir, R. L., and Martin, L. J. (1995). Neuroscience 65, 563–575.

    Article  PubMed  CAS  Google Scholar 

  16. Martin, D., Blackstone, C. D., Levey, A. I., Huganir, R. L., and Price, D. L. (1993). Neuroscience 53, 327–358.

    Article  PubMed  CAS  Google Scholar 

  17. Petralia, R. S. and Wenthold, R. J. (1992). J. Comp. Neurol. 318, 329–354.

    Article  PubMed  CAS  Google Scholar 

  18. Sato, K., Kiyama, H., and Tohyama, M. (1993). Neuroscience 52, 515–539.

    Article  PubMed  CAS  Google Scholar 

  19. Van del Pol, A. N., Herman-Borgmeyer, I., Hofer, M., Ghosh, P., and Heinemann, S. (1994). J. Comp. Neurol. 343, 428–444.

    Article  PubMed  Google Scholar 

  20. Sato, K., Kiyama, H., and Tohyama, M. (1995). Neuroscience 64, 459–475.

    Article  PubMed  CAS  Google Scholar 

  21. Meeker, R. B., Greenwood, R. S., and Hayward, J. N. (1994). Endocrinology 134, 621–629.

    Article  PubMed  CAS  Google Scholar 

  22. Van den Pol, A. N. (1994). J. Comp. Neurol. 349, 615–632.

    Article  PubMed  Google Scholar 

  23. Petralia, R. S., Wang, Y. X., Niedzielski, A. S., and Wenthold, R. J. (1996). Neuroscience 71, 949–976.

    Article  PubMed  CAS  Google Scholar 

  24. Lasaga, M., De Laurentiis, A., Pampillo, M., Pisera, D., Díaz, M., Theas, S., Duvilanski, B., and Seilicovich, A. (1998). Neurosc. Letts. 247, 119–122.

    Article  CAS  Google Scholar 

  25. Hattori, T., Sundberg, D. K., and Morris, M. (1992). Brain Res. Bull. 28, 257–263.

    Article  PubMed  CAS  Google Scholar 

  26. Moos, F. C., Rossi, K., and Richards, P. (1997). Neuroscience 77, 993–1002.

    Article  PubMed  CAS  Google Scholar 

  27. Parker, S. L. and Crowley, W. R. (1993). Endocrinology 133, 2847–2854.

    Article  PubMed  CAS  Google Scholar 

  28. Hashimoto, A., Nishikawa, T., Oka, T., Hayashi, T., and Takahashi, K. (1993). FEBS Lett. 27, 4–8.

    Article  Google Scholar 

  29. Schell, M. J., Cooper, O. B., and Snyder, S. H. (1997). Proc. Natl. Acad. Sci. USA 94, 2013–2018.

    Article  PubMed  CAS  Google Scholar 

  30. Bleakman, D. and Lodge, D. (1998). Neuropharmacology 37, 1187–1204.

    Article  PubMed  CAS  Google Scholar 

  31. Hu, B. and Bourque, C. W. (1992). J. Physiol. 458, 667–687.

    PubMed  CAS  Google Scholar 

  32. Petralia, R. S., Wang, Y. X., and Wenthold, R. J. (1994). J. Neurosci. 14, 6102–6120.

    PubMed  CAS  Google Scholar 

  33. Nissen, R., Hu, B., and Renaud, L. P. (1995). J. Physiol. London 484, 415–424.

    PubMed  CAS  Google Scholar 

  34. Nissen, R., Hu, B., and Renaud, L. P. (1994). Neuroscience 59, 115–120.

    Article  PubMed  CAS  Google Scholar 

  35. Stern, J. E., Galarreta, M., Foehring, R. C., Hestrin, S., and Armstrong, W. E. (1999). J. Neurosci. 19, 3367–3375.

    PubMed  CAS  Google Scholar 

  36. Morsette, D. J., Sidorowicz, H., and Sladek, C. (2001). Am. J. Physiol. Regulatory Integrative Comp. Physiol. 280, R313-R322.

    CAS  Google Scholar 

  37. Boudaba, C., Szabó, K., and Tasker, J. G. (1997). J. Neurophysiol. 77, 3396–3400.

    PubMed  CAS  Google Scholar 

  38. Richardson, C. M., and Wakerley, J. B. (1997). Brain Res. 767, 158–161.

    Article  PubMed  CAS  Google Scholar 

  39. Sun, Q., Pretel, S., Applegate, C. D., and Piekut, D. T. (1996). Neuroscience 71, 543–554.

    Article  PubMed  CAS  Google Scholar 

  40. Jourdain, P., Poulain, D. A., Theodosis, D. T., and Israel, J. M. (1996). J. Neurophysiol. 76, 2272–2285.

    Google Scholar 

  41. Schrader, L. A. and Tasker, J. G. (1997). J. Neurophysiol. 78, 3428–3437.

    PubMed  CAS  Google Scholar 

  42. Hashimoto, A. and Oka, T. (1997). Progr. Neurobiol. 52, 325–353.

    Article  CAS  Google Scholar 

  43. Lee, J., Homma, H., Tashiro, K., Iwatsubo, T., and Imai, K. (1999). Brain Res. 838, 193–199.

    Article  PubMed  CAS  Google Scholar 

  44. Long, Z., Homma, H., Lee, J., Fukushima, T., Santa, T., Iwatsubo, T., Yamada, R., and Imai, K. (1998). FEBS Lett. 434, 231–235.

    Article  PubMed  CAS  Google Scholar 

  45. D’Aniello, A., Difiore, M. M., Fisher, G. H., Milone, A., Seleni, A., D’Aniello, S., Perna, A. F., and Ingrosso, D. (2000). FASEB J. 14, 699–714.

    PubMed  CAS  Google Scholar 

  46. Wang, H., Wolosker, H., Pevsner, J., Snyder, S. H., and Selkoe, D. J. (2000). J. Endocrinol. 167, 247–252.

    Article  PubMed  CAS  Google Scholar 

  47. Al-Ghoul, W. M., Meeker, R. B., and Greenwood, R. S. (1997). Mol. Brain Res. 44, 262–272.

    Article  PubMed  CAS  Google Scholar 

  48. Wafford, K. A., Bain, C. J., Le Bourdelles, B., Whiting, P. J., and Kemp, J. A. (1993). Neuroreport 4, 1347–1349.

    Article  PubMed  CAS  Google Scholar 

  49. Nagata, Y., Homma, H., Lee, J., and Imai, K. (1999). FEBS Lett. 444, 160–164.

    Article  PubMed  CAS  Google Scholar 

  50. Di Fiore, M., Assisi, L., Botte, V., and D’Aniello, A. (1998). J. Endocrinol. 157, 199–207.

    Article  PubMed  Google Scholar 

  51. Kendrick, K. M. (2000). Exp. Physiol. 85S, 111S-124S.

    Article  Google Scholar 

  52. Richard, P., Moos, F. C., and Freund-Mercier, M. J. (1991). Physiol. Rev. 71, 331–370.

    PubMed  CAS  Google Scholar 

  53. Ludwig, M. (1998). J. Neuroendocrinology 10, 881–895.

    Article  CAS  Google Scholar 

  54. Pow, D. V. and Morris, J. F. (1989). Neuroscience 32, 435–439.

    Article  PubMed  CAS  Google Scholar 

  55. Pittman, Q. J., Hirasawa, M., Mouginot, D., and Kombian, S. B. (2000). Exp. Physiol. 85S, 139S-143S.

    Article  Google Scholar 

  56. De Laurentiis, A., Pisera, D., Duvilanski, B., Rettori, V., Lasaga, M., and Seilicovich, A. (2000). Brain Res. Bull. 53, 325–330.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Piso 10, 1121, Buenos Aires, Argentina

    Macarena Pampillo, María del Carmen Díaz, Beatriz H. Duvilanski, Adriana Seilicovich & Mercedes Lasaga

  2. Centro de Estudios Farmacológicos y Botánicos, Consejo Nacional de Investigaciones Científicas y Técnicas, 1414, Buenos Aires, Argentina

    Valeria Rettori

Authors
  1. Macarena Pampillo
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  2. María del Carmen Díaz
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  3. Beatriz H. Duvilanski
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  4. Valeria Rettori
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  5. Adriana Seilicovich
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  6. Mercedes Lasaga
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Correspondence to Mercedes Lasaga.

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Pampillo, M., del Carmen Díaz, M., Duvilanski, B.H. et al. Differential effects of glutamate agonists and d-aspartate on oxytocin release from hypothalamus and posterior pituitary of male rats. Endocr 15, 309–315 (2001). https://doi.org/10.1385/ENDO:15:3:309

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  • DOI: https://doi.org/10.1385/ENDO:15:3:309

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