Skip to main content
SpringerLink
Log in

Site-specific control of rat preadipocyte adipose conversion by ovarian status

Possible involvement of CCAAT/enhancer-binding protein transcription factors

  • Published:
Endocrine Aims and scope Submit manuscript

Abstract

The preadipocyte-adipocyte conversion process from two intraabdominal (parametrial and perirenal fat depots) is differently affected by ovarian status in the rat. We have tested the hypothesis that these sitespecific alterations of adipogenesis might be related to changes in the expression of the transcription factors c-myc and CCAAT/enhancer binding proteins (C/EBPα,-β, and-ζ) that regulate proliferation and differentiation. The increased proliferation rates observed in parametrial and perirenal preadipocytes after ovariectomy were not linked to variations in c-myc mRNA levels. Expression of the early marker of adipogenesis, lipoprotein lipase (LPL), remained insensitive to the ovarian status in early differentiated parametrial and perirenal preadipocytes. By contrast, LPL expression increased in early differentiated sc preadipocytes from ovariectomized rats, an, effect that was completely reversed by in vivo estradiol and progesterone treatment. Expression of C/EBPβ protein was unaffected by ovarian status whatever the anatomic origin of the preadipocytes. By contrast, the levels of p42 and p30 isoforms of C/EBPα were specifically decreased in parametrial preadipocytes, an alteration that was completely corrected by in vivo administration of estradiol and progesterone. C/EBPζ, a dominant inhibitor of C/EBPα and β, exhibited a strong site-specific expression since C/EBPζ content was fivefold higher in sc preadipocytes than in deep intraabdominal cells whatever the ovarian status. Furthermore, ovariectomy selectively decreased C/EBPζ levels in sc cells. In conclusion, our study suggests that some of the site-specific effects of ovariecaltered expressions of C/EBPα and ζ, both of which are important transcriptional regulators of fat cell differentiation and metabolism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Krotkiewski, M. (1976). Acta Physiol. Scand. 96, 128–133.

    Article  PubMed  CAS  Google Scholar 

  2. Hansjen, F. M., Fahmy, N., and Nielsen, J. H. (1980). Acta Endocrinol. 95, 566–570.

    Google Scholar 

  3. Pedersen, S. D., Borglum, J. D., Moller-Pedersen, T., and Richelsen, B. (1992). Mol. Cell. Endocrinol. 85, 13–19.

    Article  PubMed  CAS  Google Scholar 

  4. Rebuffe-Scrive, M., Enk, L., Crona, N., Lonnroth, P., Abrahamsson, L., Smith, U., and Bjorntorp, P. (1985). J. Clin. Invest. 75, 1973–1976.

    PubMed  CAS  Google Scholar 

  5. Simpson, E. R., Merill, J. C., Hollub, A. J., Graham-Lorence, S., and Mendelson, C. R. (1989). Endocr. Rev. 10, 136–148.

    PubMed  CAS  Google Scholar 

  6. Gray, J. M. and Wade, G. N. (1979). Endocrinology 104, 1377–1382.

    PubMed  CAS  Google Scholar 

  7. Gray, J. M., Dudley, S. D., and Wade, G. N. (1981). Am. J. Physiol. 240, E43-E46.

    PubMed  CAS  Google Scholar 

  8. Xu, X., Hoebeke, J., and Bjorntorp, P. (1990). J. Steroid Biochem. 36, 465–471.

    Article  PubMed  CAS  Google Scholar 

  9. O'Brien, S. N., Welter, B. H., Mantzke, K. A., and Price, T. M. (1998). J. Clin. Endocrinol. Metab. 83, 509–513.

    Article  PubMed  Google Scholar 

  10. Price, T. M. and O'Brien, S. N. (1993). J. Clin. Endocrinol. Metab. 77, 1041–1045.

    Article  PubMed  CAS  Google Scholar 

  11. Pedersen, S. B., Borglum, J. D., Eriksen, E. F., and Richelsen, B. (1991). Biochim. Biophys. Acta 1093, 80–86.

    Article  PubMed  CAS  Google Scholar 

  12. Jaubert, A. M., Pecquery, R., Dieudonné, M. N., Cloix, J. F., and Giudicelli, Y. (1995). Gen. Comp. Endocrinol. 100, 179–187.

    Article  PubMed  CAS  Google Scholar 

  13. Crandall, D. L., Busler, D. E., Novak, T. J., Weber, R. V., and Kral, J. G. (1998). Biochem. Biophys. Res. Commun. 248, 523–526.

    Article  PubMed  CAS  Google Scholar 

  14. Lacasa, D., Agli, B., Pecquery, R., and Giudicelli, Y. (1991). Endocrinology 128, 747–753.

    PubMed  CAS  Google Scholar 

  15. Lacasa, D., Garcia, E., Agli, B., and Giudicelli, Y. (1997). Endocrinology 138, 2729–2734.

    Article  PubMed  CAS  Google Scholar 

  16. Evans, G. I. and Littlewood, T. D. (1993). Curr. Opin. Genet. Dev. 3, 44–49.

    Article  Google Scholar 

  17. Amati, B. and Lands, H. (1994). Curr. Opin. Genet. Dev. 4, 102–108.

    Article  PubMed  CAS  Google Scholar 

  18. Schuchard, M., Landers, J. P., Sandhu, N. P., and Spelsberg, T. C. (1993). Endocr. Rev. 14, 659–669.

    Article  PubMed  CAS  Google Scholar 

  19. Murphy, L. J., Murphy, L. C., and Freisen, H. G. (1987). Endocrinology 120, 1882–1888.

    Article  PubMed  CAS  Google Scholar 

  20. Szijan, I., Parma, D. L., and Engel, N. I. (1992). J. Steroid Biochem. Mol. Biol. 40, 223–230.

    Google Scholar 

  21. Piontkewitz, Y., Sundfeld, K., and Hedin, L. (1997). J. Endocrinol. 152, 395–406.

    Article  PubMed  CAS  Google Scholar 

  22. Wong, M. S. J. and Murphy, L. C. (1991). J. Steroid Biochem. Mol. Biol. 39, 39–44.

    Article  PubMed  CAS  Google Scholar 

  23. Darlington, G. J., Ross, S. E., and Mac Dougald, O. A. (1998). J. Biol. Chem. 273, 30,057–30,060.

    Article  CAS  Google Scholar 

  24. Cao, Z., Umek, R. M., and McKnight, S. L. (1991). Genes Dev. 5, 1538–1552.

    Article  PubMed  CAS  Google Scholar 

  25. Christy, R. J., Yang, V. W., Ntambi, J. M., Geiman, D. E., Landchulz, W. H., Friedman, A. D., Nakabeppu, Y., Kelly, T. J., and Lane, D. (1989). Genes Dev. 3, 1323–1335.

    Article  PubMed  CAS  Google Scholar 

  26. Hwang, C. S., Mandrup, S., Mac Dougald, O. A., Geiman, D. E., and Lane, M. D. (1996). Proc. Natl. Acad. Sci. USA 93, 873–877.

    Article  PubMed  CAS  Google Scholar 

  27. Constance, C., Morgan, J., and Umek, R. (1993). Mol. Cell. Biol. 16, 3878–3883.

    Google Scholar 

  28. Timechenko, N., Wilde, M., Nakanishi, M., Smith, J., and Darlington, G. (1996). Genes Dev. 10, 804–815.

    Article  Google Scholar 

  29. Matsuno, F., Chowdhury, S., and Gotoh, T., Iwase, K., Matzsuzaki, K. Takatsuki, K., Mori, M., and Takiguchi, M. (1996). J. Biolchem. 119, 524–532.

    CAS  Google Scholar 

  30. Boudreau, F., Blais, S., and Asselin, C. (1996). Exp. Cell Res. 222, 1–9.

    Article  PubMed  CAS  Google Scholar 

  31. Mac Dougald, O. A., Cornelius, P., Lin, F. T., Chen, S. S., and Lane, M. D. (1994). J. Biol. Chem. 269, 19,041–19,047.

    CAS  Google Scholar 

  32. Piontkewitz, Y., Enerback, S., and Hedin, L. (1993). Endocrinology 133, 2327–2333.

    Article  PubMed  CAS  Google Scholar 

  33. Freytag, S. O. and Geddes, T. J. (1992). Science 256, 379–382.

    Article  PubMed  CAS  Google Scholar 

  34. Descombes, P. and Schibler, U. (1991). Cell 67, 569–579.

    Article  PubMed  CAS  Google Scholar 

  35. Mac Dougald, O. A. and Lane, M. D. (1995). Ann. Rev. Biochem. 64, 345–373.

    Article  CAS  Google Scholar 

  36. Ossipow, V., Descombes, P., and Schibler, U. (1993). Proc. Natl. Acad. Sci. USA 90, 8219–8223.

    Article  PubMed  CAS  Google Scholar 

  37. Mehmet, H., Littlewood, T. D., Sinnett-Smith, J., Moore, J. P., Evan, G. I., and Rozengurt, E. (1997). Cell Growth Diff. 8, 187–193.

    PubMed  CAS  Google Scholar 

  38. Band, C. J., Mounier, C., and Posner, B. I. (1999). Endocrinology 140, 5626–5634.

    Article  PubMed  CAS  Google Scholar 

  39. Cousin, B., Casteilla, L., Dani, C., Muzzin, P., Revelli, J. P., and Penicaud, L. (1993). Biochem. J. 292, 873–876.

    PubMed  CAS  Google Scholar 

  40. Garcia, E., Lacasa, M., Agli, B., Giudicelli, Y., and Lacasa, D. (1999). J. Endocrinol. 161, 89–97.

    Article  PubMed  CAS  Google Scholar 

  41. Wang, Y., Lee-Kwon, W., Martindale, J. L., Adams, L., Heller, P., Egan, J. E., and Bernier, M. (1999). Endocrinology 140, 2938–2947.

    Article  PubMed  CAS  Google Scholar 

  42. Prall, O. W. J., Sarcevic, B., Musgrove, E. A., Watts, C. K. V., and Sutherland, R. L. (1997). J. Biol. Chem. 272, 10,882–10,894.

    CAS  Google Scholar 

  43. Owen, G. K., Richer, J. K., Tung, L., Takimoto, G., and Horwitz, K. B. (1998). J. Biol. Chem. 273, 10,696–10,701.

    Article  CAS  Google Scholar 

  44. Santos, R. F., Sztalryd, C., and Reaven, G. (1991). Int. J. Obes. 15, 755–762.

    PubMed  CAS  Google Scholar 

  45. Ogawa, Y., Masuzaki, H., Isse, N., Okasaki, T., Mori, K., Shigemoto, M., Satoh, N., Tamura, N., Hosoda, K., Yoshimasa, Y., Jingami, H., Kawada, T., and Nakao, K. (1995). J. Clin. Invest. 96, 1647–1652.

    Article  PubMed  CAS  Google Scholar 

  46. Machinal, F., Dieudonné, M. N., Leneveu, M. C., Pecquery, R., and Giudicelli, Y. (1999). Endocrinology 140, 1567–1574.

    Article  PubMed  CAS  Google Scholar 

  47. Wang, X. Z., Kuroda, M., Sok, J., Batchuarova, N., Kimmel, R., Chung, P., Zinszner, H., and Ron, D. (1998). EMBO J. 17, 3629–3630.

    Google Scholar 

  48. Rosen, E. D., Walkey, C. J., Puigsever, P., and Spiegelman, B. M. (2000). Genes Dev. 14, 1293–1307.

    PubMed  CAS  Google Scholar 

  49. Horwitz, K. B., Jackson, T. A., Bain, D. L., Richer, J. F., Takimoto, G. S., and Tung, L. (1996). Mol. Endocrinol. 10, 1167–1177.

    Article  PubMed  CAS  Google Scholar 

  50. Misiti, E., Schonburg, L., Yen, P. M., and Chin, W. W. (1998). Endocrinology 139, 2493–2500.

    Article  PubMed  CAS  Google Scholar 

  51. La Borda, J. (1991). Nucl. Acid Res. 19, 3998.

    Article  Google Scholar 

  52. Nishimura, J., Kobayashi, S., Shikasho, T., and Kannaide, H. (1992). Biochem Biophys. Res. Commun. 188, 1198–1204.

    Article  PubMed  CAS  Google Scholar 

  53. Bradford, M. M. (1976). Anal. Biochem. 72, 248–254.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculté de Médecine Paris-Ouest, Université René Descartes and Laboratoire de Biochimie et Biologie Moléculaire, Centre Hospitalier de Poissy, Poissy, France

    Danièle Lacasa, Esther Garcia Dos Santos & Yves Giudicelli

Authors
  1. Danièle Lacasa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Esther Garcia Dos Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yves Giudicelli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Danièle Lacasa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lacasa, D., Santos, E.G.D. & Giudicelli, Y. Site-specific control of rat preadipocyte adipose conversion by ovarian status. Endocr 15, 103–110 (2001). https://doi.org/10.1385/ENDO:15:1:103

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/ENDO:15:1:103

Key Words

Search

Navigation