Abstract
A coculture of dermal pappilla cells (DPCs) from the balad frontal scalp of stumptailed macaques with keratinocytes derived from human neonatal foreskin revealed that testosterone inhibited keratinocyte proliferation, and that the antiandrogen RU58841 abolished this response. This testosterone-induced keratinocyte growth inhibition was not observed when either type of cells was cultured alone. We also examined conditioned media from the coculture system and demonstrated the identical testosterone-induced growth inhibition on keratinocytes, and this inhibitory effect was conditioned media concentration-dependent. These results suggested that the testosterone-mediated suppression on keratinocyte proliferation might proceed through some diffusible growth mediators in conditioned media. Differential display reverse transcriptase polymerase chain reaction allowed us to isolated several genes from frontal DPCs that can be either suppressed or induced by testosterone. Supervillin, a membrane-associated, F-actin-binding protein, was identified as one of the testosterone downregulated genes in frontal DPCs. Further characterization of these testosterone-target genesmay reveal the mechanism by which testosterone inhibits the growth of follicular cells in androgenetic alopecia.
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Cunha, G. R. (1972). Anat. Rec. 172(2), 179–195.
Chang, C., Kokontis, J., and Liao, S. (1988) Science 240, 324–326.
Chang, C., Kokontis, J., and Liao, S. (1988), Proc. Natl. Acad. Sci. USA 85, 7211–7215.
Chang, C., Kokontis, J., Swift, S., and Liao, S. (1990). Molecular endocrinology and steroid hormone action. Alan R. Liss, Inc.: pp. 53–63.
Cunha, G. R., Chung, L. W. K., Shannon, J. M., and Reese, B. A. (1980), Biol. Reprod. 22, 19–42.
Thompson, T. C., Cunha, G. R., Shannon, J. M., and Chung, L. W. K. (1986), J. Steroid Biochem. 25(5A), 627–634.
Itama, S., Kurata, S., Sonoda, T., and Takayasu, S. (1991), Ann. NY Acad. Sci. 642, 385–395.
Cunha, G. R., Chung, L. W. K., Shannon, J. M., Taguchi, O., and Fujii, H. (1983), Recent Prog. Horm. Res. 39, 559–598.
Cunha, G. R., Donjacour, A. A., Cooke, P. S., Mee, S., Bigsley, R. M., Higgins, S. J., and Sugimura, Y. (1987). Endocr. Rev. 8, 338–362.
Cooke, P. S., Young, P. G., and Cunha, G. R. (1987). Endocrinology 121(6), 2161–2170.
Swinnen, K., Cailleau, J., Heyns, W., and Verhoeven, G. (1990), Endocrinology 12(1), 142–150.
Chang, C., Saltzman, S., Yeh, S., Young, W., Keller, E., Lee, H., Wang, C., and Mizokami, A. (1995), Crit. Rev. Eukaryotic Gene Expression 5, 97–126.
Stumpf, W. E. and Sar, M. (1976). In: Modern pharmacology-toxicotology, 8, mechanism of action of steroid hormones, Pasqualini, J. (ed.), Marcel Dekker, New York.
Choudhry, R., Hodgins, M. B., Van der Kwast, T. H., Brinkmann, A. O., and Boersma, W. J. A. (1992), J. Endocrinol. 133, 467–475.
Inui, S., Itami, S., and Chang, C. (1999), J. of Investigative Dermatology in press.
Yeh, S., Chang, C. (1996), Proc. Natl. Acad. Sci. USA 93, 5517–5521.
Yeh, S., Miyamoto, H., and Chang, C. (1997), Lancet 349, 852–853.
Fujimoto, N., Yeh, S., Kang, H. Y., Inui, S., Chang, H. C., Mizokami, A., and Chang, C. (1999), J. Biol. Chem. 274, 8316–8321.
Kang, H. Y., Yeh, S., Fujimoto, N., and Chang, C. (1999), J. Biol. Chem. 274, 8570–8576.
Goldfoot, D. A., Slob, A. K., Sheffler, G., Robinson, J. A., Wiegand, S. J., and Cords, J. (1975), Arch. Sex Behav. 4, 547–560.
Rittmaster, R. S., Uno, H., Povar, M. L., Mellin, T. N., and Loriaux, D. L. (1987), J. Clin. Endocrinol. Metab. 65, 188–193.
Uno, H. (1987). In Models in dermatology. Maibach, H. I. and Low, N. J. (eds), S. Karger A. G.: Basel, Switzerland.
Uno, H., Alsum, P. B., Bauers, K., and de Waal, FBM. (1991). In: Primatology today Ehra, A. (ed.), Elsevier Science: Amsterdam.
Rohdes, L., Harper, J., Uno, H., Gaito, G., Audette-Arruda, J., Kurata, S., Berman, C., Primka, R., and Pikounis, B. (1994), J. Clin. Endocrinol. Metab. 79, 991–996.
Pan, H.-J., Wilding, G., Uno, H., Inui, S., Goldsmith, L., Messing, E., and Chang, C. (1998). Endocrine 9(1), 39–43.
Obana, N., Chang, C., and Uno, H. (1997), Endocrinology 138, 356–361.
Rubin, J. S., Peehlm, D. M., Chedidm, M., Alaridm, E. T., Cunha, G. R., Ron, D., and Aaronson, S. A. (1992), International symposium on biology of prostate growth. p. 14. National Institute of Health: Bethesda, MD.
Seslar, S. P., Nakamur, T., and Byers, S. W. (1993). Cancer Res. 53 (6), 1233–1238.
Chung, L. W. K., Li, W., and Gleave, M. (1992), J. Cell Biochem. 16H, 99–105.
Itami, S., Kurata, S., and Takayasu, S. (1995), Biochem. Biophys. Res. Commun. 212 (3), 988–994.
Gniadecki, R. (1998), Gen. Pharmaceuticals 30(5), 619–622.
Tanaka, A., Miyamoto, K., Minamino, N., Takeda, M., Sato, B., Matsuo, H., and Matsumoto, K. (1992), Proc. Natl. Acad. Sci. USA 89, 8928–8932.
Chung, L. W. K. (1995), Cancer Surveys 23, 33–42.
Burridge, K. and Chrzanowska-Wodnicka, M. (1996), Annu Rev. Cell Dev. Biol. 12, 463–518.
Pope, R. K., Pestonjamasp, K. N., Smith, K. P., Wulfkuhle, J. D., Strassel, C. P., Lawrence, J. B., and Luna, E. J. (1998). Genomics 52, 342–351.
Pestonjamasp, K. N., Pope, R. K., Wulfkuhle, J. D., and Luna, E. J. (1997), J. Cell Biol. 139 (5), 1255–1269.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989). In: Molecular cloning: a laboratory manual (2nd ed) Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY.
Chang, C., de Silva, S. L., Ideta, R., Le, Y., Yeh, S., and Burbach, J. P. H. (1994), Proc. Natl. Acad. Sci. USA 91, 6040–6044.
Liang, P. and Pardee, A. B. (1992), Science 257, 967–971.
Lin, T. and Chang, C. (1997), Proc. Natl. Acad. Sci. USA 94, 4988–4993.
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Pan, HJ., Uno, H., Inui, S. et al. Roles of testosterone in the growth of keratinocytes through bald frontal dermal papilla cells. Endocr 11, 321–327 (1999). https://doi.org/10.1385/ENDO:11:3:321
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DOI: https://doi.org/10.1385/ENDO:11:3:321