Skip to main content
SpringerLink
Log in

Rat ovarian interleukin-1α

Interleukin-1-dependent in vitro expression

  • Published:
Endocrine Aims and scope Submit manuscript

Abstract

Evidence exists supporting the possibility that intraovarian interleukin-1 (IL-1) may play an intermediary role in the periovulatory cascade. Although the existence of a mammalian intraovarian IL-1 system has been convincingly demonstrated, most efforts have focused on the possibility that the mammalian ovary is a site of IL-1b production, reception, and action. the objective of this study was to explore the possibility of ovarian IL-1a expression, characterize its pattern of expression by cultured ovarian cells, and study its hormonal regulation. The basal in vitro expression of IL-1a by cultured whole ovarian dispersates from immature rats increased spontaneously, reaching a peak (sixford increase over untreated controls) at 4 h. Treatment with an IL-1 receptor antagonist (IL-1RA), human chorionic gonadorropin, or IL-1b failed to attenuate the initial 4-h burst of IL-1a expression. By contrast, treatment of whole ovarian dispersates with IL-1b for 48 h resulted in significant upregulation of IL-1 a transcripts (60-fold increase). This IL-1b effect was completely blocked by cotreatment with IL-1RA, thereby suggesting mediation via a specific IL-1 receptor. The IL-1b effect proved to be protein biosynthesis and eicosanoid dependent, nitric oxide independent, and relatively specific in that it was not reproduced by a select series of other granulosa cell agonists.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ben-Shlomo, I. and Adashi, E. Y. (1994). Curr. Opin. Endocrinol. Diabetes. Curr. Sci. Ser. 3, 187–192.

    Article  Google Scholar 

  2. Brannstrom, M., Wang, L., and Norman, R. J. (1993). Endocrinology 132, 399–404.

    Article  PubMed  CAS  Google Scholar 

  3. Takehara, Y., Dharmarajan, A. M., Kaufman, G., and Wallach, E. E. (1994), Endocrinology 134, 1788–1793.

    Article  PubMed  CAS  Google Scholar 

  4. Peterson, C. M., Hales, H. A., Hatasaka, H. H., Mitchell, M. D., Rittenhouse, L., and Jones, K. P. (1993), Endocrinology 133, 2301–2306.

    Article  PubMed  CAS  Google Scholar 

  5. Simon, C., Tsafriri, A., Chun, S.-Y., Piquette, G. N., Dang, W., and Polan, M. L. (1994), Biol. Reprod., 51, 662–667.

    Article  PubMed  CAS  Google Scholar 

  6. Hurwitz, A., Ricciarelli, E., Botero, L., Rohan, R. M., Hernandez, E. R., and Adashi, E. Y. (1992), Endocrinology 129, 3427–3429.

    Google Scholar 

  7. Hurwitz, A., et al. (1992), J. Clin. Invest. 89, 1746–1754.

    PubMed  CAS  Google Scholar 

  8. Polan, M. L., Loukides, J. A., and Honig, J. (1994), Am. J. Obstet. Gynecol. 170, 1000–1007.

    PubMed  CAS  Google Scholar 

  9. Simon, C., Frances, A., Piquette, G., and Polan, M. L. (1994). Biol. Reprod. 50, 449–457.

    Article  PubMed  CAS  Google Scholar 

  10. Brannstrom, M., Norman, R. J., Seamark, R. F., and Robertson, S. A. (1994), Biol. Reprod. 50, 88–94.

    Article  PubMed  CAS  Google Scholar 

  11. Deyerle, K. L., Sims, J. E., Dower, S. K., and Bothwell, M. A. (1992), J. Immunol. 149, 1657–1665.

    PubMed  CAS  Google Scholar 

  12. Scherzer, W. J., Ruutiainen-Altman, K. S. L., Putowski, L. T., Kol, S., Adashi, E. Y., and Rohan, R. M. (1995), J. Soc. Gynecol. Invest. 3, 131–140.

    Article  Google Scholar 

  13. Wang, L.-J., Brannstrom, M., Cui, K.-H., Simula, A. P., Hart, R. P., Maddocks, S., and Norman, R. J. (1997), J. Endocrinol. 152, 11–17.

    Article  PubMed  CAS  Google Scholar 

  14. Kokia, E., Hurwitz, A., Ricciarelli, E., Tedeschi, C., Resnick, C. E., Mitchell, M. D., and Adashi, E. Y. (1992), Endocrinology 130, 3095–3097.

    Article  PubMed  CAS  Google Scholar 

  15. Brannstrom, M., Wang, L., and Norman, R. G. (1993), Biol. Reprod. 48, 165–171.

    Article  PubMed  CAS  Google Scholar 

  16. Kokia, E., Hurwitz, A., Ben-Shlomo, I., Adashi, E. Y., and Yanagishita, M. (1993), Endocrinology 133, 2391–2394.

    Article  PubMed  CAS  Google Scholar 

  17. Hurwitz, A., Dushnik, M., Solomon, H., Ben-Shitrit, A., Finci-Yeheskel, Z., Milwidsky, A., Meyer, M., Adashi, E. Y., and Yagel, S. (1993), Endocrinology 132, 2709–2714.

    Article  PubMed  CAS  Google Scholar 

  18. Hurwitz, A., Finci-Yeheskel, Z., Sushnik, M., Milwidsky, A., Ben-Chetrit, A., Yagel, S., Adashi, E. Y., and Mayer, M. (1994), Mol. Cell. Endocrinol. 101, 307–315.

    Article  PubMed  CAS  Google Scholar 

  19. Bonello, N. P., Norman, R. J., and Brannstrom, M. (1995). Endocrine 3, 49–54.

    CAS  Google Scholar 

  20. Ellman, C., Corbett, J. A., Misko, T. P., McDaniel, M., and Beckerman, K. P. (1993), J. Clin. Invest. 92, 3053–3056.

    Article  PubMed  CAS  Google Scholar 

  21. Ben-Shlomo, I., Adashi, E. Y., and Payne, D. W. (1994), J. Clin. Invest. 94, 1463–1469.

    PubMed  CAS  Google Scholar 

  22. Ben-Shlomo, I., Kokia, E., Jackson, M., Adashi, E. Y., and Payne, D. W. (1994), Biol. Reprod. 51, 310–318.

    Article  PubMed  CAS  Google Scholar 

  23. Bonello, N., McKie, K., Jasper, M., Andre, L., Ross, N., Braybon, E., Brannstrom, M., and Norman, R. J. (1996), Biol. Reprod. 54, 436–445.

    Article  PubMed  CAS  Google Scholar 

  24. Chun, S.-Y., Eisenhauer, K. M., Kubo, M., and Hsueh, A. J. (1995), Endocrinology 136, 3120–3127.

    Article  PubMed  CAS  Google Scholar 

  25. Nappi, R. E., Guo, A. L., Petraglia, F., Bonati, M. E., Crisculolo, M., Ficarra, G., Zara, C., and Genazzani, A. R. (1994), Gynecol. Endocrinol. 8, 259–264.

    PubMed  CAS  Google Scholar 

  26. Sjogren, A., Holmes, P. V., and Hillensjo, T. (1991), Hum. Reprod. 6, 910–913.

    PubMed  CAS  Google Scholar 

  27. Hurwitz, A., Payne, D. W., Packman, J. N., Andreani, C. L., Resnick, C. E., Hernandez, E. R., and Adashi, E. Y. (1991). Endocrinology 129, 1250–1256.

    Article  PubMed  CAS  Google Scholar 

  28. Kasson, B. G. and Gorospe W. C. (1989), Mol. Cell. Endocrinol. 62, 103–111.

    Article  PubMed  CAS  Google Scholar 

  29. Espey, L. L. (1980), Biol. Reprod. 22, 73–106.

    Article  PubMed  CAS  Google Scholar 

  30. Dalton, B. J., Connor, J. R., and Johnson, W. J. (1989), Arthritis Rheum. 32, 279–287.

    Article  PubMed  CAS  Google Scholar 

  31. Sims, J. E., et al. (1993), Proc. Natl. Acad. Sci. USA 90, 6155–6159.

    Article  PubMed  CAS  Google Scholar 

  32. Colotta, F., et al. (1993), Science 261, 472–475.

    Article  PubMed  CAS  Google Scholar 

  33. Shukovski, L. and Tsafriri, A. (1994), Endocrinology 135, 2287–2290.

    Article  PubMed  CAS  Google Scholar 

  34. Hannun, Y. A. and Bell, R. M. (1989), Science 243, 500–507.

    Article  PubMed  CAS  Google Scholar 

  35. Zhang, Y. and Kolesnick, R. (1995), Endocrinology 136, 4157–4159.

    Article  PubMed  CAS  Google Scholar 

  36. Erickson, G. F., Magoffin, D. A., Dyer, C. A., and Hofeditz, C. (1985), Endocr. Rev. 6, 371–399.

    Article  PubMed  CAS  Google Scholar 

  37. Kol, S., Ben-Shlomo, I., Adashi, E. Y., and Rohan, R. M. (1996), Genetic Anal.: Biomol. Eng. 12, 129–132.

    Article  CAS  Google Scholar 

  38. Lowe, J. W. L., Roberts, J. C. T., Lasky, S. R., and LeRoith, D. (1987), Proc. Natl. Acad. Sci. USA 84, 8946–8950.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Kenneth H. H. Wong

    Present address: Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, 546 Chipeta Way, Room 109, 84108, Salt Lake City, UT

  2. Shahar Kol

    Present address: Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel

  3. Motomo Ando

    Present address: Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Tokyo, Japan

  4. Izhar Ben-Shlomo

    Present address: Department of Obstetrics and Gynecology, Haemek Medical Center, Afula, Israel

Authors and Affiliations

  1. Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore, MD

    Shahar Kol, Kenneth H. H. Wong, Motomo Ando, Izhar Ben-Shlomo &  Eli Y. Adashi

Authors
  1. Shahar Kol
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kenneth H. H. Wong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Motomo Ando
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Izhar Ben-Shlomo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Eli Y. Adashi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eli Y. Adashi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kol, S., Wong, K.H.H., Ando, M. et al. Rat ovarian interleukin-1α. Endocr 11, 269–275 (1999). https://doi.org/10.1385/ENDO:11:3:269

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/ENDO:11:3:269

Key Words

Search

Navigation