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Expression of prolactin receptor mRNA is increased in the preoptic area of lactating rats

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Abstract

This study investigated expression of prolactin receptor (PRL-R) mRNA in the preoptic area in midlactating rats compared with diestrous rats. Tissues from specific nuclei were micropunched from 300-μm thick rat brain sections with 300- or 500-μm diameter needles. After total RNA was extracted, the two forms of PRL-RmRNA were evaluated by reverse transcriptase polymerase chain reaction and Southern hybridization. The results showed that levels of long-form PRL-R mRNA in the ventrolateral preoptic nucleus and latero-anterior nucleus in lactating rats were significantly higher (p<0.05) than in diestrous rats. The ventromedial and medial preoptic nuclei in lactating rats also expressed moderately high levels of long-form mRNA when compared with (p=0.0547) diestrous rats. The ventromedial and ventrolateral preoptic nuclei, and ventrolateral hypothalamic nucleus in lactating rats expressed significantly higher levels of short-form mRNA than in diestrous rats. The increased expression of both forms of PRL-R mRNA helps explain numerous effects of PRL on brain functions during lactation.

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Authors and Affiliations

  1. Department of Anatomy and Structural Biology, School of Medical Sciences and Neuroscience Research Centre, University of Otago, PO Box 913, Dunedin, New Zealand

    Xiujun Pi & David R. Grattan

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  1. Xiujun Pi
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  2. David R. Grattan
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Correspondence to David R. Grattan.

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Pi, X., Grattan, D.R. Expression of prolactin receptor mRNA is increased in the preoptic area of lactating rats. Endocr 11, 91–98 (1999). https://doi.org/10.1385/ENDO:11:1:91

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  • DOI: https://doi.org/10.1385/ENDO:11:1:91

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