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Inhibition of hypothalamic GnRH secretion in the ewe by antigonadotropic decapeptide during the estrous cycle and nonbreeding season

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Abstract

Previous experiments from our laboratory and others have shown that the peptide antigonadotropic decapeptide (AGD) has marked inhibitory effects on luteinizing hormone (LH) secretion in rats and ewes. The first objective of this study was to determine whether AGD inhibits LH secretion by regulating hypothalamic release of gonadotropin hormone (GnRH). AGD (200 μg in 200 μL of 0.3% bovine serum albumin [BSA] saline) or vehicle was infused into the lateral ventricle of ovariectomized (OVX) ewes with hypophyseal-portal cannulae, and GnRH secretion was monitored. The frequency of GnRH and LH pulses in AGD-treated ewes was significantly decreased (p<0.05) but did not change in the control ewes. The second objective of this investigation was to evaluate changes in hypothalamic sensitivity to AGD in the ewe during the estrous cycle and nonbreeding season. During the estrous cycle, the effects of AGD on LH secretion were assessed following ovariectomy, during the metestrous, diestrous, and proestrous phases of the estrous cycle. The response to AGD during the estrous cycle was compared to its effect during the anestrous season. LH, cortisol, and prolactin (PRL) concentrations were assayed in peripheral blood samples obtained at 10-min intervals over a 6-h period prior to injection of either vehicle (200 μL of 0.3% BSA in 0.9% saline) or AGD (200 μg in 200 μL of vehicle), and for an additional 10 h following treatment. LH pulse frequency decreased after treatment with AGD (p<0.05) at all times in OVX and intact ewes compared to vehicle-treated controls. During the anestrous season, AGD treatment was more effective in inhibiting LH pulse frequency than during the breeding season (p<0.05). Furthermore, there was a significant increase (p<0.05) in mean cortisol concentrations after AGD infusion in all AGD-treated groups compared to controls independent of season or reproductive status. PRL concentrations were also increased (p<0.05) following treatment with AGD. These results suggest that inhibition of pulsatile LH release induced by AGD is modulated by alterations in frequency of hypothalamic discharges of GnRH. Furthermore, changes in the inhibitory actions of AGD may contribute to the seasonal regulation of hypothalamic GnRH secretion in the ewe.

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References

  1. Karsch, F. J., Dahl, G. E., Evans, N. P., Manning, J. M., Mayfield, K. P., Moenter, S. M., and Foster, D. L. (1993). Biol. Reprod. 49, 1377–1383.

    Article  PubMed  CAS  Google Scholar 

  2. Karsch, F. J., Bittman, E. L., Foster, D. L., Goodman, R. L., Legan, S. J., and Robinson, J. E. (1984). Recent Prog. Horm. Res. 40, 185–225.

    PubMed  CAS  Google Scholar 

  3. Goodman, R. L., Legan, S. J., Ryan, K. D., Foster, D. L., and Karsch, F. J. (1981). J. Endocrinol. 889, 229–240.

    Article  Google Scholar 

  4. Kennaway, D. J., Dunstan, E. A., Gilmore, T. A., and Seamark, R. F. (1984). J. Endocrinol. 102, 199–207.

    PubMed  CAS  Google Scholar 

  5. Bittman, E. L., Kaynard, A. H., Olster, D. H., Robinson, J. E., Yellon, S. M., and Karsch, F. J. (1985). Neuroendocrinology. 40, 409–418.

    PubMed  CAS  Google Scholar 

  6. Viguie, C., Thibault, J., Thiery, J. C., Tillet, Y., and Malpaux, B. (1997). Endocrinology 138, 499–506.

    Article  PubMed  CAS  Google Scholar 

  7. Arendt, J. (1986). Oxford Rev. Reprod. Biol. 8, 266–320.

    CAS  Google Scholar 

  8. Woodfill, C. J. I., Wayne, N. L., Moenter, S. M., and Karsch, F. J. (1994). Biol. Reprod. 50, 965–976.

    Article  PubMed  CAS  Google Scholar 

  9. Barrel, B. A., Moenter, S. M., Caraty, A., and Karsch, F. J. (1992). Biol. Reprod. 46, 1130–1135.

    Article  Google Scholar 

  10. Martin, G. B., Scaramuzzi, R. T., and Henstridge, J. D. (1983). J. Endocrinol. 96, 181–193.

    PubMed  CAS  Google Scholar 

  11. Goodman, R. L., Bittman, E. L., Foster, D. L., and Karsch, F. J. (1982). Biol. Reprod. 27, 580–589.

    Article  PubMed  CAS  Google Scholar 

  12. Martin, G. B. (1984). Biol. Rev. 59, 1–87.

    Article  PubMed  CAS  Google Scholar 

  13. Meyer, S. L. and Goodman, R. L. (1985). Endocrinology 116, 2054–2061.

    PubMed  CAS  Google Scholar 

  14. Havern, R. L., Whisnant, C. S., and Goodman, R. L. (1994). Endocrinology 134, 1905–1914.

    Article  PubMed  CAS  Google Scholar 

  15. Goodman, R. L., Havern, R. L., and Whisnant, C. S. (1996). Biol. Reprod. 54, 380–386.

    Article  PubMed  CAS  Google Scholar 

  16. Tortonese, D. J. and Lincoln, G. A. (1994). J. Endocrinol. 143, 25–32.

    PubMed  CAS  Google Scholar 

  17. Meyer, S. L. and Goodman, R. L. (1986). Biol. Reprod. 35, 562–571.

    Article  PubMed  CAS  Google Scholar 

  18. Corre, S. L. and Chemineau, P. (1993). J. Reprod. Fert. 97, 367–373.

    Article  Google Scholar 

  19. Benson, B., Ebels, I., and Hruby, V. J. (1990). In: Advances in pineal research, vol. 4. Reiter, R. J., and Lukaszyk, A. (eds.). John Libbey and Co., London.

    Google Scholar 

  20. Benson, B. and Ebels, I. (1994). Life Sci. 54, 437–443.

    Article  Google Scholar 

  21. Benson, B. and Machen, N. (1994). Life Sci. 55, 263–268.

    Article  PubMed  CAS  Google Scholar 

  22. Benson, B., Machen, N., Dunn, A. M., and Wise, M. E. (1996). Life Sci. 58, 1083–1090.

    Article  PubMed  CAS  Google Scholar 

  23. Benson, B. and Chawla, M. (1992). In: Program of the 9th international congress of endocrinology. Nice, France.

  24. Dunn, A. M., Benson, B., Esquivel, E., and Wise, M. E. (1993). Biol. Reprod. 48, A132.

    Google Scholar 

  25. Viguie, C., Caraty, A., Locatelli, A., and Malpaux, B. (1995). Biol. Reprod. 52, 1114–1120.

    Article  PubMed  CAS  Google Scholar 

  26. Viguie, C., Caraty, A., Locatelli, A., and Malpaux, B. (1995). Biol. Reprod. 52, 1156–1161.

    Article  PubMed  CAS  Google Scholar 

  27. Kao, C., Schaeffer, D. J., and Jackson, G. K. (1992). Biol. Reprod. 46, 425–434.

    Article  PubMed  CAS  Google Scholar 

  28. Whisnant, C. S., and Goodman, R. L. (1990). Biol. Reprod. 42, 656–661.

    Article  PubMed  CAS  Google Scholar 

  29. Calogero, A. E., Bernardini, R., Margioris, A. N., Bagdy, G., Gallucci, W. T., Munson, P. J., Tamarkin, L., Tomai, T. P., Brady, L., Gold, P. W., and Chrousos, G. P. (1989). Peptides 10, 189–200.

    Article  PubMed  CAS  Google Scholar 

  30. Feldman, S. and Weidenfeld, J. (1995). Neurosci. Lett. 198, 143–145.

    Article  PubMed  CAS  Google Scholar 

  31. Weiner, R. J. and Ganong, W. F. (1978). Physiol. Rev. 58, 905–973.

    PubMed  CAS  Google Scholar 

  32. Martire, M., Pistritto, G., and Preziosi, P. (1989). J. Neural. Trans. 78, 109–120.

    Article  CAS  Google Scholar 

  33. Thomas, G. B., Cummins, J. T., and Clark, I. J. (1988). Neurosci. Lett. 85, 143–146.

    Article  PubMed  CAS  Google Scholar 

  34. Costa, A., Trainer, P., and Grossman, A. (1993). Brain Res. 605, 187–192.

    Article  PubMed  CAS  Google Scholar 

  35. Pozzoli, G., Mancuso, C., Mirtella, A., Preziosi, P., Grossman, A. B. and Navarra, P. (1994). Endocrinology 135, 2314–2317.

    Article  PubMed  CAS  Google Scholar 

  36. Bonavera, J. J., Kalra, P. S., and Kalra, S. P. (1996). Endocrinology 137, 956–1062.

    Article  Google Scholar 

  37. Snyder, S. H. (1992). Science 257, 494–496.

    Article  PubMed  CAS  Google Scholar 

  38. Verma, A., Hirsh, D. J., Glatt, C. E., Ronnett, G. V., Snyder, S. H. (1993). Science 259, 381–384.

    Article  PubMed  CAS  Google Scholar 

  39. Pu, S., Xu, B., Kalra, S. P., and Kalra, P. S. (1996). Endocrinology 137, 1949–1955.

    Article  PubMed  CAS  Google Scholar 

  40. Lamar, C. A., Mahesh, V. B., and Brann, D. W. (1996). Endocrinology 137, 790–793.

    Article  PubMed  CAS  Google Scholar 

  41. Lincoln, G. A. and Wu, F. C. W. (1991). Neuroendocrinology 3, 309–317.

    CAS  Google Scholar 

  42. Hui, Y., Hastings, M. H., Maywood, E. S., and Ebling, F. J. P. (1992). J. Reprod. Fertil. 95, 935–945.

    Article  PubMed  CAS  Google Scholar 

  43. Mori, Y., Takeuchi, Y., Shimada, M., Hayashi, S., and Hoshino, K. (1990). Jpn. J. Vet. Sci. 52, 339–349.

    CAS  Google Scholar 

  44. Baldwin, B. A., Heavens, R. P., Kendrick, K. M., Parrott, R. F., Sharman, D. F., and Thornton, S. N. (1985). Proc. Physiol. Soc., Abstract 9P.

  45. Clarke, I. J. and Cummins, J. T. (1982). Endocrinology 111, 1737–1739.

    Article  PubMed  CAS  Google Scholar 

  46. Wise, M. E. (1990). Biol. Reprod. 43, 719–725.

    Article  PubMed  CAS  Google Scholar 

  47. Nett, T. M. and Adams, T. E. (1977). Endocrinology 101, 1135–1144.

    PubMed  CAS  Google Scholar 

  48. Niswender, G. D., Reichert, G. E. Jr., Midgley, A. R. Jr., and Nalbandov, A. V. (1969). Endocrinology 84, 1166–1173.

    PubMed  CAS  Google Scholar 

  49. Veldhuis, J. D., and Johnson, M. L. (1986). Am. J. Physiol. 250, E486-E493.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Animal Sciences, University of Arizona, Shantz 228, 85721, Tucson, AZ

    Hsu Lee, Eduardo Esquivel & Mark E. Wise

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  1. Hsu Lee
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  2. Eduardo Esquivel
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  3. Mark E. Wise
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Correspondence to Mark E. Wise.

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Lee, H., Esquivel, E. & Wise, M.E. Inhibition of hypothalamic GnRH secretion in the ewe by antigonadotropic decapeptide during the estrous cycle and nonbreeding season. Endocr 11, 75–82 (1999). https://doi.org/10.1385/ENDO:11:1:75

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  • DOI: https://doi.org/10.1385/ENDO:11:1:75

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