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Homologous upregulation of GnRH receptor mRNA by continuous GnRH in cultured rat pituitary cells

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Abstract

The present study examined the effects of continuous treatment with gonadotropi-releasing hormone (GnRH) on GnRH receptor (GnRH-R) mRNA levels in dispersed cultures of rat pituitary cells. Pituitary GnRH-R mRNA levels were determined by competitive reverse transcriptase polymerase chain reaction. When pituitary cells were continuously exposed to a low dose of GnRH (0.2 nM), GnRH-R mRNA levels were transiently increased. The levels of GnRH-R mRNA were significantly increased up to 6 h and diminished to untreated levels by 24 h. Luteinizing hormone (LH) release was also increased significantly up to 12 h, maintaining similar levels in LH release therafter. When GnRH antagonist([d-pGlu1, d-Phe2, d-Trp3.6]-LH-RH) was added to the cultures together with GnRH (0.2 nM) for 6 h, the stimulatory effect of GnRH on GnRH-R mRNA levels and LH release was significantly diminished in a dose-related manner. In another experiment, pituitary cells were treated with various doses of GnRH (0.02–200 nM) for a relatively short (6 h) or a longer (24 h) period. When pituitary cells were exposed for 6 h, all doses of GnRH 0.02–200 nM) significantly increased GnRH-R mRNA levels in a dose-dependent manner. By contrast, continuous exposure to GnRH for 24 h was ineffective in changing pituitary GnRH-R mRNA levels at any given doses. These results indicate that the duration of GnRH treatment is critical for upregulation of GnRH-R mRNA by continuous GnRH. When pituitary cells were treated for 6 h with either a continuous mode of GnRH (0.2 nM) or an hourly pulsatile mode of GnRH (0.2 nM, 6 mi/h), both treatment significantly augmented GnRH-R mRNA levels. Thus, the modes of GnRH application, if treated for a relatively short period, do not appear to make a significant difference in upregulation of GnRH-R mRNA levels. Collectively, our data provide strong evidence that continuous GnRH application is able to upregulate pituitary GnRH-R mRNA levels, if treated for a relatively short period (6 h).

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Authors and Affiliations

  1. Endocrine Laboratory, Medical Research Center, Yousei University, 120-752, Seul, South Korea

    Minseok Cheon, Deokbae Park & Kyungza Ryu

  2. Department of Pharmacology, College of Medicine, Yousei University, 120-752, Seoul, South Korea

    Kyungza Ryu

  3. Department of Molecular Biology, College of Natural Sciences, Seoul National University, 151-752, Seoul, South Korea

    Kyungjin Kim & Sang Dai Park

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  1. Minseok Cheon
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  2. Deokbae Park
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  3. Kyungjin Kim
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  4. Sang Dai Park
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Correspondence to Kyungza Ryu.

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Cheon, M., Park, D., Kim, K. et al. Homologous upregulation of GnRH receptor mRNA by continuous GnRH in cultured rat pituitary cells. Endocr 11, 49–55 (1999). https://doi.org/10.1385/ENDO:11:1:49

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  • DOI: https://doi.org/10.1385/ENDO:11:1:49

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