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Androgen receptor mRNA expression in the rhesus monkey ovary

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Abstract

Immunocytochemical detection of androgen receptors (ARs) in several compartments of the macaque ovary, including the germinal epithelium, follicle, and corpus luteum, suggests a role for androgens in modulating ovarian function via the classical receptor-mediated pathway. To examine AR mRNA expression in the rhesus monkey ovary, total RNA was isolated from whole ovaries, the germinal epithelium-enriched cortical and medullary compartments of the ovary, and corpora lutea from early (d 3–5), mid (d 6–8), midlate (d 10–12), and late (d 13–15) stages of the luteal phase of the menstrual cycle. RNA was also obtained from luteinized granulosa cells from monkeys receiving gonadotropin treatment to stimulate the development of multiple ovarian follicles. After reverse transcription of total RNA using oligo-dT as a primer, polymerase chain reaction (PCR) was used to amplify a unique 329 bp segment of the monkey AR hormone-binding region. Reverse transcriptase (RT)-PCR products of the expected size were detected in all ovarian and control tissues. Sequence analysis of the AR cDNA from the macaque ovary revealed 99% nucleotide homology and 100% predicted amino acid homology to the cDNA for the hormone-binding region of human AR. Northern analysis demonstrated the presence of a major AR mRNA species at 9.5 kb in corpus luteum, luteinized granulosa cells, and prostate, with additional bands detected in the corpus luteum and prostate at 7.9 and 3.4 kb, respectively. A sensitive RNase protection assay was used to examine AR mRNA levels in ovarian tissues and showed AR mRNA expression throughout the life-span of the corpus luteum. Thus, detection of AR mRNA in the primate ovary, including the periovulatory follicle and corpus luteum, supports the concept that these tissues are targets for receptor-mediated androgen action during the menstrual cycle.

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References

  1. Hillier, S. G. (1985). Oxford Rev. Reprod. Biol. 7, 168–222.

    CAS  Google Scholar 

  2. Sanders, S. L. and Stouffer, R. L. (1997). Biol. Reprod. 56, 1077–1087.

    Article  PubMed  CAS  Google Scholar 

  3. Ohara, A., Mori, T., Taii, S., Ban, C., and Narimoto, K. (1987). J. Clin. Endocrinol. Metab. 65, 1192–1200.

    PubMed  CAS  Google Scholar 

  4. Chaffin, C. L., Hess, D. L., and Stouffer, R. L. (1999). Hum. Reprod. 14, 642–649.

    Article  PubMed  CAS  Google Scholar 

  5. McNatty, K. P., Smith, D. M., Makris, A., Osathanondh, R., and Ryan, K. J. (1979). J. Clin. Endocrinol. Metab. 49, 851–860.

    PubMed  CAS  Google Scholar 

  6. Morgan, P. M., Boatman, D. E., and Bavister, B. D. (1990). Mol. Reprod. Dev. 27, 145–151.

    Article  PubMed  CAS  Google Scholar 

  7. Sanders, S. L., Stouffer, R. L., and Brannian, J. D. (1996). J. Clin. Endocrinol. Metab. 81, 591–596.

    Article  PubMed  CAS  Google Scholar 

  8. Simone, D. A., Chorich, L. P., and Mahesh, V. B. (1993). Biol. Reprod. 49, 1190–1201.

    Article  PubMed  CAS  Google Scholar 

  9. Schreiber, J. R., Reid, R., and Ross, G. T. (1976). Endocrinology 98, 1206–1213.

    PubMed  CAS  Google Scholar 

  10. Milwidsky, A., Younes, M. A., Besch, N. F., Besch, P. K., and Kaufman, R. H. (1980). Am. J. Obstet. Gynecol. 138, 93–98.

    PubMed  CAS  Google Scholar 

  11. Hild-Petito, S., West, N. B., Brenner, R. M., and Stouffer, R. L. (1991). Biol. Reprod. 44, 561–568.

    Article  PubMed  CAS  Google Scholar 

  12. Horie, K., Takakura, K., Fujiwara, H., Suginami, H., Liao, S., and Mori, T. (1992). Hum. Reprod. 7, 184–190.

    PubMed  CAS  Google Scholar 

  13. Suzuki, T., Sasano, H., Kimura, N., Tamura, M., Fukaya, T., Yajima, A., and Nagura, H. (1994). Hum. Reprod. 9, 1589–1595.

    PubMed  CAS  Google Scholar 

  14. Hirai, M., Hirata, S., Osada, T., Hagihara, K., and Kato, J. (1994). J. Steroid Biochem. Mol. Biol. 49, 1–7.

    Article  PubMed  CAS  Google Scholar 

  15. Tetsuka, M. and Hillier, S. G. (1997). J. Steroid Biochem. Mol. Biol. 61, 233–239.

    PubMed  CAS  Google Scholar 

  16. Hillier, S. G., Tetsuka, M., and Fraser, H. M. (1997). Hum. Reprod. 12, 107–111.

    Article  PubMed  CAS  Google Scholar 

  17. Weil, S. J., Vendola, K., Zhou, J., Adesanya, O. O., Wang, J., Okafor, J., and Bondy, C. A. (1998). J. Clin. Endocrinol. Metab. 83, 2479–2485.

    Article  PubMed  CAS  Google Scholar 

  18. Abdelgadir, S. E., Roselli, C. E., Choate, J. V. A., and Resko, J. A. (1999). Biol. Reprod. 60, 1251–1256.

    Article  PubMed  CAS  Google Scholar 

  19. Lubahn, D. B., Joseph, D. R., Sar, M., Tan, J., Higgs, H., Larson, R. E., French, F. S., and Wilson, E. M. (1988). Mol. Endocrinol. 2, 1265–1275.

    PubMed  CAS  Google Scholar 

  20. Duffy, D. M. and Stouffer, R. L. (1995). Endocrinology 136, 1869–1876.

    Article  PubMed  CAS  Google Scholar 

  21. Tetsuka, M., Whitelaw, P. F., Bremner, W. J., Millar, M. R., Smyth, C. D., and Hillier, S. G. (1995). J. Endocrinol. 145, 535–543.

    Article  PubMed  CAS  Google Scholar 

  22. Liao, S. and Fang, S. (1969). Vitam. Horm. 27, 17–90.

    Article  PubMed  CAS  Google Scholar 

  23. Auletta, F. J., Kelm, L. B., and Schofield, M. J. (1996). J. Reprod. Fertil. 103, 107–113.

    Google Scholar 

  24. Butler, W. R., Hotchkiss, J., and Knobil, E. (1975). Endocrinology 96, 1509–1512.

    PubMed  CAS  Google Scholar 

  25. Hackenberg, R., Hawighorst, T., Filmer, A., Nia, A. H., and Schulz, K. D. (1993). Breast Cancer Res. Treat. 25, 217–224.

    Article  PubMed  CAS  Google Scholar 

  26. Kemppainen, J. A., Lane, M. V., Sar, M., and Wilson, E. M. (1992). J. Biol. Chem. 267, 968–974.

    PubMed  CAS  Google Scholar 

  27. Yeh, S., Miyamoto, H., Shima, H., and Chang, C. (1998). Proc. Natl. Acad. Sci. USA 95, 5527–5532.

    Article  PubMed  CAS  Google Scholar 

  28. Hillier, S. G., Harlow, C. R., Shaw, H. J., Wickings, E. J., Dixson, A. F., and Hodges, J. K. (1987). In: The primate ovary. Stouffer, R. L. (ed.), Plenum, New York.

    Google Scholar 

  29. Zelinski-Wooten, M. B. and Stouffer, R. L. (1996). Trends Endocrinol. Metab. 7, 177–183.

    Article  CAS  PubMed  Google Scholar 

  30. Hibbert, M. L., Stouffer, R. L., Wolf, D. P., and Zelinski-Wooten, M. B. (1996). Proc. Natl. Acad. Sci. USA 93, 1897–1901.

    Article  PubMed  CAS  Google Scholar 

  31. Hillier, S. G., Tetsuka M., and Miro, F. (1996). In: Polycystic ovary syndrome. Chang, R. J., (ed.), Springer-Verlag, New York.

    Google Scholar 

  32. Bernhisel, M. A., Holman, J. F., Haney, A. F., and Schomberg, D. W. (1987). J. Clin. Endocrinol. Metab. 64, 1251–1256.

    PubMed  CAS  Google Scholar 

  33. Polan, M. L., Seu D., and Tarlatzis, B. (1986). J. Clin. Endocrinol. Metab. 62, 628–635.

    Article  PubMed  CAS  Google Scholar 

  34. Rodriguez-Rigau, L. J., Steinberger, E., Atkins, B. J., and Lucci, J. A. (1979). Fertil. Steril. 31, 448–450.

    PubMed  CAS  Google Scholar 

  35. Castracane, V. D., Wright, E., and Czar, P. L. (1983). Fertil. Steril. 40, 683–687.

    PubMed  CAS  Google Scholar 

  36. Sridaran, R., Smith, C. J., and Richards, J. S. (1991). Mol. Cell. Endocrinol. 77, 75–83.

    Article  PubMed  CAS  Google Scholar 

  37. Smith, K. D., Rodriguez-Rigau, L. J., Tcholakian, R. K., and Steinberger, E. (1979). Fertil. Steril. 32, 403–407.

    PubMed  CAS  Google Scholar 

  38. Coney, P. (1984). Fertil. Steril. 42, 667–682.

    PubMed  CAS  Google Scholar 

  39. Sridaran, R. and Gibori, G. (1981). Am. J. Physiol. 241, E444-E448.

    PubMed  CAS  Google Scholar 

  40. Molskness, T. A., Vande Voort, C. A., and Stouffer, R. L. (1987). Prostaglandins 34, 279–290.

    Article  PubMed  CAS  Google Scholar 

  41. Stouffer, R. L., Dahl, K. D., Hess, D. L., Woodruff, T. K., Mather, J. P., and Molskness, T. A. (1994). Biol. Reprod. 50, 888–895.

    Article  PubMed  CAS  Google Scholar 

  42. Resko, J. A., Ploem, J. G., and Stadelman, H. L. (1975). Endocrinology 97, 425–430.

    PubMed  CAS  Google Scholar 

  43. Vande Voort, C. A., Baughman, W. L., and Stouffer, R. L. (1989). J. In Vitro Fertil. Embryo Transfer 6, 85–91.

    Article  Google Scholar 

  44. Molskness, T. A., Zelinski-Wooten, M. B., Hild-Petito, S. A., and Stouffer, R. L. (1991). Biol. Reprod. 45, 273–281.

    Article  PubMed  CAS  Google Scholar 

  45. Ausubel, F. M. (ed.) (1987). Current protocols in molecular biology. Wiley and Sons: New York.

    Google Scholar 

  46. Chomczynski, P. and Sacchi, N. (1987). Anal. Biochem. 162, 156–159.

    Article  PubMed  CAS  Google Scholar 

  47. Chang, C., Kokontis, J., and Liao, S. (1988). Proc. Natl. Acad. Sci. USA 85, 7211–7215.

    Article  PubMed  CAS  Google Scholar 

  48. Resko, J. A., Ellinwood, W. E., Pasztor, L. M., and Huhl, A. E. (1980). J. Clin. Endocrinol. Metab. 50, 900–905.

    PubMed  CAS  Google Scholar 

  49. Hess, D. L., Spies, H. G., and Hendrickx, A. G. (1981). Biol. Reprod. 24, 609–616.

    Article  PubMed  CAS  Google Scholar 

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Author notes

  1. Kenneth R. Stott

    Present address: Gervais High School, 300 E. Douglas Avenue, 97026, Gervais, OR

  2. Salah E. Abdelgadir

    Present address: Department of Obstetrics and Gynecology, University of Nevada, University Institute of Fertility, 820 Shadow Lane, Suite 100, 89106, Las Vegas, NV

Authors and Affiliations

  1. Division of Reproductive Sciences, Oregon Regional Primate Research Center, 505 NW 185th Avenue, 97006, Beaverton, OR

    Diane M. Duffy, Kenneth R. Stott, Richard L. Stouffer & Mary B. Zelinski-Wooten

  2. Department of Physiology and Pharmacology, Oregon Health Sciences University, Portland, OR

    Salah E. Abdelgadir, John A. Resko & Richard L. Stouffer

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  1. Diane M. Duffy
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  2. Salah E. Abdelgadir
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Duffy, D.M., Abdelgadir, S.E., Stott, K.R. et al. Androgen receptor mRNA expression in the rhesus monkey ovary. Endocr 11, 23–30 (1999). https://doi.org/10.1385/ENDO:11:1:23

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  • DOI: https://doi.org/10.1385/ENDO:11:1:23

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