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TFAR19 gene changes the biophysical properties of murine erythroleukemia cells

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Abstract

TFAR19 is a novel apoptosis-related gene and can accelerate cell apoptosis in the presence of apoptosis inducements. Here, we studied the effects of TFAR19 on some biophysical properties of mouse erythroleukemia (MEL) cells and their molecular and structural basis. After transfected with TFAR19 and apoptosis, inducement, MEL revealed a high cell membrane fluidity, a decrease in resynthesis of phospholipids, an increase in the proteins/nucleic acids ratio, a relatively orderly cytoskeleton network, an impaired deformability, a low integrin aM expression, and a decrease in adhesion to endothelial cells. These findings suggest the potential of TFAR19 for antitumor cell migration, and thus for antitumor gene therapy.

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Authors and Affiliations

  1. Department of Biophysics, Health Science Center, Peking University, 100083, Beijing, P. R. China

    Li Gu, Yuhui Jiang, Weijuan Yao, Xiaofeng Xu & Zongyao Wen

  2. Department of Laboratory of Medical Immunology, Health Science Center, Peking University, 100083, Beijing, P. R. China

    Ying Wang

  3. Department of Medical Physics, Health Science Center, Peking University, 100083, Beijing, P. R. China

    Dagong Sun & Weibo Ka

  4. Department of Medical Mathematics, Health Science Center, Peking University, 100083, Beijing, P. R. China

    Zhiyu Tang

  5. Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California, San Diego, 92093-0412, La Jolla, CA, USA

    Shu Chien

Authors
  1. Li Gu
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  2. Yuhui Jiang
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  3. Ying Wang
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  8. Xiaofeng Xu
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  9. Zongyao Wen
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  10. Shu Chien
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Correspondence to Zongyao Wen.

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Contributed equally to this work.

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Gu, L., Jiang, Y., Wang, Y. et al. TFAR19 gene changes the biophysical properties of murine erythroleukemia cells. Cell Biochem Biophys 43, 355–363 (2005). https://doi.org/10.1385/CBB:43:3:355

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