Abstract
Actin and myosin interact in a cyclic series of steps linked to the hydrolysis of ATP that are representative of an ancient and widespread molecular mechanism. Spectroscopic findings are related to the analysis of the actin and myosin structures and results from kinetics, fibers, single molecules, electron microscopy, genetics, and a variety of other biophysical and biochemical studies on actin and myosin to provide an overview of the steps in this molecular process. The synthesis of the key findings from these fields reveals a highly efficient engine that amplifies subtle changes in the active site into unsurpassed molecular displacements. Recent developments in resonance energy-transfer spectroscopy and X-ray crystallography are enabling a detailed elucidation of the stages of a large power stroke that concurs with evidences from diverse lines of structural and kinetic inquiry. A complete image of actin and myosin motility appears to include twists, tilts, steps, and dynamics from both partners that could be described as a molecular dance.
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Vale, R. D. and Milligan, R. A. (2000) The way things move: looking under the hood of molecular motor proteins. Science 288, 88–95.
Kull, F. J., Sablin, E. P., Lau, R., Fletterick, R. J., and Vale, R. D. (1996) Crystal structure of the kinesin motor domain reveals a structural similarity to myosin. Nature 380, 550–555.
Kull, F. J., Vale, R. D., and Fletterick, R. J. (1998) The case for a common ancestor: kinesin and myosin motor proteins and G proteins. J. Muscle Res. Cell Motil. 19, 877–886.
Kinoshita, K., Sadanami, K., Kidera, A., and Go, N. (1999) Structural motif of phosphatebinding site common to various protein superfamilies: all-against-all structural comparison of protein-mononucleotide complexes. Protein Eng. 12, 11–14.
Sprang, S. R. (1997) G protein mechanisms: insights from structural analysis. Annu. Rev. Biochem. 66, 639–678.
Rees, D. C. and Howard, J. B. (2000) Nitrogenase: standing at the crossroads. Curr. Opin. Chem. Biol. 4, 559–566.
Patel, S. S. and Picha, K. M. (2000) Structure and function of hexameric helicases. Annu. Rev. Biochem. 69, 651–697.
Noji, H., Amano, T. and Yoshida, M. (1996) Molecular switch of F0F1-ATP synthase, G-protein, and other ATP-driven enzymes. J. Bioenerg. Biomembr. 28, 451–457.
Rayment, I., Smith, C., and Yount, R. G. (1996) The active site of myosin. Annu. Rev. Physiol. 58, 671–702.
Geeves, M. A. and Holmes, K. C. (1999) Structural mechanism of muscle contraction. Annu. Rev. Biochem. 68, 687–728.
Kabsch, W. and Holmes, K. C. (1995) The actin fold, FASEB J. 9, 167–174.
Flaherty, K. M., McKay, D. B., Kabsch, W., and Holmes, K. C. (1991) Similarity of the three-dimensional structures of actin and the ATPase fragment of a 70-kDa heat shock cognate protein. Proc. Natl. Acad. Sci. USA 88, 5041–5045.
van den Ent, F., Amos, L. A., and Löwe, J. (2001) Prokaryotic origin of the actin cytoskeleton. Nature 413, 39–44.
Löwe, J., Cordell S. C., and van den Ent, F. (2001) Crystal structure of the SMC head domain: an ABC ATPase with 900 residues antiparallel coiled-coil inserted. J. Mol. Biol. 306, 25–35.
Lockhart, A. and Kendrick-Jones, J. (1998) Nucleotide-dependent interaction of the N-terminal domain of MukB with microtubules. J. Struct. Biol. 124, 303–310.
Pollard, T. D. (2001) Genomics, the cytoskeleton and motility. Nature 409, 842–843.
Pantaloni, D., Le Clainche, C., and Carlier, M. F. (2001) Mechanism of actin-based motility. Science 292, 1502–1506.
Root, D. D. and Wang, K. (1994) Calmodulin-sensitive interaction of human nebulin fragments with actin and myosin. Biochemistry 33, 12,581–12,591.
Chalovich, J. M. (1992) Actin mediated regulation of muscle contraction. Pharmacol. Ther. 55, 95–148.
Sellers, J. R., Han, Y. J., and Kachar, B. (1991) The use of native thick filaments in in vitro motility assays. J. Cell Sci. 14(Suppl.), 67–71.
Sasaki, N., Asukagawa, H., Yasuda, R., Hiratsuka, T., and Sutoh, K. (1999) Deletion of the myopathy loop of Dictyostelium myosin II and its impact on motor functions. J. Biol. Chem. 274, 37,840–37,844.
Dalbev, R. E., Wells, J. A., and Yount, R. G. (1983) Trapping of transition metal-nucleotide complexes in myosin subfragment 1 by cross-linking thiols; divalent transition metal probes of the active site. Biochemistry 22, 490–496.
Reisler, E. (1982) Sulfhydryl modification and labeling of myosin. Methods Enzymol. 85, 84–93.
Joel, P. B., Trybus, K. M., and Sweeney, H. L. (2001) Two conserved lysines at the 50/20-kDa junction of myosin are necessary for triggering actin activation. J. Biol. Chem. 276, 2998–3003.
Sasaki, N., Ohkura, R., and Sutoh, K. (2000) Insertion or deletion of a single residue in the strut sequence of Dictyostelium myosin II abolishes strong binding to actin. J. Biol. Chem. 275, 38,705–38,709.
Grammer, J. C., Cremo, C. R., and Yount, R. G. (1988) UV-induced vanadate-dependent modification and cleavage of skeletal myosin subfragment 1 heavy chain. 1. Evidence for active site modification. Biochemistry 27, 8408–8415.
Xiao, M., Li, H., Snyder, G. E., Cooke, R., Yount, R. G., and Selvin, P. R. (1998) Conformational changes between the activesite and regulatory light chain of myosin as determined by luminescence resonance energy transfer: the effect of nucleotides and actin. Proc. Natl. Acad. Sci. USA 95, 15,309–15,314.
Bertrand, R., Derancourt, J., and Kassab, R. (2000) Fluorescence characterization of structural transitions at the strong actin binding motif in skeletal myosin affinity labeled at cysteine 540 with novel spectroscopic cysteaminyl mixed disulfides. Biochemistry 39, 14,626–14,637.
Bertrand, R., Derancourt, J., and Kassab, R. (1995) Production and properties of skeletal myosin subfragment 1 selectively labeled with fluorescein at lysine-553 proximal to the strong actin-binding site. Biochemistry 34, 9500–9507.
Lowey, S., Slayter, H. S., Weeds, A. G., and Baker, H. (1969) Substructure of the myosin molecule. I. Subfragments of myosin by enzymic degradation. J. Mol. Biol. 42, 1–29.
Murphy, C. T. and Spudich, J. A. (2000) Variable surface loops and myosin activity: accessories to a motor. J. Muscle Res. Cell Motil. 21, 139–151.
Ajtai, K., Peyser, Y. M., Park, S., Burghardt, T. P., and Muhlrad, A. (1999) Trinitrophenylated reactive lysine residue in myosin detects lever arm movement during the consecutive steps of ATP hydrolysis. Biochemistry 38, 6428–6440.
Ajtai, K., Garamszegi, S. P., Park, S., Velazquez Dones, A. L., and Burghardt, T. P. (2001) Structural characterization of beta-cardiac myosin subfragment 1 in solution, Biochemistry 40, 12,078–12,093.
Werber, M. M., Peyser, Y. M., and Muhlrad, A. (1987) Modification of myosin subfragment 1 tryptophans by dimenthyl (2-hydroxy-5-nitrobenzyl)sulfonium bromide. Biochemistry 26, 2903–2909.
Mornet, D. and Ue, K. (1985) Incorporation of 6-carboxyfluorescein into myosin subfragment 1. Biochemistry 24, 840–846.
Ho, G. and Chisholm, R. L. (1997) Substitution mutations in the myosin essential light chain lead to reduced actin-activated ATPase activity despite stoichiometric binding to the heavy chain. J. Biol. Chem. 272, 4522–4527.
Adhikari, B., Hideg, K., and Fajer, P. G. (1997) Independent mobility of catalytic and regulatory domains of myosin heads. Proc. Natl. Acad. Sci. USA 94, 9643–9647.
Sellers, J. R., Chock, P. B., and Adelstein, R. S. (1983) The apparently negatively cooperative phosphorylation of smooth muscle myosin at low ionic strength is related to its filamentous state. J. Biol. Chem. 258, 14,181–14,188.
Wolff-Long, V. L., Tao, T., and Lowey, S. (1995) Proximity relationships between engineered cysteine residues in chicken skeletal myosin regulatory light chain. A resonance energy transfer study. J. Biol. Chem. 270, 31,111–31,118.
Cook, R. K., Root, D. D., Miller, C., Reisler, E. and Rubinstein, P. A. (1993) Enhanced stimulation of myosin subfragment 1 ATPase activity by addition of negatively charged residues to the yeast actin N-terminus. J. Biol. Chem. 268, 2410–2415.
Razzaq, A., Schmitz, S., Veigel, C., Molloy, J. E., Geeves, M. A., and Sparrow, J. C. (1999) Actin residue glu(93) is identified as an amino acid affecting myosin binding. J. Biol. Chem. 274, 28,321–28,328.
Schwyter, D., Phillips, M., and Reisler, E. (1989) Subtilisin-cleaved actin: polymerization and interaction with myosin subfragment 1. Biochemistry 28, 5889–5895.
Eli-Berchoer, L., Hegyi, G., Patthy, A., Reisler, E., and Muhlrad, A. (2000) Effect of intramolecular cross-linking between glutamine-41 and lysine-50 on actin structure and function. J. Muscle Res. Cell Motil. 21, 405–414.
Takashi, R. (1988) A novel actin label: a fluorescent probe at glutamin-41, and its consequences. Biochemistry 27, 938–943.
Miller, L., Phillips, M., and Reisler, E. (1988) Polymerization of actin modified with fluorescein isothiocyanate. Eur. J. Biochem. 174, 23–29.
Duke, J., Takashi, R., Use, K., and Morales, M. F. (1976) Reciprocal reactivities of specific thiols when actin binds to myosin. Proc. Natl. Acad. Sci. USA 73, 302–306.
Hozumi, T., Miki, M., and Higashi-Fujime, S. (1996) Maleimidobenzoyl actin: its biochemical properties and in vitro motility. J. Biochem. 119, 151–156.
Crosbie, R. H., Miller, C., Cheung, P., Goodnight, T., Muhlrad, A., and Reisler, E. (1994) Structural connectivity in actin: effect of C-terminal modifications on the properties of actin. Biophys. J. 67, 1957–1964.
O'Donoghue, S. I., Miki, M., and dos Remedios, C. G. (1992) Removing the two C-terminal residues of actin affects the filament structure. Arch. Biochem. Biophys. 293, 110–116.
El-Saleh, S. C., Thieret, R., Johnson, P., and Potter, J. D. (1984) Modification of Lys-237 on actin by 2,4-pentanedione. Alteration of the interaction of actin with tropomyosin. J. Biol. Chem. 259, 11,014–11,021.
Terashima, M., Yamamori, C., and Shimoyama, M. (1995) ADP-ribosylation of Arg28 and Arg206 on the actin molecule by chicken arginine-specific ADP-ribosyltransferase. Eur. J. Biochem. 231, 242–249.
Chantler, P. D. and Gratzer, W. B. (1975) Effects of specific chemical modification of actin. Eur. J. Biochem. 60, 67–72.
Prochniewicz, E., Katayama, E., Yanagida, T., and Thomas, D. D. (1993) Cooperativity in Factin: chemical modifications of actin monomers affect the functional interactions of myosin with unmodified monomers in the same actin filament. Biophys. J. 65, 113–123.
Sutoh, K. (1982) Identification of myosin-binding sites on the actin sequence. Biochemistry 21, 3654–3661.
Bonafé, N., Chaussepied, P., Capony, J. P., Derancourt, J., and Kassab, R. (1993) Photochemical cross-linking of the skeletal myosin head heavy chain to actin subdomain-1 at Arg95 and Arg28. Eur. J. Biochem. 213, 1243–1254.
Eligula, L., Chuang, L., Phillips, M. L., Motoki, M., Seguro, K., and Muhlrad, A. (1998) Transglutaminase-induced cross-linking between subdomain 2 of G-actin and the 636–642 lysine-rich loop of myosin subfragment 1. Biophys. J. 74, 953–963.
Lymn, R. W. and Taylor, E. W. (1971) Mechanism of adenosine triphosphate hydrolysis by actomyosin. Biochemistry 10, 4617–4624.
Bagshaw, C. R. and Trentham, D. R. (1975) Transient kinetic and isotopic tracer studies of themyosin adenosine triphosphatase reaction. J. Supramol. Struct. 3, 315–322.
Bagshaw, C. R., Trentham, D. R., Wolcott, R. G., and Boyer, P. D. (1975) Oxygen exchange in the gamma-phosphoryl group of protein-bound ATP during Mg2+-dependent adenosine triphosphatase activity of myosin. Proc. Natl. Acad. Sci. USA 72, 2592–2596.
Dale, M. P. and Hackney, D. D. (1987) Analysis of positional isotope exchange in ATP by cleavage of the beta P-O gamma P bond. Demonstration of negligible positional isotope exchange by myosin. Biochemistry 26, 8365–8372.
Evans, J. A. and Eisenberg, E. (1989) Intermediate oxygen exchange catalyzed by the actin-activated skeletal myosin adenosinetriphosphatase. Biochemistry 28, 7741–7747.
Tikunov, B. A., Sweeney, H. L., and Rome, L. C. (2001) Quantitative electrophoretic analysis of myosin heavy chains in single muscle fibers. J. Appl. Physiol. 90, 1927–1935.
Taylor, E. W. (1991) Kinetic studies on the association and dissociation of myosin subfragment 1 and actin. J. Biol. Chem. 266, 294–302.
Dantzig, J. A., Barsotti, R. J., Manz, S., Sweeney, H. L., and Goldman, Y. E. (1999) The ADP release step of the smooth muscle crossbridge cycle is not directly associated with force generation. Biophys. J. 77, 386–397.
Geeves, M. A. and Conibear, P. B. (1995) The role of three-state docking of myosin S1 with actin in force generation. Biophys. J. 68, 194S-199S.
Gordon, A. M., Homsher, E., and Regnier, M. (2000) Regulation of contraction in striated muscle. Physiol. Rev. 80, 853–824.
Friedman, A. L., Geeves, M. A., Manstein, D. J. and Spudich, J. A. (1998) Kinetic characterization of myosin head fragments with long-lived myosin ATP states. Biochemistry 37, 9679–9687.
Konrad, M. and Goody, R. S. (1982) Kinetic and thermodynamic properties of the ternary complex between F-actin, myosin subfragment 1 and adenosine 5′-[beta, gamma-imido]triphosphate. Eur. J. Biochem. 128, 547–555.
Lovell, S. J. and Harrington, W. F. (1981) Measurement of the fraction of myosin heads bound to actin in rabbit skeletal myofibrils in rigor. J. Mol. Biol. 149, 659–674.
Goodno, C. C. and Taylor, E. W. (1982) Inhibition of actomyosin ATPase by vanadate. Proc. Natl. Acad. Sci. USA 79, 21–25.
Phan, B. C., Faller, L. D., and Reisler, E. (1993) Kinetic and equilibrium analysis of the interactions of actomyosin subfragment-1.ADP with beryllium fluoride. Biochemistry 32, 7712–7719.
Maruta, S., Henry, G. D., Sykes, B. D., and Ikebe, M. (1993) Formation of the stable myosin-ADP-aluminum fluoride and myosin-ADP-beryllium fluoride complexes and their analysis using 19F NMR. J. Biol. Chem. 268, 7093–7100.
Werber, M. M., Peyser, Y. M., and Muhlard, A. (1992) Characterization of stable beryllium fluoride, aluminum fluoride, and vanadate containing myosin subfragment 1-nucleotide complexes. Biochemistry 31, 7190–7197.
Peyser, Y. M., Ajtai, K., Werber, M. M., Burghardt, T. P., and Muhlrad, A. (1997) Effect of metal cations on the conformation of myosin subfragment-1-ADP-phosphate analog complexes: a near-UV circular dichroism study. Biochemistry 36, 5170–5178.
Chase, P. B., Martyn, D. A., and Hannon, J. D., (1994) Activation dependence and kinetics of force and stiffness inhibition by aluminiofluoride, a slowly dissociating analogue of inorganic phosphate, in chemically skinned fibres from rabbit psoas muscle. J. Muscle Res. Cell Motil. 15, 119–129.
Fisher, A. J., Smith, C. A., Thoden, J. B., Smith, R., Sutoh, K., Holden, H. M., et al. (1995) X-ray structures of the myosin motor domain of Dictyostelium discoideum complexed with MgADP·BeF x and MgADP·AlF4 −. Biochemistry 34, 8960–8972.
Peyser, Y. M., Ajtai, K., Burghardt, T. P., and Muhlrad, A. (2001) Effect of ionic strength on the conformation of myosin subfragment 1-nucleotide complexes. Biophys. J. 81, 1101–1114.
Rayment, I., Rypniewski, W. R., Schmidt-Bäse, K., Smith, R., Tomchick, D. R., Benning, M. M., et al. (1993) Three-dimensional structure of myosin subfragment-1: a molecular motor. Science 261, 50–58.
Houdusse, A., Szent-Györgyi, A. G., and Cohen, C. (2000) Three conformational states of scallop myosin S1. Proc. Natl. Acad. Sci. USA 97, 11,238–11,243.
Bauer, C. B., Holden, H. M., Thoden, J. B., Smith, R., and Rayment, I. (2000) X-ray structures of the apo and MgATP-bound states of Dictyostelium discoideum myosin motor domain. J. Biol. Chem. 275, 38,494–38,499.
Gulick, A. M., Bauer, C. B., Thoden, J. B., Pate, E., Yount, R. G., and Rayment, I. (2000) X-ray structures of the Dictyostelium discoideum myosin motor domain with six non-nucleotide analogs. J. Biol. Chem. 275, 398–408.
Bauer, C. B., Kuhlman, P. A., Bagshaw, C. R., and Rayment, I. (1997) X-ray crystal structure and solution fluorescence characterization of Mg. 2′(3′)-O-(N-methylanthraniloyl) nucleotides bound to the Dictyostelium discoideum myosin motor domain. J. Mol. Biol. 274, 394–407.
Root, D. D., Stewart, S., and Xu, J. (2002) Dynamic docking of myosin and actin observed with resonance energy transfer. Biochemistry 41, 1786–1794.
Shih, W. M., Gryczynski, Z., Lakowicz,J. R., and Spudich, J. A. (2000) A FRET-based sensor reveals large ATP hydrolysis-induced conformational changes and three distinct states of the molecular motor myosin. Cell 102, 683–694.
Rosenfeld, S. S. and Taylor, E. W. (1984) Reactions of 1-N6-ethenoadenosine nucleotides with myosin subfragment 1 and acto-subfragment 1 of skeletal and smooth muscle. J. Biol. Chem. 259, 11,920–11,929.
Hill, T. L., Eisenberg, E., and Greene,L. (1980) Theoretical model for the cooperative equilibrium binding of myosin subfragment 1 to the actin-troponin-tropomyosin complex. Proc. Natl. Acad. Sci. USA 77, 3186–3190.
Stein, L. A. (1995–96) Modeling of the actomyosin ATPase activity. Origin of the initial phosphate burst and implications of the phosphate release kinetics. Cell Biochem. Biophys. 27, 63–96.
Sleep, J. A. and Hutton, R. L. (1980) Exchange between inorganic phosphate and adenosine 5′-triphosphate in the medium by actomyosin subfragment 1. Biochemistry 19, 1276–1283.
Houdusse, A. and Sweeney, H. L. (2001) Myosin motors: missing structures and hidden springs. Curr. Opin. Struct. Biol. 11, 182–194.
Veigel, C., Coluccio, L. M., Jontes, J. D., Sparrow, J. C., Milligan, R. A., and Molloy, J. E. (1999) The motor protein myosin-I produces its working stroke in two steps. Nature 398, 530–533.
Kitamura, K., Tokunaga, M., Iwane, A. H., and Yanagida, T. (1999) A single myosin head moves along an actin filament with regular steps of 5.3 nanometres. Nature 397, 129–134.
Hill, A. V. (1964) The effect of load on the heat of shortening of muscle. Proc. R. Soc. Lond. B Biol. Sci. 159, 297–318.
Homsher, E. (1987) Muscle enthalpy production and its relationship to actomyosin ATPase. Annu. Rev. Physiol. 49, 673–690.
Root, D. D. and Reisler, E. (1992) Cooperativity of thiol-modified myosin filaments: ATPase and motility assays of myosin function. Biophys. J. 63, 730–740.
He, Z.-H., Bottinelli, R., Pellegrino, M. A., Ferenczi, M. A., and Reggiani, C. (2000) ATP consumption and efficiency of human single muscle fibers with different myosin isoform composition. Biophys. J. 79, 945–961.
Fenn, W. O. 91923) A quantitative comparison between the energy liberated and the work performed by the isolated sartorius muscle of the frog. J. Physiol. (Lond.) 58, 175–203.
Xu, J. and Root, D. D. (2000) Conformational selection during weak binding at the actin and myosin interface. Biophys. J. 79, 1498–1510.
Altringham, I. D., Yancey, P. H., and Johnston, I. A. (1980) Limitations in the use of actomyosin threads as model contractile systems. Nature 287, 338–340.
Sheetz, M. P. and Spudich, J. A. (1983) Movement of myosin-coated fluorescent beads on actin cables in vitro Nature 303, 31–35.
Yanagida, T., Nakase, M., Nishiyama, K., and Oosawa, F. (1984) Direct observation of motion of single F-actin filaments in the presence of myosin. Nature 307, 58–60.
Kron, S. J. and Spudich, J. A. (1986) Fluorescent actin filaments move on myosin fixed to a glass surface. Proc. Natl. Acad. Sci. USA 83, 6272–6276.
Homsher, E., Wang, F., and Sellers, J. R. (1992) Factors affecting movement of F-actin filaments propelled by skeletal muscle heavy meromyosin. Am. J. Physiol. 262, C714-C723.
Kawai, M., Kawaguchi, K., Saito, M., and Ishiwata, S. (2000) Temperature change does not affect force between single actin filaments and HMM from rabbit muscles. Biophys J. 78, 3112–3119.
Davis, J. S. (1998) Force generation simplified. Insights from laser temperature-jump experiments on contracting muscle fibers. Adv. Exp. Med. Biol. 453, 343–351.
Finer, J. T., Mehta, A. D., and Spudich, J. A. (1995) Characterization of single actin-myosin interactions. Biophys. J. 68, 291S-296S.
Warshaw, D. M., Desrosiers, J. M., Work, S. S., and Trybus, K. M. (1991) Effects of MgATP, MgADP, and Pi on actin movement by smooth muscle myosin. J. Biol. Chem. 266, 24,339–24,343.
Wada, H., Yamada, T., and Sugi, H. (1996) Effect of inorganic phosphate and ADP on the myofilament sliding induced by laser flash photolysis of caged ATP. Biochimi. Biophys. Acta 1274, 89–93.
Bing, W., Knott, A., and Marston, S. B. (2000) A simple method for measuring the relative force exerted by myosin on actin filaments in the in vitro motility assay: evidence that tropomyosin and troponin increase force in single thin filaments. Biochem. J 350, 693–699.
Pate, E. and Cooke, R. (1989) Addition of phosphate to active muscle fibers probes actomyosin states within the powerstroke. Pflugers Arch. 414, 73–81.
Kato, H., Nishizaka, T., Iga, T., Kinosita, K., Jr., and Ishiwata, S. (1999) Imaging of thermal activation of actomyosin motors. Proc. Natl. Acad. Sci. USA 96, 9602–9606.
Ishijima, A., Kojima, H., Higuchi, H., and Harada, Y. (1996) Multiple- and single-molecule analysis of the actomyosin motor by nanometer-piconewton manipulation with a microneedle: unitary steps and forces. Biophys. J. 70, 383–400.
Veigel, C., Bartoo, M. L., White, D. C, Sparrow, J. C., and Molloy, J. E. (1998) The stiffness of rabbit skeletal actomyosin crossbridges determined with an optical tweezers transducer. Biophys. J. 75, 1424–1438.
Tyska, M. J., Dupuis, D. E., Guilford, W. H., Patlak, J. B., Waller, G. S., Trybus, K. M., et al. (1999) Two heads of myosin are better than one for generating force and motion. Proc. Natl. Acad. Sci. USA 96, 4402–4407.
Sugiura, S., Kobayakawa, N., Fujita, H., Yamashita, H., Momomura, S., Chaen, S., et al. (1998) Comparison of unitary displacements and forces between 2 cardiac myosin isoforms by the optical trap technique: molecular basis for cardiac adaptation. Circ. Res. 82, 1029–1034.
Miyata, H., Yoshikawa, H., Hakozaki, H., Suzuki, N., Furuno, T., Ikegami, A., et al. (1995) Mechanical measurements of single actomyosin motor force. Biophys. J. 68, 286S-289S.
Merah, Z. and Morel, J. E. (1993) Isometric tension exerted by a myofibril of the frog at 0 degree C: geometrical considerations. J. Muscle Res. Cell Motil. 14, 552–553.
Nishizaka, T., Miyata, H., Yoshikawa, H., Ishiwata, S., and Kinosita, K. (1995) Unbinding force of a single motor molecule of muscle measured using optical tweezers. Nature 377, 251–254.
Nakajima, H., Kunioka, Y., Nakano, K., Shimizu, K., Seto, M., and Ando, T. (1997) Scanning force microscopy of the interaction events between a single molecule of heavy meromyosin and actin. Biochem. Biophys. Res. Commun. 234, 178–182.
Huxley A. F. and Simmons, R. M. (1971) Proposed mechanism of force generation in striated muscle. Nature 233, 533–538.
Oplatka, A. (2000) A new outlook on the energetics of muscle contraction. Biophys. Chem. 86, 49–57.
Woledge, R. C., Curtin, N. A., and Homsher, E. (1985) Energetic aspects of muscle contraction. Monogr. Physiol. Soc. 41, 1–357.
Sugi, H., Iwamoto, H., Akimoto, T., and Ushitani, H. (1998) Load-dependent mechanical efficiency of individual myosin heads in skeletal muscle fibers activated by laser flash photolysis of caged calcium in the presence of a limited amount of ATP. Adv. Exp. Med. Biol. 453, 557–566.
Blange, T., van der Heide, U. A., Treijtel, B. W., and de Beer, E. L. (1997) The effect of actin filament compliance on the interpretation of the elastic properties of skeletal muscle fibres. J. Muscle Res. Cell Motil. 18, 125–131.
Wakabayashi, K., Sugimoto, Y., Tanaka, H., Ueno, Y., Takezawa, Y., and Amemiya, Y., (1994) X-ray diffraction evidence for the extensibility of actin and myosin filaments during muscle contraction. Biophys. J. 67, 2422–2435.
Kojima, H., Ishijima, A., and Yanagida, T. (1994) Direct measurement of stiffness of single actin filaments with and without tropomyosin by in vitro nanomanipulation. Proc. Natl. Acad. Sci. USA 91, 12,962–12,966.
Huxley, H. E. and Hansen, J. (1954) Changes in the cross-striations of muscle during contraction and stretch and their structural interpretation. Nature 173, 193–196.
Huxley, A. F. and Niedergerke R. C. (1954) Structural changes in muscle during contraction. Nature 173, 191–193.
Reedy, M. K., Holmes K. C., and Tregear, R. T. (1965) Induced changes in orientation of the cross-bridges of glycerinated insect flight muscle. Nature 207, 1276–1280.
Jontes, J. D., Ostap, E. M., Pollard, T. D., and Milligan, R. A. (1998) Three-dimensional structure of Acanthamoeba castellanii myosin-IB (MIB) determined by cryoelectron microscopy of decorated actin filaments. J. Cell Biol. 141, 155–162.
Rayment, I., Holden, H. M., Whittaker M., Yohn, C. B., Lorenz, M., Holmes, K. C., et al. (1993) Structure of the actin-myosin complex and its implications for muscle contraction. Science 261, 58–65.
Flicker, P. F., Milligan, R. A., and Applegate, D. (1991) Cryo-electron microscopy of S1-decorated actin filaments. Adv. Biophys. 27, 185–196.
Whittaker, M., Wilson-Kubalek, E. M. Smith, J. E., Faust, L., Milligan, R. A., and Sweeney, H. L. (1995) A 35-A movement of smooth muscle myosin on ADP release. Nature 378, 748–751.
Jontes, J. D., Wilson-Kubalek, E. M., and Milligan, R. A. (1995) A 32 degree tail swing in brush border myosinI on ADP release. Nature 378, 751–753.
Carragher, B. O., Cheng, N., Wang, Z. Y., Korn, E. D., Reilein, A., Belnap, D. M., et al. (1998) Structural invariance of constitutively active and inactive mutants of Acanthamoeba myosinIC bound to F-actin in the rigor and ADP-bound states. Proc. Natl. Acad. Sci. USA 95, 15,206–15,211.
Khromov, A. S., Somlyo, A. P., and Somlyo, A. V. (2001) Photolytic release of MgADP reduces rigor force in smooth muscle. Biophys. J. 80, 1905–1914.
Schroder, R. R., Manstein, D. J., Jahn, W., Holden, H., Rayment, I., Holmes, K. C., et al. (1993) Three-dimensional atomic model of F-actin decorated with Dictyostelium myosin S1. Nature 364, 171–174.
Volkmann, N., Hanein, D., Ouyang, G., Trybus, K. M., DeRosier, D. J., and Lowey, S. (2000) Evidence for cleft closure in actomyosin upon ADP release. Nat. Struct. Biol. 7, 1147–1155.
Applegate, D. and Flicker, P. (1987) New states of actomyosin. J. Biol. Chem. 262, 6856–6863.
Pollard, T. D., Bhandari, D., Maupin, P., Wachsstock, D., Weeds, A. G., and Zot, H. G. (1993) Direct visualization by electron microscopy of the weakly bound intermediates in the actomyosin adenosine triphosphatase cycle. Biophys. J. 64, 454–471.
Walker, M. L., Burgess, S. A., Sellers, J. R., Wang, F., Hammer, J. A., 3rd, Trinick, J., et al. (2000) Two-headed binding of a processive myosin to F-actin. Nature 405, 804–807.
Hallett, P., Offer, G., and Miles, M. J. (1995) Atomic force microscopy of the myosin molecule. Biophys. J. 68, 1604–1606.
Zhang, Y., Shao, Z., Somlyo, A. P., and Somlyo, A. V. (1997) Cryo-atomic force microscopy of smooth muscle myosin. Biophys. J. 72, 1308–1318.
Shao, Z., Shi, D., and Somlyo, A. V. (2000) Cryoatomic force microscopy of filamentous actin. Biophys. J. 78, 950–958.
Levitsky, D. I., Nikoleeva, O. P., Orlov, V. N., Pavlov, D. A., Ponomarev, M. A., and Rostkova, E. V. (1998) Differential scanning calorimetric studies on myosin and actin. Biochemistry (Moscow), 63, 322–333.
Kaspieva, O. V., Nikolaeva, O. P., Orlov, V. N., Ponomarev, M. A., Drachev, V. A., and Levitsky, D. I. (2001) Changes in the thermal unfolding of p-phenylenedimaleimide-modified myosin subfragment 1 induced by its ‘weak’ binding to F-actin. FEBS Lett. 489, 144–148.
Keller, T. C., 3rd and Mooseker, M. S. (1982) Ca++-calmodulin-dependent phosphorylation of myosin, and its role in brush border contraction in vitro. J. Cell Biol. 95, 943–959.
Trybus, K. M., Huiatt, T. W., and Lowey, S. (1982) A bent monomeric conformation of myosin from smooth muscle. Proc. Natl. Acad. Sci. USA 79, 6151–6155.
Wendt, T., Taylor, D., Trybus, K. M., and Taylor, K. (2001) Three-dimensional image reconstruction of dephosphorylated smooth muscle heavy meromyosin reveals asymmetry in the interaction between myosin heads and placement of subfragment 2. Proc. Natl. Acad. Sci. USA 98, 4361–4366.
Espreafico, E. M., Cheney, R. E., Matteoli, M., Nascimento, A. A., De Camilli, P. V., Larson, R. E., et al. (1992) Primary structure and cellular localization of chicken brain myosin-V (p190), an unconventional myosin with calmodulin light chains. J. Cell Biol. 119, 1541–1557.
Kouyama, T. and Mihashi, K. (1981) Fluorimetry study of N-(1-pyrenyl)iodoacetamide-labelled F-actin. Local structural change of actin protomer both on polymerization and on binding of heavy meromyosin. Eur. J. Biochem. 114, 33–38.
Andreev, O. A., Saraswat, L. D., Lowey, S., Slaughter, C., and Borejdo, J. (1999) Interaction of the N-terminus of chicken skeletal essential light chain 1 with F-actin. Biochemistry 38, 2480–2485.
Kabsch, W., Mannherz, H. G., Suck, D., Pai, E. F., and Holmes, K. C. (1990) Atomic structure of the actin: DNase I complex. Nature 347, 37–44.
Holmes, K. C., Popp, D., Gebhard, W., and Kabsch, W. (1990) Atomic model of the actin filament. Nature 347, 44–49.
Lorenz, M., Popp, D., and Holmes, K. C. (1993) Refinement of the F-actin model against X-ray fiber diffraction data by the use of a directed mutation algorithm. J. Mol. Biol. 234, 826–836.
Bremer, A., Henn, C., Goldie, K. N., Engel, A., Smith, P. R., and Aebi, U. (1994) Towards atomic interpretation of F-actin filament threedimensional reconstructions. J. Mol. Biol. 242, 683–700.
Taylor, K. A., Schmitz, H., Reedy, M. C., Goldman, Y. E., Franzini-Armstrong, C., Sasaki, H., et al. (1999) Tomographic 3D reconstruction of quick-frozen, Ca2+-activated contracting insect flight muscle. Cell 99, 421–431.
Mendelson, R. and Morris, E. P. (1997) The structure of the acto-myosin subfragment 1 complex: results of searches using data from electron microscopy and x-ray crystallography. Proc. Natl. Acad. Sci. USA 94, 8533–8538.
Shimada, T., Sasaki, N., Ohkura, R., and Sutoh, K. (1997) Alanine scanning mutagenesis of the switch I region in the ATPase site of Dictyostelium discoideum myosin II. Biochemistry 36, 14,037–14,043.
Sasaki, N., Shimada, T., and Sutoh, K. (1998) Mutational analysis of the switch II loop of Dictyostelium myosin II. J. Biol. Chem. 273, 20,334–20,340.
Shih, W. M. and Spudich, J. A. (2001) The myosin relay helix to converter interface remains intact throughout the actomyosin ATPase cycle. J. Biol. Chem. 276, 19,491–19,494.
Yengo, C. M., Chrin, L. R., Rovner, A. S., and Berger, C. L. (2000) Tryptophan 512 is sensitive to conformational changes in the rigid relay loop of smooth muscle myosin during the MgATPase cycle. J. Biol. Chem. 275, 25,481–25,487.
Schutt, C. E., Myslik, J. C., Rozycki, M. D., Goonesekere, N. C., and Lindberg, U. (1993) The structure of crystalline profilin-beta-actin. Nature 365, 810–816.
McLaughlin, P. J., Gooch, J. T., Mannherz, H. G., and Weeds, A. G. (1993) Structure of gelsolin segment 1-actin complex and the mechanism of filament severing. Nature 364, 685–692.
Otterbein, L. R., Graceffa, P., and Dominguez R. (2001) The crystal structure of uncomplexed actin in the ADP state. Science 293, 708–711.
Robinson, R. C., Mejillano, M., Le, V. P., Burtnick, L. D., Yin, H. L., Choe, S. (1999) Domain mvoement in gelsolin: a calcium-activated switch. Science 286, 1939–1942.
Dominguez, R., Freyzon, Y., Trybus, K. M., and Cohen, C. (1998) Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state. Cell 94, 559–571.
Houdusse, A., Kalabokis, V. N., Himmel, D., Szent-Györgyi, A. G., and Cohen, C. (1999) Atomic structure of scallop myosin subfragment S1 complexed with MgADP: a novel conformation of the myosin head. Cell 97, 459–470.
Smith, C. A. and Rayment, I. (1996) X-ray structure of the magnesium (II)·ADP· vanadate complex of the Dictyostelium discoideum myosin motor domain to 1.9 Å resolution. Biochemistry 35, 5404–5417.
Yount, R. G., Lawson, D., and Rayment, I. (1995) Is myosin a “back door” enzyme? Biophys J. 68, 44S-47S.
Minehardt, T. J., Cooke, R., Pate, E., and Kollman, P. A. (2001) Molecular dynamics study of the energetic, mechanistic, and structural implications of a closed phosphate tube in ncd. Biophys. J. 80, 1151–1168.
Nyitrai, M., Hild, G., Bódis, E., Lukács, A., and Somogyi, B. (2000) Flexibility of myosin-subfragment-1 in its complex with actin as revealed by fluorescence resonance energy transfer. Eur. J. Biochem. 267, 4334–4338.
Baker, J. E., Brust-Mascher, I., Ramachandran, S., LaConte, L. E., and Thomas, D. D. (1998) A large and distinct rotation of the myosin light chain domain occurs upon muscle contraction. Proc. Natl. Acad. Sci. USA 95, 2944–2949.
Brown, L. J., Klonis, N., Sawyer, W. H., Fajer, P. G., and Hambly, B. D. (2001) Independent movement of the regulatory and catalytic domains of myosin heads revealed by phosphorescence anisotropy. Biochemistry 40, 8283–8291.
Burghardt, T. P., Cruz-Walker, A. R., Park, S., and Ajtai, K. (2001) Conformation of myosin interdomain interactions during contraction: deductions from muscle fibers using polarized fluorescence. Biochemistry 40, 4821–4833.
Andreev, O. A., Andreeva, A. L., Markin, V. S., and Borejdo, J. (1993) Two different rigor complexes of myosin subfragment 1 and actin. Biochemistry 32, 12,046–12,053.
Thomas, D. D., Ramachandran, S., Roopanrine, O., Hayden, D. W., and Ostap, E. M. (1995) The mechanism of force generation in myosin: a disorder-to-order transition, coupled to internal structural changes. Biophys. J. 68, 135S-141S.
Homma, K., Yoshimura, M., Saito, J., Ikebe, R., and Ikebe, M. (2001) The core of the motor domain determines the direction of myosin movement. Nature 412, 831–834.
Cooper, W. C., Chrin, L. R., and Berger, C. L. (2000) Detection of fluorescently labeled actinbound cross-bridges in actively contracting myofibrils. Biophys. J. 78, 1449–1457.
Root, D. D. and Reisler, E. (1992) The accessibility of etheno-nucleotides to collisional quenchers and the nucleotide cleft in G- and F-actin. Protein Sci. 1, 1014–1022.
Yamamoto, T., Nakayama, S., Kobayashi, N., Munekata, E., and Ando, T. (1994) Determination of electrostatic potetial around specific locations on the surface of actin by diffusion-enhanced fluorescence resonance energy transfer. J. Mol. Biol. 241, 714–731.
Corrie, J. E., Brandmeier, B. D., Ferguson, R. E., Trentham, D. R., Kendrick-Jones, J., Hopkins, S. C., et al. (1999) Dynamic measurement of myosin light-chain-domain tilt and twist in muscle contraction. Nature 400, 425–430.
Highsmith, S. and Jardetzky, O. (1983) Actininduced changes in the dynamics of myosin subfragment-1 detected by nuclear magnetic resonance. Ciba Found. Symp. 93, 156–158.
Barnett, V. A. and Thomas, D. D. (1984) Saturation transfer electron paramagnetic resonance of spin-labeled muscle fibers. Dependence of myosin head rotational motion on sarcomere length. J. Mol. Biol. 179, 83–102.
Kim, E., Miller, C. J., Motoki, M., Seguro, K., Muhlrad, A., and Reisler, E. (1996) Myosininduced changes in F-actin: fluorescence prob ing of subdomain 2 by dansyl ethylenediamine attached to Gln-41. Biophys. J. 70, 1439–1446.
Borovikov, Y. S., Moraczewska, J., Khoroshev, M. I., and Strzelecka-Goaszewska, H. (2000) Proteolytic cleavage of actin within the DNase-I-binding loop changes the conformation of Factin and its sensitivity to myosin binding. Biochim. Biophys. Acta 1478, 138–151.
Irving, M., Allen, T. S., Sabido-David, C., Craik, J. S., Brandmeier, B., Kendrick-Jones, J., et al. (1995) Tilting of the light-chain region of myosin during step length changes and active force generation in skeletal muscle. Nature 375, 688–691.
Sabido-David, C., Hopkins, S. C., Saraswat, L. D., Lowey, S., Goldman, Y. E., and Irving, M. (1998) Orientation changes of fluorescent probes at five sites on the myosin regulatory light chain during contraction of single skeletal muscle fibres. J. Mol. Biol. 279, 387–402.
Haugland, R. P. (1975) Myosin structure. Proximity measurements by fluorescence energy transfer. J. Supramol. Struct. 3, 338–347.
dos Remedios, C. G. and Moens, P. D. (1995) Actin and the actomyosin interface: a review. Biochim. Biophys. Acta 1228, 99–124.
Förster, T. (1948) Zwischenmolekulare Energiwanderung und Fluoreszence [Intermolecular energy migration and fluorescence]. Ann. Phys. 2, 55–75.
dos Remedios, C. G. and Moens, P. D. (1995) Fluorescence resonance energy transfer spectroscopy is a reliable “ruler” for measuring structural changes in proteins. Dispelling the problem of the unknown orientation factor. J. Struct. Biol. 115, 175–185.
Cantor, C. R. and Schimmel, P. R. (1980) Biophysical Chemistry: II. Techniques for the Study of Biological Structure and Function, W. H. Freeman, New York.
Schiller, P. W. (1975) The measurement of intramolecular distances by energy transfer, in Biochemical Fluorescence: Concepts (Chen, R. F. and Edelhoch, H., eds.), Marcel Dekker, New York, pp. 285–303.
Selvin, P. R. (1995) Fluorescence resonance energy transfer. Meth. Enzymol. 246, 300–334.
Stryer, L. and Haugland, R. P. (1967) Energy transfer: a spectroscopic ruler. Proc. Natl. Acad. Sci. USA 58, 719–726.
Root, D. D., Shangguan, X., Xu, J., and McAllister, M. (1999) Determination of fluorescent probe orientations on biomolecules by conformational searching: algorithm testing and applications to the atomic model of myosin. J. Struct. Biol. 127, 22–34.
dos Remedios, C. G., Miki, M., and Barden, J. A. (1987) Fluorescence resonance energy transfer measurements of distances in actin and myosin. A critical evaluation. J. Muscle Res. Cell Motil. 8, 97–117.
O'Donoghue, S. I., Hambly, B. D., and dos Remedios, C. G. (1992) Models of the actin monomer and filament from fluorescence resonance-energy transfer. Eur. J. Biochem. 205, 591–601.
Xu, J. and Root, D. D. (1998) Domain motion between the regulatory light chain and the nucleotide site in skeletal myosin. J. Struct. Biol. 123, 150–161.
Smyczynski, C. and Kasprzak, A. A. (1997) Effect of nucleotides and actin on the orientation of the light chain-binding domain in myosin subfragment 1. Biochemistry 36, 13,201–13,207.
Yengo, C. M., Chrin, L. R., and Berger, C. L. (2000) Interaction of myosin LYS-553 with the C-terminus and DNase I-binding loop of actin examined by fluorescence resonance energy transfer. J. Struct. Biol. 131, 187–196.
Chakrabarty, T., Xiao, M., Cooke, R., and Selvin, P. R. (2002) Holding two heads together: stability of the myosin II rod measured by resonance energy transfer between the heads. Proc. Natl. Acad. Sci. USA 99, 6011–6016.
Maruta, S. and Homma, K. (2000) Conformational changes in the unique loops bordering the ATP binding cleft of skeletal muscle myosin mediate energy transduction. J. Biochem. 128, 695–704.
Palm, T., Sale, K., Brown, L., Li, H., Hambly, B., and Fajer, P. G. (1999) Intradomain distances in the regulatory domain of the myosin head in prepower and postpower stroke states: fluorescence energy transfer. Biochemistry 38, 13,026–13,034.
Chantler, P. D. and Tao, T. (1986) Interhead fluorescence energy transfer between probes attached to translationally equivalent sites on the regulatory light chains of scallop myosin. J. Mol. Biol. 192, 87–99.
Kekic, M., Huang, W., Moens, P. D., Hambly, B. D., and dos Remedios, C. G. (1996) Distance measurements near the myosin head-rod junction using fluorescence spectroscopy. Biophys. J. 71, 40–47.
Suzuki, Y., Yasunaga, T., Ohkura, R., Wakabayashi, T., and Sutoh, K. (1998) Swing of the lever arm of a myosin motor at the isomerization and phosphate-release steps. Nature 396, 380–383.
Yasunaga, T., Suzuki, Y., Ohkura, R., Sutoh, K., and Wakabayashi, T. (2000) ATP-induced transconformation of myosin revealed by determining three-dimensional positions of fluorophores from fluorescence energy transfer measurements. J. Struct. Biol. 132, 6–18.
Root, D. D. (1997) In situ molecular asociation of dystrophin with actin revealed by sensitized emission immuno-resonance energy transfer. Proc. Natl. Acad. Sci. USA 94, 5685–5690.
Ha, T., Enderle, T., Ogletree, D. F., Chemla, D. S., Selvin, P. R., and Weiss, S. (1996) Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor. Proc. Natl. Acad. Sci. USA 93, 6264–6268.
Clegg, R. M., Murchie, A. I., Zechel, A., and Lilley, D. M. (1993) Observing the helical geometry of double-stranded DNA in solution by fluorescence resonance energy transfer. Proc. Natl. Acad. Sci. USA 90, 2994–2998.
Barden, J. A. and dos Remedios, C. G. (1984) The environment of the high-affinity cation binding site on actin and the separation between cation and ATP sites as revealed by proton NMR and fluorescence spectroscopy. J. Biochem. 96, 913–921.
Ando, T., Yamamoto, T., Kabayashi, N., and Munekata, E. (1992) Synthesis of a highly luminescent terbium chelate and its application to actin. Biochim. Biophys. Acta 1102, 186–196.
Burmeister Getz, E., Cooke, R., and Selvin, P. R. (1998) Luminescence resonance energy transfer measurements in myosin. Biophys. J. 74, 2451–2458.
Arata, T. (1996) A myosin head can interact with two chemically modified G-actin monomers at ATP-modulated multiple sites. Biochemistry 35, 16,061–16,068.
Wells, A. L., Lin, A. W., Chen, L. Q., Safer, D., Cain, S. M., Hasson, T., et al. (1999) Myosin VI is an actin-based motor that moves backwards. Nature 401, 505–508.
Brenner, B., Xu, S., Chalovich, J. M., and Yu, L. C. (1996) Radial equilibrium lengths of actomyosin cross-bridges in muscle. Biophys. J. 71, 2751–2758.
Tao, T., and Lamkin, M. (1981) Excitation energy transfer studies on the proximity between SH1 and the adenosinetriphosphatase site in myosin subfragment 1. Biochemistry 20, 5051–5055.
Cheung, H. C., Gonsoulin, F., and Garland, F. (1985) An investigation of the SH1-SH2 and SH1-ATPase distances in myosin subfragment-1 by resonance energy transfer using nanosecond fluorimetry. Biochim. Biophys. Acta. 832, 52–62.
Kensler, R. W. (2002) Mammalian cardiac muscle thick filaments: their periodicity and interactions with actin. Biophys. J. 82, 1497–1508.
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Root, D.D. The dance of actin and myosin. Cell Biochem Biophys 37, 111–139 (2002). https://doi.org/10.1385/CBB:37:2:111
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DOI: https://doi.org/10.1385/CBB:37:2:111