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Replication-related activities establish cohesion between sister chromatids

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Abstract

Replicated sister chromatids are held together from their synthesis in S phase to their separation in anaphase. The process of sister chromatid cohesion is essential for the proper segregation of chromosomes in eukaroytic cells. Recent studies in Saccharomyces cerevisiae have advanced our understanding of how sister chromatid cohesion is established, maintained, and dissolved during the cell cycle. Historical observations have suggested that establishment of cohesion is roughly coincident with replication fork passage. Emerging evidence now indicates that replication fork components, such as PCNA, a novel DNA polymerase, Trf4p/Pol σ (formerly Trf4p/Pol κ), and a modified clamp-loader complex, actively participate in the process of the cohesion establishment. Here, we review the molecular events in the chromosome cycle with respect to cohesion. Failure of sister chromatid cohesion results in the aneuploidy characteristic of many birth defects and tumors in humans.

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  1. Zhenghe Wang

    Present address: Molecular Genetics Lab, The Johns Hospkins Oncology Center, CRB 5th Floor, 1660 Oreleans St., 21231, Baltimore, MD

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  1. Department of Genetics and Genomics, Evans Bldg., Boston University School of Medicine, 715 Albany St., 02118, Boston, MA

    Zhenghe Wang & Michael F. Christman

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Wang, Z., Christman, M.F. Replication-related activities establish cohesion between sister chromatids. Cell Biochem Biophys 35, 289–301 (2001). https://doi.org/10.1385/CBB:35:3:289

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