Abstract
Photosystem II (PSII) particles were purified from Eu3+-treated spinach and studied by spectroscopy. The results showed that electron transport rate of PS II was accelerated by Eu3+ treating, that violet shift of the PSII Soret band or Q-band was 6 nm or 2 nm for the ultraviolet-visible (UV-Vis) spectrum, that the violet shift of the PSII fluorescence emission peak was 9 nm for fluorescence emission spectrum, that the PSII Signal II’s of low-temperature electron paramagnetic resonance (EPR) spectrum was intensified under light, and that the PSII CD spectrum was similar to that of control. It is suggested that Eu3+ might bind to the PSII reaction center complex and enhance the electron transport rate of PSII CD; however, Eu3+ treatment does not change the configuration of the PSII reaction center complex.
Similar content being viewed by others
References
J. Z. Ni, Rare Earth Bioinorganic Chemistry, Science Press, Beijing, pp. 13–37 (1995).
F. S. Hong, Z. G. Wei, Y. Tao, et al., Distribution of rare earth elements and structure characterization of chlorophyll-lanthanum in a natural plant fern Dicranopteris dichotoma, Acta Botan. Sin. 41(8), 851–854 (1999).
F. S. Hong, Z. G. Wei, and G. W. Zhao, Mechanism of lanthanum effect on the chlorophyll of spinach, Sci. China Ser. C 45(2), 166–175 (2002).
F. S. Hong, Z. G. Wei, and G. W. Zhao, Study of bound form on rare earths in a natural plant fern Dicranopteris dichotoma by spectroscopy, Chem. J. Chin. Univ. 22(11), 1790–1794 (2001).
F. S. Hong, Z. G. Wei, G. W. Zhao, et al., Extended X-ray absorption fine structure study of the bound form of lanthane in chlorophyll-a from Dicranopteris dichotoma, Biol. Trace Element Res. 82, 289–295 (2001).
F. S. Hong, L. Wong, X. X. Meng, et al., The effect of cerium on the chlorophyll formation of spinach, Biol. Trace Element Res. 89, 263–277 (2002).
F. S. Hong, X. E. Wang, C. Liu, et al., Effect of Ce3+ on spectral characteristic of D1/D2/Cytb559 complex from spinach, Sci. China Ser. B 46(1), 42–50 (2003).
X. S. Tang and K. Satoh, The oxygen-evolving photosystem II core complex, FEBS Lett. 179, 60–64 (1985).
O. Nanba and K. Satoh, Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559, Proc. Natl. Sci. USA 84, 109–112 (1987).
N. Yamaguchi, Y. Takahashi, and K. Satoh, Isolation and characterization of a photosystem II core complex depleted in the 43 kda-chlorophyll-binding subunit, Plant Cell Physiol. 29, 123–129 (1988).
H. Schiller, J. Dittmer, L. Luzzolino, et al., Structure and orientation of the oxygen-evolving manganese complexes of green algae and higher plants investigated by X-ray absorption linear dichroism spectroscopy on oriented photosystem II membrane particles, Biochemistry 37, 7340–7350 (1998).
L. Luzzolino, J. Dittmer, W. Dorner, et al., X-ray absorption spectroscopy on layered photosystem II membrane particles suggests mangnese-c entered oxidation of the oxygen-evolving complex for the S0–S1, S1–S2, and S2–S3 transitions of the water oxidation cycle, Biochemistry 37, 17,112–17,119 (1998).
L. C. Sun, M. K. Raymond, A. Magnuson, et al., Towards an artificial mode for ruthenium(II) tris-bipyridine via a tyrosine derivative, J. Inorg. Biochem. 78, 15–22 (2000).
T. K. Ono, Effects of lanthanide substitution at Ca2+-site on the properties of the oxygen evolving center of photosystem II, J. Inorg. Biochem. 82, 85–91 (2000).
C. A. Tracewel, A. Cua, D. H. Stewart, et al., Characterization of carotenoid and chlorophyll photooxidation in photosystem II, Biochemistry 40, 193–203 (2001).
D. I. Arnon, Copper enzymes in isolated chloroplasts: polyphenol oxidase in Beta vulgaris, Plant Physiol. 24, 1–15 (1949).
T. Kuwabara and N. Murata, Inactivation of photosynthetic oxygen evolution and concomitant release of three polypeptides in the photosystem II particles of spinach chloroplasts, Plant Cell Physiol. 23, 533–539 (1982).
A. Sampetro (ed.), Plant Physiological Teaching and Research Group in Bejing Agricultural University (translation), Plant Physiological Research Method, Science Press, Beijing, pp. 32–37 (1980).
A. Perczel, K. Park, and G. D. Fasman, Analysis of the circular dichroism spectrum of proteins using the convex constraint algorithm: a practical guide, Anal. Biochem. 203, 83–89 (1992).
J. Barber, D. J. Chapman, and A. Telfer, Characterization of a PSII reaction center isolated from the chloroplasts of Pisum sativum, FEBS Lett. 220, 67–73 (1987).
L. F. Du and X. S. Tang, Isolation and properties of oxygen-evolving photosystem II reaction center complex, Acta Phytophysiol. Sin. 18(1), 17–23 (1992).
M. Seibert, R. Picorel, and A. B. Rubin, Spectral, photophysical, and stability properties of isolated photosystem II reaction center, Plant Physiol. 87, 303–306 (1988).
L. X. Wang, Z. Xu, and X. Y. Wu, Effect of rare earth elements on photosynthesis and photofixiation of nitrogen in Anabena azotica, Chin. J. Rare Earths 3(3), 72–75 (1985).
A. Q. Zhang, L. X. Wang, X. Y. Wu, Effect of rare earth elements on photosynthesis, growth and hydrogen evolution of Spirulina platensis, Clin. J. Rare Earths 6(4), 55–88 (1988).
B. L. Shen and X. B. Dai, Effect of rare earths on photochemical reaction of chloroplasts of wheat, Rare Earths 15(2), 71–72 (1994).
F. L. Zeng, M. F. Zhang, and S. M. Zhou, The effect of lanthanide chloride on abscisic acid and electron-transport activity of some crop, Biol. Trace Element Res. 67, 277–285 (1999).
F. L. Zeng, Y. An, and L. Ren, Effect of lanthanum and calcium on photoelctron transport activity and the related protein complexes in chloroplast of cucumber leaves, Biol. Trace Element Res. 77, 83–91 (2000).
L. E. Andreasson and T. Vanngard, Electron trasport in photosystem I and II, Annu. Rev. Plant Physiol. Plant Mol. Biol. 39, 379–411 (1988).
V. K. Yachandra, R. D. Guiles, A. E. McDermott, et al., The state of manganese in the photosynthetic apparatus. 4. Structure of the manganese complex in photosystem II studied using EXAFS spectroscopy. The S1 state of the O2-evolving photosystem II complex from spinach, Biochim. Biophys. Acta 850, 324–332 (1986).
J. L. Cole, V. K. Yachandra, A. E. McDermott, et al., Structure of the manganese complex of photosystem II upon removal of the 33-kiloDalton extrinsic protein: an x-ray absorption spectroscopy study, Biochemistry 26, 5967–5973 (1987).
V. K. Yachandra, R. D. Guiles, A. E. McDermott, et al., Comparison of the structure of the manganese complex in the S1 and S2 state of the photosynthetic O2-evolving complex: an x-ray abosorption spectroscopy study, Biochemistry 26, 5974–5981 (1987).
E. Nabedryk, P. Biaudet, and S. Darr, Conformation and orientation of chl-proteins in photosystem I by circular dichroism and polarized infrared spectroscopies, Biochim. Biophys. Acta 767, 640–647 (1984).
V. G. Hinz, Isolation of the photosystem II reaction center complex from barley, characterization by circular dichroism spectroscopy and amino acid sequencing, Carlsberg Res. Commun. 50, 285–298 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fashui, H. Effect of Eu3+ on characterization of photosystem II particles from spinach by spectroscopy. Biol Trace Elem Res 97, 279–288 (2004). https://doi.org/10.1385/BTER:97:3:279
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/BTER:97:3:279