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Effect of Eu3+ on characterization of photosystem II particles from spinach by spectroscopy

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Abstract

Photosystem II (PSII) particles were purified from Eu3+-treated spinach and studied by spectroscopy. The results showed that electron transport rate of PS II was accelerated by Eu3+ treating, that violet shift of the PSII Soret band or Q-band was 6 nm or 2 nm for the ultraviolet-visible (UV-Vis) spectrum, that the violet shift of the PSII fluorescence emission peak was 9 nm for fluorescence emission spectrum, that the PSII Signal II’s of low-temperature electron paramagnetic resonance (EPR) spectrum was intensified under light, and that the PSII CD spectrum was similar to that of control. It is suggested that Eu3+ might bind to the PSII reaction center complex and enhance the electron transport rate of PSII CD; however, Eu3+ treatment does not change the configuration of the PSII reaction center complex.

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Fashui, H. Effect of Eu3+ on characterization of photosystem II particles from spinach by spectroscopy. Biol Trace Elem Res 97, 279–288 (2004). https://doi.org/10.1385/BTER:97:3:279

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  • DOI: https://doi.org/10.1385/BTER:97:3:279

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